blot hybridization 中文意思是什麼

blot hybridization 解釋
吸印雜交
  • blot : n 1 墨污，墨漬，污點，污斑。2 瑕疵；恥辱，污名。3 〈古語〉塗去，抹去。vt （ tt ）1 用（墨水等）弄...
  • hybridization : 混成作用
  1. The more accurate localization of 3galt - l mrna expression in mouse brain as studied by in situ hybridization was in good agreement with the general expression pattern seen with northern blot hybridization. in newborn mice, the dense granular layer and the purkinje cell showed a strong signal

    我們發現在小腦中1型鏈結構的變化與邵galt一1的表達變化基本一致:在新生鼠小腦中， 1型鏈結構在蒲肯野細胞和顆粒層細胞中都有表達，而在成年鼠小腦中， 1型鏈結構僅在蒲肯野細胞中表達。
  2. Nogo - 66 receptor, ngr, cloned in 2001, is a leucine - rich - repeat glycophosphatidylinositol - anchored membrane protein which mediates nogo - 66 inhibition of axonal outgrowth. both the long acidic amino - terminal domain and the nogo - 66 fragment have strong neurite growth inhibitory activity suggest that nogo - a has at least two inhibitory domains. northern blot, in situ hybridization, western blot and immunocytochemistry analyses show that in addition to oligodendrocytes, nogo - a mrna and nogo - a protein are also expressed in neurons in developing and adult brain and spinal cord, nogo - a is also found in peripheral organs such as heart and testis

    Northernblot 、原位雜交、 westernblot和免疫組化結果證明： nogo amrna和nogo蛋白除了在cns的寡突膠質細胞中表達，還表達于發育階段和成年的腦、脊髓和外周神經節的某些神經元中，在外周組織如睪丸和心臟也有表達； nogoe在cns和pns以及多種外周組織中有廣泛分佈； nogo （除表達于腦和心臟外，在骨骼肌中有較高表達。
  3. Northern blot results show that nos. 66 - 1, 84, 89 - 1, 97, 108, 152, 175 and 233 have stronger signal in sp6 - tester than in sp6 - driver ; and no. 23 has weak signal only in sp6 - tester, nos. 94, 165, 172, 185 and 191 have similar hybridization signals in both sp6 - tester and sp6 - driver ; nos. 4, 17, 18, 28, 6 9, 101, 156 - 1, 157 - 1 and 183 do not reveal hybridization signals in both sp6 - tester and sp6 - driver ; the results of sequencing and blastn and blastx on ncbi indicate that no. 23 cdna ( 846bp ) has significant alignments with nicotiana tabacum mrna for elicitor inducible beta - 1 - glucanase nt - sube76, and arabidopsis thaliana clone 7119 for glycosyl hydrolase family 17 ( protein id : at5g55180. 1, supported by cdna : 7119, supported by cdna : gi _ l 87001 54 ) and arabidopsis thaliana beta - 1 - glucanase - like protein ( gi _ 2 1594590 ) ; no. 84 cdna ( 560bp ) has significant alignment with lotus corniculatus aspartate aminotransferase mrna ( complete cds length = 1685, gi | 2605931 | gb | af029898. 1 | af029898 ) for aspartate aminotransferase ; no. 89 - 1 cdna has significant alignment with arabidopsis tha

    與同源性最高的擬南芥類似晚期胚胎發生高豐度蛋白比較，二者都具有lea 2結構域、保守分泌蛋白cog5608結構域和低復雜度區，都具有pkc磷酸化位點、酪蛋白激酶磷酸化位點、 n十四酞化位點和酚胺化位點，所不同的是： （ ）在結構功能域上， 152全長cdna編碼的蛋白質序列中多了1個lea 2結構域、 l個保守分泌蛋白cog5608結構域和1個低復雜度區； （ 2 ）在功能位點上， 152全長cdna編碼的蛋白質具有酪氨酸硫酸化位點、多了l個酪氨酸激酶磷酸化位點和1個可能的天冬氨酸富集區，但沒有n糖基化位點； （ 3 ）擬南芥類似晚期胚胎發生高豐度蛋白的lea 2結構域具有顯著性（ e
  4. Bl21. the dot blot hybridization verified that the scfv was secreted to the medium

    從培養基中可檢測到可溶性抗人絨毛膜促性腺激素的單鏈抗體。
  5. The analysis results of southern blot hybridization among all of specimens are coincidence with the detecting results of pcr products electrophoresis

    所有標本進行southernblot雜交分析，結果予以證實。
  6. Northern blot hybridization showed that leacbf expression had the same tendency with the enzyme activity. and it showed that the transcription factor acbf maybe have some relation with the enzyme activity of pal

    表明在紫外光照射條件下， leacbf編碼的轉錄因子的變化趨勢與苯丙氨酸解氨酶（ pal ）活性的變化趨勢有一定的相關性。
  7. Northern blot hybridization showed that leacbf has well - distributed expression in different tissues of wild tomato ( pink stage ). treatment the seedling with uv made the enzyme activity of the pal increased gradually and then decreased

    用不同的紫外線照射時間處理番茄幼芽，苯丙氨酸解氨酶（ pal ）的活性呈先是增加，達到最大值后再減小的趨勢。
  8. Northern blot hybridization showed that leerfl, leerf2 and pti4 ( a member of erebps in tomato ) expressed differentially in different parts of wild tomato fruit during ripening, which was related with fruit ripening

    Northern雜交分析表明： leerf1 、 leerf2 、 pti4 （已發表的番茄erebps成員）基因在普通番茄果實的成熟過程及果實的不同部位都有差異表達，表現出與成熟進程的相關性。
  9. Lastly by using the technique of dot blot hybridization, the genome dna of chlamydia was detected with the probe of momp gene labeled with dig - 11 - dutp by using the way of random primer. the results showed the degree of sensitivity of the probe was 10 pg and other pathogens could not be detected by this probe. by comparing the diagnostic ways of nucleotide probe and fc, the technique of nucleotide probe were proved to have high sensitivity and speci fi city

    最後，用地高辛隨機引物法標記成momp基因核酸探針，斑點雜交檢測衣原體基因組dna ，靈敏度可達10pg ，且不能檢出其它病原體的核酸。將核酸探針法與補體結合反應法對衣原體感染的診斷進行比較，初步證明該探針具有較高的敏感性與較強的特異性。
  10. Fingerprints of 5 strains of the inbred mice and 2 strains of the inbred rats was conducted using a nonisotopically hrp labeled jl - 02 by the second institute of the public safety bureau of china and southern blot hybridization, the author studied many fingerprints of the same dna, the different organic fingerprints of the same organism and fingerprints of parent and offspring. the patterns were completely different among the different strains and those of the samples from the same strain were completely identical

    採用公安部二所自行研製的jl - 02多位點探針對5個品系的近交系小鼠和2個品系近交系大鼠進行了dna指紋分析，經過對同一dna的反復製作dna指紋圖和同一個體不同組織進行的dna指紋圖製作及對親代和子代（同品系內和不同品系間雜交）間的dna指紋圖比較。
  11. Hybridization bands were detected by southern blot analysis using lea3 gene probe labeled by digoxigenin the result showed that foreign lea3 gene integrated into strawberry genome

    2 。對點雜交為陽性的植株進行southernblot分析，轉化植株檢測到了雜交帶，除預期的1
  12. Was multiplied and the tk gene was cloned. the cloned tk gene was retrieved by proper restrictive hemodynamics. the retrieved tk gene was labeled by digoxin according to the kit of labeling and detection of digoxin. then, the specificity and sensitivity of tk gene probe were detected with dot blot hybridization. the sequence of tk gene of nm98a strain was analysed. the result of the analysis of tk gene ' s sequence confirmed that autoploidy between tk gene of nm - 98a strain and issued strain was 99. 7 %

    本研究中首次對iltv - nm98a株的tk基因進行了克隆和序列分析，結果表明： iltv - nm98a株tk基因的核苷酸序列與已發表的iltvtk基因的核苷酸序列具有高度的同源性，兩者之間僅相差4個核苷酸，同源性高達99 . 7 ，從而證實了iltvtk基因是高度保守的，為iltvtk基因核酸探針的制備提供了有力的依據。
  13. Protocol of dot blot and in situ hybridization for white spot virus

    對蝦白斑病毒斑點雜交和原位雜交檢測操作規程
  14. Was first processed by dgd embedment and embedment - free technique and general technique for em morphology. a perinuclear structure consisted of interacted filaments we called lamina - like structure was observed. then using western blot assay, we found a lamin - like component band of 68kd protein in the three - step - fractionated cells. to investigate the distribution of the lamin - like protein in cells, a immunofluorescence experiment for in situ hybridization was designed using goat anti - lamin protein antibodies as the probes. the results revealed that the positive reactivity presented at different part of the cells. the perinuclear cross - actions were distinct, and cross - action with the oral apparatus and the cortex were also obtained

    本文以dgd包埋去包埋技術對草履蟲的核纖層通過透射電鏡和免疫熒光分子雜交等技術進行了觀察。結果顯示，在核周存在由10nm纖維組成的核纖層免疫熒光結果表明，在核周呈陽性反應，並在其表皮口器等部位呈交叉的陽性反應蛋白分子雜交的反應帶在68kd處呈陽性反應。
  15. We further performed northern blot hybridization to reveal the regional distribution of 3galt - l transcripts in mouse brain. in cerebral cortex, p3galt - l was expressed abundantly in newborn mice with a slight decrease in adult mice, and a similar expression pattern could also be detected in hippocampus

    我們發現：在大腦皮質和海馬中， 3galt - 1的表達水平在發育進程中都相對較高；在間腦和腦干中， 3galt - 1的表達水平則較低；而在小腦中， 3galt - 1在新生鼠中表達很高而在成年鼠中表達明顯下降。
  16. 61 of these clones have hybridization signals on the x - photos of the reverse northern blot. then northern blots were performed for 23 of these 61 clones : nos. 4, 17, 18, 23, 28, 66 - 1, 69, 84, 89 - 1, 94, 97, 101, 108, 152, 156 - 1, 157 - 1, 165, 172, 175, 183, 185, 191 and 233

    其結構功能域中包含1個28個氨基酸殘基的信號肽ol2個晚期胚胎發生高豐度蛋白lea毛結構域（ 56 206 ， 205 323 ） 、 2個保守分泌蛋白cog5608結構域（ 72 200 ， 174 281 ）和3個低復雜度區門， 4265 ， 329習44其h級結構中存在有大量的捲曲螺旋區。
  17. I and hindlll respectively. after the digested products were run via agarose gel electrophoresis and transferred into nylon membrane, the southern blot was carried out using the cdna of rubber tree etrl as probe. the result of the southern blot showed that a hybridization band ( - 3. 0kb ) turned up from the ecor i digested product and another band ( - 4. 8kb ) turned up from the hindi ]

    從橡膠樹pr107品系的嫩葉提取基因組dna ，用限制性內切酶ecor 、 hinofll分別酶切，瓊脂糖凝膠電泳分離並轉膜后，用克隆的橡膠樹etri基因的cdna片段作探針進行southern雜交分析，結果表明， ecor酶切在約3 okb處有一條雜交帶出現， hi 。
  18. Methods : extracting the total rna of human pbmc, the objective include 60 healthy blood donator, 30 patient with viral encephalitis and multiple sclerosis and parkinsonian syndrome, 30 patient with schizophrenia and affective disorder, this indviuals were inpatients or outpatients of the first hospital of chongqing university of medical science from december, 2000 to june, 2001. using nested rt - pcr techique to detect borna disease virus " middle fragment in orf i, and using southern blot hybridization to analyze the pcr product

    重慶醫科大學碩士學位論文方法：提取從2000年12月至2001年6月在重慶醫科大學第一附屬醫院神經科和精神科住院及門診的60例健康獻血者、 30例包括原因未明的病毒性腦炎、多發性硬化、帕金森綜合征，以及30例包括精神分裂癥和情感障礙患者pbmc中的總kn 』 a ，採用套式逆轉錄聚合酶鏈反應estedrticr ）技術進行了bdvorf基因中部片段的檢測，並對pcr產物進行電泳分析及southernblot雜交分析。
  19. Twentymo transgenic plants were selected by dna dot blot analysis, 15 of them detected hybridization signals and the positive rate was 68. 2 %

    通過dnadotblot分析，對22個轉化株進行篩選，其中15株檢測到雜交斑，陽性率為68
  20. Halobacillus trueperi accumulates glycine betaine under condition of high osmolarity. a partial fragment of the glycine betaine transporter beth gene was obtained from the genome of h, trueperi with degenerate primers. through southern blot hybridization and inverse pcr, a 5. 1 kb ecori fragment containing the beth gene was sequenced

    將擴增片段用地高辛標記成探針，與用不同限制性內切酶完全酶切的h . trueperi總dna片段作southern雜交，結果顯示在ecori酶切片段的5 . 1kb處有陽性信號。
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