blocking antibody 中文意思是什麼
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We hope then to use the antibody as a vaccine against this blocking action.
我們希望以後能將這種抗體作為對抗這種阻滯作用的疫苗。 -
Get the inclusion bodies 2 ) western blot analysis of fusion protein expression ( 1 ) electrophoresis ( 2 ) transfer proteins from gel to membrane ( 3 ) blocking ( 4 ) incubation with primary antibody ( 5 ) enzyme conjugate incubation ( 6 ) substrate incubation. 3 ) gst detection module with cdnb enzymatic assay 3 purification of gst fusion proteins 1 the denaturalization of inclusion bodies. 2 purification using glutathione sepharose 4b column wash matrix with 1 pbs, prepare a 50 % slurry for batch purification method, pack column with matrix slurry
三、 gst一hbrp重組蛋白的純化1 .超聲破碎細胞，離心，上清和沉澱進行sds一page電泳分析2 .樣品處理提取包涵體，變性后，加人用pbs平衡過的以utal腸onesepb抓脫4b ，室溫下孵育3 .以utadtionesepharose4b柱純化以utad雲onesepharose4b柱的準備根據毛山lel ，決定純化所需的gll衛ta1如oneseph娜se4b的柱床體積，用預冷的1xpbs清洗cldtathionese戶， se4b ，得到50 %的基質 -
The results of the first part showed that both cd55 and cd59 neutralizing antibody can enhance the lysis or neutralization activity of complement. however, since rca are distributed widely, techniques that can mediate target rca blocking on virions are necessary
最後，病毒中和功能實驗結果顯示，我們制備的雙特異性抗體具有良好的誘導補體活化，中和eev病毒感染的作用，與親本抗體相比有增強的eev病毒中和活性。 -
Finally, in order to investigate whether these two virus are resistant to complement attack, and whether the resistance were mediated by incorporating cellular complement regulators of cd55, cd59 into their out membrane, complement lysis assay and plaque reduction assay were carried out respectively to find the lysis or neutralization activity of complement to hiv and eev under the condition of with or without the cd55 and cd59 blocking antibody
將這兩種病毒分別暴露於人血清補體后，發現在有cd55 、 cd59中和性抗體存在的情況下，補體溶解hiv病毒的效率明顯增加； eev病毒的感染活性在空斑實驗中也明顯降低，結果與這兩種蛋白在病毒表面（ eev ）存在的事實相符，提示病毒表面的cd55 -
Fortunately, recently widely used bispecific antibody technique provided us a good model for target rca blocking for it ' s dual antigen binding activity and for the availablity of high
為了進一步探討用基因工程的方法制備抗病毒雙特異性抗體的可能性，我們又在現有的條件下構建、表達了 -
Objective the aim of this article was to induce long term survival allografts by blocking cd40 - cd40l pathway with homemade anti - cd40l monoclonal antibody mr1, then purify cd4 + cd25 + regulatory t cells from recipients with long term survival allografts, and adoptively cotransfer such cells together with tcr transgenic allogenic antigen specific cd8 + t cells into immunodeffient mouse. finally study whether cd4 + cd25 + t cells can suppress allogenic antigen specific cd8 + t cell mediated rejection, thus clarified whether cd4 + cd25 + t cell mediated regulation is one of mechanisms of prolongation of allograft survival by cd40 - - cd40l pathway blockade
從移植物長期存活的受體內分選出cd4 + cd25 + t細胞，與tcr轉基因cds + t細胞同時過繼輸注到免疫缺陷小鼠體內，觀察co4 + co25 + t細胞對同種異體反應性cos + t細胞介導排斥反應的作用，探討在阻斷cd40se一~ cd40l共刺激途徑延長移植物存活中， cd4 + cd25 +調節性丁細胞是否是抑制排斥反應的機制之一。
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