brdu 中文意思是什麼
brdu
解釋
5-溴脫氧尿核苷,溴脫氧尿苷,溴苷-
2 - e4 and s2 is induced respectively by 8 - ag and 5 - brdu with different drug concentration to make them deficient in hypoxanthine - guanine phosphoribosyl transferase ( hgprt ) and in thymidine kinase ( tk ) respectively and renamed 2 - e4 - a and 82 - 6. their antibodies " isotypes are tested by goad anti - mouse isotype regent
取馴化好並處于對數生長期的2 - e _ 4 - a和s _ 2 - b細胞，再常規融合和篩選，三次克隆化后得穩定分泌雙特異性抗體的雜交-雜交瘤細胞株6株。 -
When cultured in vitro for 8 days, nscs were divided into three groups : the first one is the group to identify and evaluate nscs " property and proliferating capability ; the second one is the group to induce differentiation of nscs by egf, bfgf, 1 7 - estrodiol and atra respectively ; the third one is the group to investigate the toxicity of a p 25 - 35 and protective action of 1 7 - estrodiol against a. the above - mentioned groups were cultured continuely according to formerly conditions, then conducting the following experiments : 1. identification of nscs and evaluation of their proliferating capability brdu ( 5mol / l ) was added into the first group to label cells when group division was conducting
3 . 3ap25一35和ap25一35 +雌激素作用后凋亡相關基因bcl一2 ， bax表達鄭州大學2003年碩七研究生畢業論文ap25 - 35對胚胎神經卜細胞的毒性和雌激素的保護作用對照處理組bel一2 、 bax均無表達;雌激素保護組bel一2及bax均有表達; ap毒性組僅有促凋亡基因bax表達，而無凋亡抑制基因be卜2表達。 -
Methods for qualitative and quantitative characterization of primmorph culture were developed, which included a modified mtt assay for measurement of cell viability and cell growth, a 5 - brdu - incorporation assay for detection of cell proliferation, propagation and metabolism of in vitro primmorph
系統考察並優化了mtt法應用於海綿細胞培養過程細胞活性和細胞生物量定量的條件，結合5 - brdu摻入法建立了細胞團培養過程中細胞增殖能力和細胞活性的評價方法。 -
And further studied e rosettes experiment and lymphocyte proliferation brdu - elisa assay
並對初步篩選的e玫瑰花環實驗和淋巴細胞增殖brdu - elisa實驗進行了進一步篩選。 -
Finally, we ensure immunobiologic activity evaluation methods of gpif wre t cells from pigs thymus of e rosettes experiment and pha inducing lymphocyte proliferation brdu - elisa assay
最終確定gpif免疫生物活性評價方法為： t細胞來源於胸腺的e玫瑰花環實驗和pha誘導的淋巴細胞增殖brdu - elisa實驗。 -
3. the choosing of optimum evaluating methods of immunobiologic activity : if we disposed sheep blood red cells with neuraminic acid enzyme, the e rosettes forming ratio remarkbly increased ; we improved the conventional method of brdu - elisa assay on lymphocyte proliferation induced by cona. the results showed that lymphocyte proliferation induced by pha was better than by cona. 4
3 、 gpif免疫生物活性評價方法的優化對gpife玫瑰花環實驗在不影響srbc活性的基礎上用神經氨酸酶處理，經此法處理后e玫瑰花環形成率明顯提高；對傳統的cona誘導的人外周血淋巴細胞增殖brdu - elisa實驗，進行了方法學改進。 -
2. the comparison of methods of immunobiologic activity evaluation : in this paper, we primitivily selected two kinds of morphologic methods to evaluate gpif immunobiologic activity : e rosettes experiment, lymphocyte transformation experiment, and two kinds of optic colorimetry immunobiologic activity evaluation methods : cell proliferation mtt assay, cell proliferation brdu - elisa assay
2 、 gpif免疫生物活性評價方法的比較篩選初步比較篩選了兩種形態學免疫生物活性評價方法， e玫瑰花環實驗和淋巴細胞轉化實驗及兩種光學比色免疫生物活性評價方法，淋巴細胞增殖實驗mtt法和淋巴細胞增殖實驗brdu - elisa法。 -
M ethods we successfully expanded human embryonic brain - derived nsc into spheres with mitogens. the nsc were identified by immunocytochemistry method, brdu labeling and cell cloning were used to observe the proliferation ability of nsc. pdgf x t3 were separately used to induce the differetiation of nsc
方法：本實驗以胎齡為10 - 12周的人大腦皮質為材料，在體外成功誘導擴增nsc ，用免疫細胞化學方法鑒定nsc ，用brdu標記和細胞克隆分析觀察了nsc的增殖能力。 -
We detected the transcription and translation change of different thrs genes by semiquantitative reverse transcription - polymerse chain reaction ( rt - pcr ) analysisjelisa respectively. at same time the differentiated cell were identified by immunocytochemistry method. results ( l ) nsc induced by mitogens were nestin - positive and incorporated by brdu
以pdgf 、 t _ 3對nsc進行誘導分化，並分別用rt - pcr半定量法、 elisa法檢測nsc在不同誘導因子作用下分化前後thr各亞型在轉錄與翻譯水平上的表達變化，用免疫細胞化學方法鑒定分化后的細胞類型。 -
3 after incubated with 5 - bromo - 2 - deoxyuridine ( brdu ) for 7 days, newborn cells derived from regenerative neurospheres were immunostained. 4 the cells were collected by centrifugation separation and put into poly - 1 - lysine - coated terasaki wells, which were randomly divided into one of three groups of 24 wells cells each
4將傳代培養的神經球離心收集，轉移至24孔培養板，隨機分為3組，每組24孔細胞，置5 % coz 、 370c培養箱，連續培養7d ，觀察神經球的誘導分化。 -
Two days later ( that is, these cells had been cultured for ten days in vitro from isolation ) these cells were collected and immunocytochemistry was performed by anti - nestin to identify nscs and by anti - brdu to evaluate nscs " proliferating capability
3 . 4ap25一35和ap25一35 +雌激素作用后細胞增殖能力檢測對照組神經幹細胞brdu陽性細胞率約為50 % ;雌激素保護組及ap毒性組brdu幾乎未見陽性細胞。 -
2 after incubated with brdu for 7 days, the newborn cells became brdu - positive
Pbs替代雜交液組的細胞未見gm一csfmrna陽性信號。
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