c terminal 中文意思是什麼
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The fusion protein was bactericidal active against staphylococcus aureus. in present study, we will truncate the none channel - forming do main, then attach the agrd to the pore - forming region ( k544 - i626 ) to construct a new engineered multidqmain protein machine - compact engineered peptide targeting staphylococcus aureus. such engineered peptide was constructed by linking the gene of staphylococcal agrd pheromone with the gene of c - terminal ( 1626 ) of colicin la pore - forming region ( k544 - i626 ) with site - directed mutation
利用點突變方法將金黃色葡萄球菌信息素agrd ( i型, ystcdfim )的基因引入到大腸菌素fa梭基端1626基因上,並將限制性內切酶sacl酶切位點基因分別引入到大腸菌素fa的p4和k544上,通過酶切、膠回收、連接獲得含大腸菌素ia水性孔道結構域和金黃色葡萄球菌信息素agrd基因的重組質粒。 -
In the 1990s, the pheromones of gram - positive bacteria, which regulates the growth and toxin secretion of the same type bacteria, were identified they were peptides consisted by dozens of amino acids. the pheromones can auto - recognize membrane receptor of the identical types of bacteria. we had constructed a fusion protein named pheromonicin by introducing a staphylococcal pheromone agrd at the c - terminal of the colicin ia
丘小慶等利用信息素的自主導向特性和大腸菌素ia的致死性通道特性構建了由金黃色葡萄球菌信息素agrd和大腸菌素ia組成的融合蛋白,命名為pheromonicin ,該蛋白表現出了信息素和大腸菌素ia都不具有的抗金黃色葡萄球菌活性。 -
Homologues of era have been identified in prokaryotes and eukaryotes. there are two domains in era : the n - terminal gtp - binding domain and c - terminal rna binding kh domain. era plays an important role in cell cycle progression at a specific point in the cycle, after chromosome partitioning but before cytokinesis
Era是在原核和真核生物以及植物中普遍存在的g蛋白,是一個結構上既具有gtp結合結構域又有rna結合結構域、不同於ras的獨特的新的g蛋白亞家族。 -
The results show that : four histidine derivatives from c - terminal were synthesized which is based on the forming peptide bonds, and obtained 58 - 73 % of compounds from boc - his ( tos ) - oh
實驗結果表明:採用多肽合成的方法得到了4個組氨酸羧基末端的衍生物,而且產率較高( 58 73 ) 。 -
In particular, the resulting active ester with pentafluorophenol facilitated the subsequent reaction with an amine and the hydrazine derivative to yield the c - terminal amide and hydrazide, respectively
特別是與五氟苯酚生成的酯對后續的與氨或肼衍生化分別生成c端酰胺或酰肼非常有利。 -
It is most efficient in clearing the peptide bonds on the c-terminal side of such amino acids tyrosine.
它對裂開諸如酪氨酸這類氨基酸的羧基末端的側肽鏈最為有效。 -
The enzyme digest analysis shows that the arm repeats of c - terminal are conceivably conservative domain. in arc1 protein, there are some active sites including n - glycosylation sites, camp - and cgmp - dependent protein kinase phosphorylation sites, protein kinase c phosphorylation sites, casein kinase ii phosphorylation sites, tyrosine kinase phosphorylation sites, n - myristoylation sites, amidation sites and leucine zipper pattern. it probably take part in the signaling process of self - incompatibility
同時在arc1蛋白質中還發現了拉鏈結構和多個磷酸化位點,包括camp和cgmp依賴的蛋白激酶磷酸化位點、蛋白激酶c磷酸化位點、酪蛋白激酶磷酸化位點、酪氨酸激酶磷酸化位點、糖基化位點等,拉鏈結構為arc1蛋白之間及與其它蛋白的相互作用提供了可能,而磷酸化位點是arc1參與信號傳導過程所必需的。 -
A decapeptide ( nh2 - nyqkdalgfl - cooh ) and a hexadecapeptide ( nh2 - kakesdagflmfvylv - cooh ) were synthesized as model peptides, then covalently attached to ditc glass beads for methodology study in tbus - itc and tpge - itc chemistry. four c - terminal residues of the synthetic decapeptide have been identified
利用偶聯到川tc玻璃珠上的10膚,對tpge一1tc作偶聯試劑的活化劑用量、活化時間、偶聯溫度和偶聯時間進行了優化,確定了最佳反應條件。 -
We fused the gfp into the c - terminal region as well as n - terminal region of emt - 1 protein. the result showed that the localization of the protein encoded by the full length emt - 1 cdna was in endoplastic reticulum ( er ). extracellular region, transmembrane and intracellular region showed the similar cellular localization
我們用綠色熒光蛋白融合於emt l全長及其不同截斷形式的梭基和氨基端,瞬時轉染cos 7細胞,通過熒光顯微鏡觀察,發現emt l編碼的蛋白呈現內質網定位的特點。 -
The generation of endostatin is catalyzed by proteolytic enzymes, that cleave peptide bonds within the protease - sensitive hinge region of the c - terminal domain
特異性蛋白水解酶降解膠原c末端的蛋白酶敏感區,產生內皮抑素。 -
In vivo, the two functional forms of rat mapllc3 with apparent mobilities of 18 and 16kda, termed lc3 - i and lc3 - ii, separately, were produced by a series of post - translational modifications including a characteristic c - terminal cleavage after the conserved glyl20 residue and this cleavage is essential for the membrane association of the 16kda rat map1lc3 protein.,
其中18kda的map1lc3蛋白是微管相關蛋白1的輕鏈亞基,稱為lc3 - ;另一種16kda的map1lc3蛋白則是自噬體膜的必須組分,稱為lc3 - 。 lc3 -和lc3 -都是一系列翻譯后修飾的產物,其中特徵性的修飾是在保守gly120后發生的羧基端切割,此切割修飾對lc3 -定位於自噬體膜尤為重要。 -
In vivo, the two functional forms of rat map1lc3 with apparent mobilities of 18 and 16kb, termed lc3 - i and lc3 - ii, separately, were produced by a series of post - translational modifications including a characteristic c - terminal cleavage after the conserved gly120 residue and this cleavage was essential for the membrane association of the 16kd rat mapllc3 protein
Lc3 ?和lc3 ?都是一系列翻譯后修飾的產物,其中特徵性的修飾是在保守gly120后發生的羧基端切割,此切割修飾對lc3 -定位於自噬體膜尤為重要。我們研究發現在大鼠,小鼠和人中map1lc3主要以兩種型存在, lc3a型和lc3b型。 -
An unusual rice calmodulin isoform, oscam61, was first obtained in our lab, which contains an n - terminal cam domain and a c - terminal basic extension with a potential prenylation site. in vitro activity assays confirm oscam61 as a functional calmodulin. using the green fluorescent protein ( gfp ) as a visual marker, we further studied subcellular localization of oscam61 in stably transformed tobacco cells
利用綠色熒光蛋白( greenfluorescentprotein , gfp )作為標記,研究了oscam61在煙草細胞中的定位, gfp - oscam61融合蛋白(具有開放的異戊烯化修飾位點)定位於細胞質膜和細胞器膜上,而oscam61 - gfp (異戊烯化修飾位點被gfp封閉)定位於細胞核的核質中。 -
The ir sequence of mxmybl is most homologous with that of atmyb ( 86. 9 % ) and somewhat homologous with that of stmyb 1 ( 77. 0 % ) ; there is very low homology among n - and c - terminal regions outside of the ir regions of all of the mybs ; the protein mxmybl contains a proline - rich region as well as a serine - rich region near the c - terminus, such structure motifs are implicated in transcriptional activation
9 ,與馬鈴薯stmyb的ir序列的同源性達77 0 ,所有這些nnrb蛋白除了瓜區具有較高的同源性外,其c端和n端幾乎沒有同源性。 mwyb蛋白的c一端還含有一個富含脯氨酸區,這樣的結構基序可能具有激活轉錄的功能。 -
It contained 187 amino acids deduced from the nucleotide sequence, and showed 65 % amino acid sequence identity with human aoeb166. the analysis of n - and c - terminal domains revealed amino acid sequences characteristic of features of mitochondrial and peroxisomal targetin
原位雜交分析表明從受精卵到神經胚, aophihmgb基因廣泛表達,但隨著發育的進行,中胚層、內胚層和神經管的表達減弱,到1天幼蟲期,僅在表皮可檢測到表達。 -
The predicted vfcpkl protein with 493 amino acids contains all the structural characteristics of reported cdpks and there are four domains of a variable domain, a kinase domain, an autoinhibitory domain and a calmodulin - like domain from n - to c - terminal end
由編碼區推測的vfcpk1的493個氨基酸序列具有已報道的cdpk的所有典型結構特徵,由n端到c端可分為可變區、激酶區、連接區和調節區四個結構域。 -
A pair of primers, based on the high homologous n - and c - terminal amino acids sequences of subtilisin of bacillus pumilus, were designed and used to amplify subtilisin gene from genomic dna of 4 different bacillus strains by polymerase chain reaction
Sds 、 edta和pmsf對酶活力有不同程度的抑制作用。以4株芽孢桿菌的總dna為模版,利用擴增短小芽孢桿菌堿性蛋白酶基因的引物對它們進行pcr擴增。 -
Structure analysis indicated that the molecule of hwtx - i consists of a small triple stranded anti - parallel ( 3 - sheet and five ( 3 - turns. the n - terminal, c - terminal and most basic amino acid residues are located in the surface of the molecule
構象研究表明, hwtx -由一小段三股反平行的-折疊和5個-轉角組成,肽鏈的n端和c端以及多數堿性氨基酸殘基都分佈在分子表面。 -
Following researches verified that the cam - binding domain exists in the c - terminal of ecbp21
通過缺失表達分析實驗證實cambd結構域位於ecbp21的羧基端。 -
The complete human znf325 cdna sequence is 2697bp and contains a 1389 - bp open reading frame ( orf ) that encodes a 462 amino acid protein with 15 zinc finger c2h2 motifs. the complete human znf359 cdna sequence is 3270bp and contains a 1932 - bp open read ing frame ( orf ) that encodes a 643 amino acid protein with an n - terminal krab domain and 16 c - terminus zinc finger c2h2 motifs. the zfp28 cdna sequence is 4104bp and contains a 2076 - bp open reading frame ( orf ) that encodes an 868 amino acid protein with an n - terminal signal peptide, two krab domains and 14 c - terminal c2h2 zinc finger motifs
Znf325長2697個堿基,含有一個長為1389個堿基的開放閱讀框,編碼一個長為462個氨基酸的蛋白質,它包含15個c2h2型鋅指結構; znf359長3270個堿基,含有一個長為1932個堿基的開放閱讀框,編碼一個長為643個氨基酸的蛋白質,它包含一個n -端krab結構域和16個c -端c2h2型鋅指結構; zfp28長4104個堿基,含有一個長為2076個堿基的開放閱讀框,編碼一個長為868個氨基酸的蛋白質,它包含一個n -端信號肽,兩個krab結構域和14個c -端c2h2型鋅指結構。
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