assay value 中文意思是什麼

The application value of optical platelet assay with sysmex xe - 2100 blood analyzer

2100血液分析儀光學血小板檢測的應用價值

The practical value of optical assay of platelet count by sysmex xe - 2100 hematoloical analyzer in abnormal platelet particle size distribution histogram

2100血液分析儀光學法計數血小板在血小板直方圖異常時的應用價值

Establishment of paraoxonase activity assay method and clinical value

人血清對氧磷酶活性測定方法的建立及臨床初步應用

The recombinants were constructed by transforming ppic9 a - xynb into p. pastoris gs115. the assay results revealed that the xylanase gene xynb was overexpressed and secreted effectually in p. pastoris. in 3l fermentor the expression level of xylanase xynba exceeded 1200iu / ml and the expressed xylanase had normal bioactivity. the molecule weight of xynba was determined as about 31kd which is higher than 23kd of original enzyme xynb from streptomyces olivaceoviridis a1. xynbb was gotten by deglycasylation of xynba, whose molecule weight returned to 23kd. we comparised the enzymatic properties of xynba expressed in p. pastoris, xynbb deglycasylated from xynba and xynb produced from streptomyces olivaceoviridis al : there was little difference among the three enzymes on optimal ph, the optimal ph of xynb and xynba were both 5. 2, the optimal ph of xynbb was 5. 0 ; the optimal temperature of xynb and xynba were both 60 c, while the optimal temperature of xynbb was 50 ? ; because of glycosylation the thermal stability of xynba was better than xynb and xynbb ; the specific activity of xynba and xynbb were 883. 88iu / mg and 832. 5hu / mg respectively, which were both lower than 2814. 45iu / mg of xynb ; the km values of xynb and xynba were similar to each other which were 21. 56 ( g / kg ) and 20. 87 ( g / kg ), while the km value of xynbb was 27. 10 ( g / kg ) ; the fmax of xynba and xynbb were 4568umol / mg. min and 5329umol / mg. min respectively which were lower than 27623 umol / mg. min of xynb ; additionally all of the three enzymes did not display cellulase activity. they all had well resistance to pepsion and trypsin, and were not sensitive to metal iron, surface active agent and chelating agent. the analysis of different xylans enzymatic hydrolysate revealed : by xynba, that the main constitutions of enzymatic hydrolysate of birch wood xylans were xylotriose and xyloquaiose, which account for 68. 43 % and 16. 50 % respectively, additionally there was 11. 79 % of xylobiose ; the main constitutions of enzymatic hydrolysate of corncobs xylans were xylobiose and xylotriose, which account for 81. 78 % and 11. 55 %. the result indicated that this xylanase was a kind of 1, 4 - b - d - xylanohydrolase and was fit to used in industrial procession of xylooligosacc harides

進一步對xynba進行了脫糖基化處理得到xynbb ，其分子量恢復到23kd ，證明xynba是糖基化蛋白。通過對畢赤酵母重組表達的木聚糖酶xynba 、脫糖基化的木聚糖酶xynbb以及橄欖綠鏈黴菌a1所產原酶xynb之間酶學性質的比較發現：三種酶的最適ph差異不大， xynb和xynba均為5 . 2 ， xynbb為5 . 0 ； xynb和xynba的最適溫度均為60 ， xynbb降為50 ：在耐熱性上， xynba由於糖基化作用熱穩定性明顯高於未糖基化的xynb和xynbb ； xynba和xynbb的比活性分別為883 . 88iu mg和832 . 51iu mg ，明顯低於原酶的比活2814 . 45iu mg ； xynb和xynba的km值相當，分別為21 . 56 （ g kg ）和20 . 87 （ g kg ） ，而xynbb的km值較大為27 . 10 （ g kg ） ； xynba和xynbb的vmax相差不大，分別為4568 mol mg ? min和5329 mol mg ? min ，明顯低於xynb的27623 mol mg ? min此外三種酶均無纖維素酶活性，對胃蛋白酶和胰蛋白酶有很好的抗性，且對作用環境中的各種離子、表面活性劑、螯合劑不敏感。通過對不同木聚糖的酶解產物的糖份分析發現：以樺木木聚糖為底物時，酶解產物主要為木三糖和木四糖，含量分別為68 . 43和16 . 50 ，另外還含有11 . 79的木二糖；以玉米芯木聚糖為底物時，酶解產物主要為木二糖和木三糖，含量分別為81 . 78和11 . 55 。

Anti - melatonin monoclonal antibodies of higher titer, affinity and good sensitivity were obtained by coupling mt to bovine serum albumin with formaldehyde and by immunizing mice with multifocal intra - dermal injections. we obtained 6 strains of hybridoma, all of them secreting specific antibodies to mt, we apply antibodies to determinate free mt inhuman serun with group - selective immunoassay technique. an inhibition curve for mt was obtained in the range of 50pg to30ng, and 1. 4ng of mt inhibited the value of the assay by half. we evaluate the specificity of antibodies by determination of cross - reactivity of several analogues, the moabs recognized mt but

通過將mt用甲醛作連結劑連結到牛血清白蛋白上sa採用皮下多點注射兔疫小鼠得到了高效價，高親和力，較好特異性的抗mt單克隆抗體，最後獲得了5株單克隆細胞株，都能分泌針對mt的特異性抗體，建立了選擇性基團免疫分析法，用制備的抗體測定了人血清中mt的含量，作了mt的抑制標準曲線，其抑制范圍從50pg ? 30ng ，半抑制量為1

Integral scientific method ensure larg - spaned enhance in teaching and learning of chinese language and literature ( clltl ). this argument are based on full course improved trial of cltl in no. l high middle - school affiliated to central china normal university. according to combination of practice and theory, the assay details such conceptions as following : essential meaning, theoretical base, applied value and operational route during full course

本文以華中師大一附中語文教學運行體系全程優化實驗為基礎，結合作者的教學實踐和理論思考，具體闡述了語文教學運行體系全程優化的本質含義、理論基礎、應用價值和實施途徑，以期為語文教學的最優化提供可借鑒的現實模式。

Study on the immunodiagnostic value of the enzyme - linked immunosorbent assay in giardiasis

用酶聯免疫吸附試驗診斷藍氏賈第鞭毛蟲病免疫價值的研究

2 mtt assay was used to examine od value of terasaki wells. od value was measured once a day for 8 days and mapped growing curve of cells to determine the activation of proliferation in primary cells

2採用mtt比色法測定細胞的光密度值（ 0d值） ，每24h測定1次，從細胞接種到神經球形成連續測定sd ，繪制細胞增殖活性曲線。

A high - density molecular linkage map will be of value for studies designed to assay genetic variation, identify markers associated with dissect quantitative trait loci, and perform marker - assisted selection to improve agronomically important traits within plants

摘要高密度分子連鎖遺傳圖譜對分析植物遺傳變異、標記目標性狀、數量性狀定位和分子輔助選擇改良性狀均具重要價值。

The solution was adjusted to ph value of 6. 5, followed by extracted with ethyl acetate, evaporated to dryness and dissolved the residue with mobile phase. the solution was subsequently subjected to separate hplc assay for each drug

用稀醋酸溶液（ 0 . 01mol l ）調節ph至6 . 5 ，以乙酸乙酯提取，提取液經減壓濃縮至近干，以流動相溶解定容，然後用高效液相色譜測定。

These results demonstrate that the dot - immunogold assay shows its practical value to monitor the brucella infections among the epidemic area and individuals with different occupations in the un - epdemic area, and this method can also be applied for the clinical investigation and epidemiological survey of brucellosis

結論滴金免疫測定法對布病疫區人群和非疫區重點職業人員開展布病監測具有實用價值，也可用於布病的臨床檢測和流行病學篩查。

The first part, flow cytometry assay research for molecules in the surface of platelets section l flow cytometry methodology research for detecting cd62p reserve of preserved platelets stimulated with thrombin objective 1 ) to establish and optimize a flow cytometry method which is used to test cd62p reserve incubated with thrombin ; 2 ) daily determinating the cd62p reserve of platelets stored at 22 ? to assess its value in evaluation of preserved platelet quality

研究主要分為四部分：第一部分，血小板膜表面分子流式細胞術分析新方法和指標的建立第一節，流式細胞術測定保存血小板再表達cd62p方法的建立目的1建立並優化測定血小板再表達膜表面激活標志物cd62p能力的流式細胞術（ fcm ）方法。

It goes on with discussing the possible challenges the contemporary iit for us, like causing our country to stay at the low part in the industrial value chain, the technology bogging down, resources losing, ecological environment worsening, and so on. bases on the above analysis, this assay at last has proposed some counter - measures to the contemporary iit. namely : in the foundation of grasping the characteristics and new tendencies of the iit and integrated with our country ’ s comprehensive superiorities, we can response from implementing

在此基礎上，本文最後提出了我國應對當代國際產業轉移的基本思路及對策，即在把握當代國際產業轉移特點和趨勢的基礎上，結合我國所具有的綜合優勢，從政府政策把關，合理引導國際產業轉入，到培育我國的自主技術創新能力和體系建設，再到促進服務業的發展以及適時適度開展產業轉移加以應對。

The experimental group were muscularyly injected with il - 2 at intervals. the results were as follows : ( 1 ) the results of cellular imnune assay ( mtt ) the sixth week after the vaccination, peripheral blood lymphocytes isolated were tested by iftt. the result showed that the nonspecific od value and the specific ( x ) value of guinea pigs of il - 2 group was significantly higher than those of bcg and sham injection group

結果發現： （ 1 ）免疫第二周后， bcg + il - 2組特異性和非特異性淋巴細胞轉化實驗所測ltt - od值與bcg組和空白組無明顯差異（ p 0 . 05 ） ，第四周時檢測數值開始出現升高，第六周時ltt檢測結果顯示il - 2 + bcg組的特異性和非特異性的ltt - od值極顯著高於bcg組和空白對照組（ p 0 . 01 ） 。

First, simply introduce the methods of calculation in the reliability. and introduce the rationale and the course of the calculation of the stress and the stability of arm ' s base in fixed value as a rule. also assay the stress and the stability of arm ' s base in fixed value with a great finite element method

首先簡要介紹了可靠度的基本計算方法；以及用定值方法分析拱壩應力及壩肩穩定的基本原理和一般過程，並運用某大型有限元程序對拱壩的應力和抗滑穩定進行定值分析。