baculovirus 中文意思是什麼

baculovirus 解釋
棒狀病毒科
  1. Usin g a silkworm baculovirus expression vector system ( bevs ), a large amount of appa phytase was obtained ( up to 7710 per ml hemolymph )

    感染五齡家蠶后appa植酸酶的表達量達到平均每毫升血淋巴7710u 。
  2. Both the two phytase had a optimal temperature and ph at 60 ? and ph 4. 5 except the baculovirus derived phytase seemed to be more thermostable

    兩個表達系統中表達的重組appa植酸酶的最適溫度與最適ph一致，分別為60與ph4 . 5 。
  3. Production of insulin - expressing adeno - associated virus using baculovirus system

    利用昆蟲病毒系統生產表達胰島素的腺相關病毒
  4. Construction of baculovirus transfer vectors for expression of core antigens of newcastle disease virus

    核心抗原重組桿狀病毒轉移載體的構建
  5. Yu - chan chao at the institute of molecular biology, academia sinica, constructed a recombinant baculovirus

    在貼附培養的情況下，此病毒感染宿主昆蟲細胞不會造成昆蟲細胞溶裂。
  6. The recombinant baculovirus harboring the appa gene was obtained after co - transfection and several screening rounds

    用乳糖誘導使appa基因在大腸桿菌菌株bl21中得到了高效表達。
  7. Baculovirus - expressed system and research of infectious bursal disease virus gene expressed in a baculovirus recombinant

    桿狀病毒表達系統及其表達傳染性法氏囊病病毒基因研究進展
  8. In the wild type hasnpv bvs, no detectable multimer was found. however, in the pseudotyping baculovirus bvs, three oligomer bands were found besides

    在hasnpvbv中沒有發現ha133的多聚體，然而該重組病毒bv中卻有三種ha133的多聚體存在。
  9. They are also the key components of insect - baculovirus expression system ( bevs ), which has been one of the four main expression systems in gene engineering

    同時，桿狀病毒昆蟲細胞表達系統是一類重要的真核表達系統，現已成為目前基因工程四大表達系統之一。
  10. Second, some reports utilized the baculovirus as a gene delivery vehicle into mammalian cells and did not have any bad effect on the growth of mammalian cell

    因此，本論文中討論一些影響蛋白質表現量與延長表現的參數如:培養基的更換、重復感染、病毒感染方式。
  11. The recombinant transfer vector pbacpak - hbmp was constructed by insertion of the hbmp coding sequences into the multiple cloning site of transfer vector pbacpak. 8. bmn cell line was co - transfected with pbacpak - hbmp plasmid and linearized baculovirus bacpak6 dna by dosper agent

    將克隆到的hbmp基因通過適當的酶切插入到轉移載體質粒pbac - pak8的多克隆位點中，獲得重組轉移載體質粒pbacpak - hbmp 。
  12. The reasults are summed up as following : 1 the study on chromosomes and mitoses of bmn cells the cell line, bmn, is a silkworm cell line widely used in silkworm molecular genetics, cell engineering, gene engineering and baculovirus expression system but whose genetics and cytobiology studies are nearly untouched. the chromosomes and mitoses of the bmn cells are researched by the air - drying method and culturing cells on cover glasses

    同時，還通過原代培養實驗對新的家蠶胚胎細胞系的建立進行了探索和嘗試，並對家蠶胚胎原代培養過程中出現的細胞和組織類型進行了觀察、探討與研究。 1bmn細胞有絲分裂及染色體研究bmn細胞是家蠶分子遺傳學，細胞工程、基因工程和桿狀病毒表達系統中廣泛應用的家蠶細胞，但其遺傳學和細胞生物學背景知之甚少。
  13. Baculovirus / insect cell system has been widely used for recombinant protein production, but traditional system eventually resulted in cell lysis, so that the expressed recombinant protein was lost into medium

    摘要:桿狀病毒/昆蟲細胞系統已經被廣泛的應用在重組蛋白質的生產上，但傳統的桿狀病毒感染后會造成細胞溶裂，而使得表現出的重組蛋白質流失到培養基中。
  14. Compared hasnpv helicase with the helicases of autographa colifornica mnpv ( acmnpv ), ( bombyx bori npv ( bmnpv ), lymantria dispar mnpv ( ldmnpv ) spodoptera exigue mnpv ( semnpv ), orgyia pseudotsugata mnpv ( opmnpv ), and xestia c - nigrum granuloviurs ( xcgv ), only 5 motifs ( i, la, ii, iii, iv ) were found conserved in baculovirus

    其在終止密碼子下游第12位有? polya終止信號aataaa 。 hasnpv解螺旋酶與其它5種桿狀病毒解螺旋酶相比，有5個基元序列（ 、 a 、 、 、 ）保守，另外兩個（和）完全不保守。
  15. Meq protein, highly expressed in the insect cell line sf9 by the baculovirus vector was immunized into balb / c mice and the immunized spleen cells were collected and fused with the tumor cell line sp2 / 0 via peg - 1000 in vitro. the hybridoma cells were cloned and screened for the ability of anti - meq mcab secretion by fa with the mdv ga infected chicken embryo fibroblast ( cef )

    利用通過桿狀病毒載體在昆蟲細胞系sfg上高度表達的meq蛋白產物免疫balb / c小鼠，然後收獲其免疫脾細胞並與腫瘤細胞系spz / 0通過peg于體外融合;獲得的雜交瘤細胞被克隆並通過與mdv感染的雞胚成纖維細胞（ cef ）做免疫熒光試驗（ fa ） ，進行其分泌抗meq單克隆抗體（ mcab ）能力的篩選。
  16. Results we construct recombinant angiostatin baculovirus with a high virus titer ( 2 + 108 pfu / ml ) successfully. recombinant angiostatin was effectively expressed in insect cells ( sf9 ) as 53 kd fusing protein and its expression level was about 90 % of insect cellular total soluble proteins. the recombinant angiostatin protein could inhibit endothelial cell proliferation in vitro with ic50 value of 2

    實驗結果我們成功地構建了滴度高達2x10 『 pm llil的angiostatin重組? 2 ?桿狀病毒，並在昆蟲細胞sffi中高效表達了分子量為53kd的an giostatin重組蛋白，重組angiostatin蛋白不僅在體外顯著抑制內皮細胞的生長， k 。
  17. The homology of recombinant virus bmpak - hbmp was obtained and identified by plaque assay and baculovirus contains the hbmp gene was confirmed through pcr and dna dot blotting. the expressed rhbsag was determined by elisa after infecting bm - n cells and pupae with recombinant virus bmpak - hbmp and bmpak - hbm ( containing nonfusion hbv surface antigen medium sized )

    用重組病毒bmpak - hbmp和bmpak - hbm [帶有非融合乙肝表面抗原（ pres2 + s ）基因，為本實驗構建]感染家蠶細胞及蛹，對兩種病毒的表達產物用elisa進行了跟蹤檢測，結果表明，感染bmpak - hbmp的家蠶細胞及蛹中rhbsag的表達量分別為3
  18. The protein product of meq gene was highly expressed in the nuclei of recombinant baculovirus infected sf9 cells when using an anti - meq monoclonal antibody ( mcab ) 23b46 to run the immunofluorescence assay ( fa ) ; the expression quantity and if staining patterns differed with different times post - infection ( pi ). the results of western blotting and immunoprecipitation test showed there were two specific bands around 60 kd. the results of the study demonstrated that the baculovirus / insect cell system is effective to be used to express nuclear protein of virus

    結果發現:本表達系統產生的meq蛋白可被重組痘病毒表達的meq制備的單抗23b46所識別;在感染細胞中， meq蛋白僅局限於細胞核內，而且隨著感染后（ pi ）時間的增加，具有從核質向核仁和核膜轉移的趨向; w已stemblot和免疫沉澱試驗均證實重組桿狀病毒感染細胞裂解物中出現有兩條大小約為60kd的特異帶。
  19. The pbacph - egf plasmid dna was used to co - transfect bmn cell with the modified bombyx mori baculovirus bm - bacpak dna, which was first linearized by aocl. after two rounds of plaque isolation, the recombinant virus ( named bmbacph - egf ) was further verified with pcr and dot hybridization. the recombinant bmbacph - egf virus was used to infect bmn culture cells at a moi of 10

    重組轉移載體dna與經bsu36i酶切線性化的修飾型病毒bm - bacpakdna共轉染家蠶bmn細胞，兩輪空斑篩選和純化，獲得重組病毒rbmbacph - egf ，經pcr擴增、 dna點雜交等方法鑒定證實重組病毒中已正確插入ph - egf融合基因。
  20. The angiostatin baculovirus transfer vector was co - transfected with viral dna into sf9 cells according to the manufacturers protocol. to purify the recombinant virus, we used the plaque assay to screen the pure recombinant plaque and amplify it to generate p - 1 stock

    構建重組病毒：用已經構建好的angiostatin桿狀病毒轉移載體pbluebachiszb和病毒dna共同轉染sfg細胞，通過蝕斑實驗篩選出純的重組斑點並擴增產生p二病毒貯存液。
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