biochemical extraction 中文意思是什麼
biochemical extraction
解釋
生化提取（法）- biochemical : adj. 生物化學的。n. 生物化學物質。adv. -ly
- extraction : n. 1. 抽出，拔出。2. 【化學】提取（法）；萃取（法）；回收物，提出物；精煉。3. 精選，摘要。4. 血統，家世，出身。5. 【數學】開方，求根。
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Water quality - physical, chemical and biochemical methods - determination of selected phenoxyalkanoic herbicides, including bentazones and hydroxybenzonitriles by gas chromatography and mass spectrometry after solid phase extraction and derivitization
水質.物理化學和生物化學法.固相萃取和衍生化作用后氣相色譜質譜法測定包括苯達松和羥基苯甲晴在內的選擇性苯氧烷類鋤草劑 -
Hisityl - trna synthetase catalyzes the aminoacylation of trnahis in the initial step of protein biosynthesis. the involumen of histidyl - trna synthetase in autoimmune diseases is another feature of the enzyme. in studies, it is reported that purified jo - 1 antigen can increase the detection rate of anti - jo - 1 antibody. but it is difficult to obtain a single component by biochemical extraction. genetic engineering can help us to desolve this problem. after looking up mrna sequence encoding histidyl - trna synthetase in genebank, we used rt - pcr technology to gain its full length dna sequence. the vestor ptybllwas used in the construction of expressing vestor. we transformed the jo - 1 gene into er2566 and used this system to express fusion jo - 1 antigen
本實驗從人胎盤中提取總rna ，利用rt - pcr技術獲得了編碼jo - 1的基因整長序列，選用impact - cn系統中的ptyb11載體，構建了jo - 1基因的克隆與表達載體，並轉化大腸桿菌er2566 ，經過抗性篩選、分子量大小比較、雙酶切鑒定、和pcr鑒定等多種方法驗證，篩選出了5個陽性克隆。
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