cationic protein 中文意思是什麼
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Differetial diagnostic value of eosinophil cationic protein in children with cough
嗜酸細胞陽離子蛋白對咳嗽患兒鑒別診斷的價值 -
The study of the relationships between eosinophil cationic protein and corticosteroid in asthma with acute attack
糖皮質激素對支氣管哮喘急性發作期患者血清嗜酸細胞陽離子蛋白及肺功能的影響 -
Expression in vitro cos - 7 cells were transfected with pm, pms, pmi and pmsi constructs by cationic liposom, respectively. 48 hours later, mrna of targets gene were detected by rt - pcr and hil - 12 protein in culture supernatnant and cell lysates were detected by western blotting. p815 cells were transfected with pm and pms constructs and selected by g418
2 .重組質粒在真核細胞中的表達: （ 1 ） pm 、 pms轉染cos一7細胞， 48小時后，用ri 』 - pcr檢測目的基因在mrna水平的表達;轉染p815細胞， g418篩選抗性細胞克隆，用rt - pcr檢測目的基因在mrna水平的表達，結果為陽性，說明在轉錄水平有目的基因的表達。 -
In order to study immunization of genetic vaccine by inoculation, two kinds of dna vaccine of ndv are constructed and analysed their sequense, which will express f protein in eukaryotic cell ( hela cell ) in vitro with the method of transfection by cationic liposome the results testify that two sorts of plasmids dna successfully expressed the f protein of ndv in hela cell, but the expressed content of pc4. 0f is higher than ones of pc3. 1f
將構建的基因疫苗pc3 . 1f大量提取和純化，採取肌肉注射和腹腔注射兩種途徑、不同劑量免疫小鼠，用elisa方法檢測小鼠血清中抗f基因表達產物的抗體水平。研究結果表明，基因疫苗pc4 . 0f和pc3 . 1f均在hela細胞中成功表達，其中pc4 . 0f的表達量比pc3 . 1f高。
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