bacterial digestion 中文意思是什麼
bacterial digestion
解釋
細菌消化-
Bacterial digestion may continue.
細菌可能繼續分解。 -
In this study, we designed a pair of primers based on the sequence of the upstream and downstream of chicken il - 2 gene. about 600 bp chicken il - 2 cdna fragment was cloned from cona - stimulated chicken splenocytes by reverse transcription - polymerase chain reaction ( rt - pcr ) and was subcloned into puc18 vector. recombinant clone was demonstrated by restriction enzyme digestion and dna sequencing. next, we construct recombinant plasmid pproex ? t - il - 2. the cdna of chicken il - 2 gene was subcloned into bamh i / hind iii sites of vector. the recombinant plasmid pproex ? t - il - 2 was transformed into e. coli dh5a and the bacteria was induced with iptg. it was demonstrated by sds - page and western blot that a 18kda protein which was equal to chicken il - 2 protein in molecular weight was expressed in e. coli dh5a. the expression level was up to 30 % of the total bacterial proteins. the purified protein was used to prepare the antibody against chicken il - 2 protein
經酶切鑒定及dna序列測定,該基因為雞il - 2基因,其序列與sundick等報道的完全一致。在此基礎上,我們把雞il - 2基因亞克隆到大腸桿菌原核表達載體pproex ~ ( tm ) ht中,構建重組表達質粒並進行確證性序列測定,重組質粒測序結果表明將編碼雞il - 2成熟蛋白的基因正確地插入到原核表達載體pproex ~ ( tm ) ht的目的位點。重組質粒轉化受體菌dh5後用iptg於37進行誘導培養, sds - page和westernblot分析顯示,表達的雞il - 2融合蛋白分子量約為18kda ,表達的融合蛋白經薄層掃描發現目的蛋白表達量約占菌體蛋白的30 。
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