apramycin 中文意思是什麼

apramycin 解釋
阿布拉黴素（安普黴素,阿泊拉黴素）
  1. Arginine feeding experiment showed that nitrogen metabolism in the s. tenebraius was obviously affected by arginine through two possible ways : ( l ) pronase activity in vitro could be influnced by arginine, as a result, the catabolism of nitrogen - containing macro - molecule was promoted and the nitrogen element in the broth was increased. ( 2 ) arginine could be transformed into glutamic acid, so that the biosynthesis of apramycin was promoted

    因而我們認為gln可能是安普黴素生物合成氮元素的供體。 arg添加實驗結果表明， arg可能通過兩種途徑影響黑暗鏈黴菌體內的氮代謝： （ 1 ） arg可能影響胞外蛋白酶的活性，進而促進含氮大分子物質的分解代謝，補充發酵過程中的氮素來源。
  2. In this study, the avermectin - producing strain streptomyces avermitilis was studied and the avermectin biosynthesis gene cluster in the genomic dna of streptomyces avermitilis s - 2 was altered by the method of gene engineering. insertion inactivation of aved gene in the cluster by introducing apramycin resistance gene into aved gene resulted in the disappearance of " a " components of avermectins. when avec gene was inactivated by the same way, four " 1 " components were lost and only " 2 " components, the potential precursor of ivermectin, were accumulated

    將該基因簇中的aved基因通過插入外源的安普黴素抗性基因片段使其失活，導致發酵產物中4個a組分（不需要的組分）的消失；將基因簇中的avec基因通過同樣手段，使其失活，導致發酵產物中4個「 1 」組分的消失，而主要積累「 2 」組分（進一步改造可成為伊維菌素的前體b _ 2組分） 。
  3. The tertiary fermentation techniques and the fbc ( fed - batch culture ) and other fermentation techniques were also optimized. a scale - up fermentation technique was set up and the apramycin productivity reached 4478u / ml in a 4m3 fermentator

    對三級發酵工藝、補料工藝等發酵工藝條件進行了優化，進一步確定了中試發酵工藝條件， 4m ~ 3發酵罐上平均發酵單位達4478u ml 。
  4. Based on a 3. 1kb pst i fragment of genomic dna of a wild s. avermitilis, a 1. 5 kb apramycin resistance fragment was inserted into sph i site of avec gene in the 3. 1 kb fragment, then a recombinant plasmid pc05 was obtained by introducing above inactivated avec fragment into mcs region of phjl401. competent cells of et12567 were transformed by recombinant plasmid pid03 and pc05 respectively

    以含有avec基因的3 . 1kb基因組dnapsti片段為基礎，將1 . 5kb的安普黴素抗性基因片段插入到avec基因中的sphi酶切位點，再將此插入失活的avec基因片段連接到具有接合轉移功能（含有orit基因）的鏈黴菌-大腸桿菌穿梭質粒phjl401的多克隆位點區，由此得到重組質粒pc05 。
  5. Streptomyces tenebrarius a04, the producer ( with a titer of 2874u / ml ) of single component of nebramycin ? apramycin was studied in this paper. after treatment of spore suspension and mycelia shivered by supersonic with mutagen, combined with the application of screening models, some stable high yield apramycin - producing strains ( with a fermentation titer of 4800 - 5200u / ml by shaking flask ) such as a2 - 23, a2 - 30, asm6 and al - 16 were obtained

    本文以尼拉黴素單一組分?安普黴素產生菌s . tenebrariusa04 （發酵單位為2847u ml ）為出發菌株，通過對單孢子懸液和超聲波破碎菌體的誘變處理並復合篩選模型，獲得了遺傳特性穩定的單組分高產菌株： a2 - 23 、 a2 - 30 、 asm6 、 a1 - 16 ，搖瓶發酵單位達4800 - 5200u ml 。
  6. It was also proved that the biosynthestic genes of apramycin was linked to the apramycin resistant gene in s. tenebrarius

    Pcr實驗證明基因重組菌株中接合轉移質粒同源整合到黑暗鏈黴菌h6的染色體上。
  7. The gene recombinant strain no. 42 ca n ' t generate ampramycin, which indicated that the cloned gene is involved in apramycin biosynthesis in s. tenebrarius

    通過接合轉移的方法將質粒pzxb014導入黑暗鏈黴菌h6中，篩選基因發生重組的菌株。
  8. A 1. 5 kb apramycin resistance fragment was inserted into nru i site of aved gene and the inactivated aved gene fragment was then introduced into mcs region of phjl401 - an e. coli / streptomyces shuttle vector with conjugation function ( containing orit gene ). as a result of above procedures, a recombinant plasmid pid03 was obtained

    將1 . 5kb的安普黴素抗性基因片段插入到aved基因中的nrui酶切位點，再將此滅活的aved基因片段插入到具有接合轉移功能（含有orit基因）的鏈黴菌?大腸桿菌穿梭質粒phjl401的多克隆位點區，由此得到重組質粒pid03 。
  9. Amino acid feeding experiments were also performed in this study in order to investigate the pathway, by which nitrogen incoperated into apramycin molecule, and the regulatory mechanism of nitrogen metabolism

    通過氨基酸添加實驗研究了安普黴素生物合成途徑中的氮的來源及調節機制。
  10. To study the biosynthetics genes of apramycin in streptomyces tenebrarius, the apramycin resistant gene was isolated by gunshot cloning firstly

    為了研究黑暗鏈黴菌安普黴素生物合成基因，首先通過鳥槍克隆的方法克隆其安普黴素抗性基因。
  11. The feeding of glutamic acid, glutamine and a - keto - glutaric acid respectively showed that glutamic acid and glutamine had no obvious effects on cell growth, but stimulated apramycin production greatly. with the same concentration of amino acids supplemented, glutamine showed a stronger stimulation effect than glutamic acid, while a - keto - glutaric acid showed a repression effect on apramycin production. it could be deduced from above results that glutamine possibly is the donor of nitrogen element for the biosynthesis of apramycin

    Glu 、 gln及?酮戊二酸添加實驗結果表明glu 、 gln對菌體的生長無明顯的影響，但能強烈促進安普黴素的生物合成，且相同濃度條件下gln的促進作用明顯高於glu ，而?酮戊二酸則對安普黴素的生物合成有抑制作用。
  12. The ligation mixture was used to transform s. lividans tk24 protoplasts. six colonies capable of growing in the presence of apramycin were isolated

    連接液轉化標準宿主菌s . lividanstk24原生質體，最終得到6個可以在安普黴素平板上生長的陽性克隆。
  13. This result proved that uv treatment combined with tolerating apramycin or with tolerating streptomycin could be effective way to obtain high yield apramycin - producing strains

    證明了uv處理復合耐受自身產物、 uv處理復合耐受鏈黴素能獲得較好的篩選結果。
  14. The 2. 0 - kb bamhl - sphl fragment, upstream fragment of the apramycin resistant gene, was sequenced. analysis of the nt sequence revealed that it contained an orf from nt positions 877 to 1995

    抗性基因上游2 . 0kbbamhi - sphi片段測序表明在該片段中有一個完整的orf ，位於877 1995bp之間，編碼373個氨基酸。
  15. Plasmid psyxl, containing a 12kb insert of s. tenebrarius dna, was isolated from one of six colonies. apramycin resistant gene was located in 1. 5 - kb sphl - kpnl fragment of plasmid psyxl though further studies

    陽性克隆中重組質粒psyx1含有12kb黑暗鏈黴菌的dna ，對12kb片段進一步分析將安普黴素抗性基因定位在1 . 5kbsphi - kpni片段上。
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