carboxy terminal 中文意思是什麼
carboxy terminal
解釋
羧端-
The three isoforms have a common carboxy - terminal domain of 188 amino acids, and this region is highly homologous to the reticulon protein family
在nogo分子的c -末端，有兩個跨膜片段，被66個氨基酸殘基組成的環狀結構（ nogo - 66 ）所分隔。 -
Many glycoproteins of lower and higher eucaryotes are attached to the plasma membrane by means of a glycosylphosphatidylinositol ( gpi ). gpi - anchored proteins are synthesized on membrane - bound ribosomes. upon translocation of the pro - protein across the endoplasmic reticulum membrane, gpi : protein transamidase ( gp1t ) recognize and removes the carboxy terminal gpi signal sequence and attaches a gpi molecule to the newly exposed carboxy terminal amino acid
Gpi化前體蛋白在依附於膜的核糖體上合成，當其易位穿過內質網（ er ）膜后，被gpi ：蛋白質轉酰胺基酶（ gpit ）識別， gpit在移走其羧基端gpi信號序列的同時將gpi分子連接至新生成的氨基酸位點上。 -
Hegf gene with his - tag at the end, which was derived from pet22 - egf, was in - frame fused to the carboxy - terminal of polyhedrin ( ph ) gene, which included the amino - terminal 116aa coding region. the polyhedrin - egf fusion gene ( named ph - egf ) was then cut out with ecorv and ecori, and was cloned between ecorv and ecori sites of pbacpak. 8 ( the result plasmid was named pbacph - egf and the ph - egf fusing gene was right under the control of ph promoter ). the pbacph - egf structure was verified with restriction enzymes digestion and pcr
從pet22 - egf質粒中分離出末端帶his - tag的egf基因，對位融合於多角體蛋白n端116個氨基酸基因序列的下游（命名為ph - egf ） ，並在兩段基因間設計了凝血酶xa蛋白酶切位點，經過酶切、測序等鑒定正確后，克隆至pbacpak8中，使ph - egf融合基因置於多角體蛋白（ polyhedrin ， ph ）基因啟動子控制之下，構建成重組轉移載體pbacph - egf 。
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