cell culture in vitro 中文意思是什麼

cell culture in vitro 解釋
離體細胞培養
  • cell : n 1 小室，單室；隔間，艙；〈詩〉茅舍；（單個的）蜂窩，蜂房。2 〈詩〉墓穴，墓。3 （大修道院附屬的...
  • culture : n. 1. 教養；修養；磨煉。2. 文化，（精神）文明。3. 人工培養，養殖；培養菌，培養組織。4. 耕作；栽培；造林。vt. 使有教養。
  • in : adv 1 朝里，向內，在內。 A coat with a furry side in有皮裡子的外衣。 Come in please 請進來。 The ...
  • vitro : 比特羅
  1. According to the mechanism of block of development in vitro culture on early embryo of mammal and in vivo surroundings of early embryo, the paper states that requirement and utilization of nutrients during each cell stage of early embryo of mammal in vitro culture in order to search for in vitro culture condition and method to improve the development rate of blastosphere

    摘要從哺乳動物早期胚胎體外培養發育阻斷機理和早期胚胎的體內環境入手，闡述了胚胎體外培養各細胞階段胚胎對營養物質的需求，尋求合理的體外培養條件和方法，以便提高體外胚胎早期的囊胚發育率。
  2. Spontaneous differentiation of es cell d3 line in vitro es cell d3 line via suspension culture can congregate small mass within 24 h, and large numbers of aggregates called embryoid bodies ( ebs ) gradually formed after 2 to 3 days of culture

    5 . es一d3細胞系的自主分化es一d3細胞懸浮培養， 24h后可聚集成小的細胞團，第2 ~ 3d時形成大量的類胚體（ ebs ） 。
  3. There is no different between the fibroblast feeder of mouse and the fibroblast feeder of goat according to blastoysts attached and icm proliferation. es - like cell develop embryoid body - like structure in suspension culture in vitro. es - like cells of goat differentiate into various kinds cell, egg : fibroblast cell, neurolike cell

    Es細胞在體外懸浮培養，形成類胚體，山羊類es細胞在無飼養層鋪有明膠的皿底上培養，可分化為多種類型細胞，如上皮細胞、成纖維細胞、神經細胞等。
  4. Thus, in order to investigate the developmental pathways not only involved in the regulation of growth and patterning, but also in the determination of cell lineages and differentiation, we utilized the fluorescent immunohistochemical methods, flow cytometry analysis sorting ( facs ) and molecular methods to investigate the developmental law of mammary gland at the different developmental stages, distribution of the stem cells in mammary gland, the methods of isolation, culture and evaluation for the stem cells, the multipotent abilities in vivo and in vitro, and the efficient cultural system for stem cells enriched in vitro. the results showed below : 1

    我們以小鼠為模型，運用組織化學、免疫熒光組織（細胞）化學、流式細胞儀分選方法（ facs ）以及分子生物學手段，研究了小鼠乳腺的發育規律：小鼠乳腺組織中類乳腺幹細胞：小鼠乳腺細胞的分離、培養以及類乳腺幹細胞的鑒定；小鼠類乳腺幹細胞分化的潛能；小鼠乳腺類腺體體外短期培養富集類乳腺幹細胞體系的優化等。研究結果表明： 1
  5. Fibroblast cell of adult mouse culture in vitro

    成年小鼠成纖維細胞體外培養
  6. Part 1 : the culture and identification of es - d3 cells and the study of the efficiency of eb formation from es cells when grown on mef feeder layer in es culture medium or cultured in es culture medium supplemented with lif 1000u / ml, es - d3 cells being used in our experiments formed normal clones, expressed akp and kept their normal karyotype over many passages. the in vitro and in vivo differentiation experiments showed that es - d3 cells could differentiate into variety of cell types derived from three primary germ layers

    結果顯示： eso3細胞在小鼠胚胎成纖維細胞上和或含白血病抑制因於億f ）的es細胞培養液中形成典型的胚胎幹細胞克隆，堿性磷酸酶染色結果為強陽性，具有正常二倍體核型以及具有在體內外分化為三個胚層來源的組織細胞的潛能，而且具有形成種系嵌合動物的能力。
  7. Endothelial cell culture in vitro from umbilical blood

    臍血內皮細胞的體外培養
  8. In the protein adsorption and osteoblast culture in vitro, more surface hydroxyl groups and higher polar component of surface energy led to more protein and cell adsorbed, and higher cellular activity

    表面羥基，包括堿性羥基和酸性羥基含量越高，表面能的極性分量越大，吸附的氧化欽膜、含鈣和丈磷的認表面表徵與認生物沽件蛋白質和細胞越多，細胞活性越高。
  9. An infectious eiav clone was recovered by transfecting fatal donkey dermal ( fdd ) cell cultures and donkey leukocyte ( dl ) culture in vitro with the full - length gene clone of dv. the virus ( designed pd70344v ) derived from the third passage in dl culture was observed by electron microscope and the reverse transcriptase ( rt ) activity was determined

    將包含全基因片段的三個基因克隆以限制性內切酶消化后順次連接克隆到載體ptz18r上，構建了4個全長基因的分子克隆，分別命名為pd30343 、 pd70333 、 pd70343 、 pd70344 ，其中兩個轉移到低拷貝載體plg338上，命名為plgd30343 、 plgd70344 。
  10. Methods for qualitative and quantitative characterization of primmorph culture were developed, which included a modified mtt assay for measurement of cell viability and cell growth, a 5 - brdu - incorporation assay for detection of cell proliferation, propagation and metabolism of in vitro primmorph

    系統考察並優化了mtt法應用於海綿細胞培養過程細胞活性和細胞生物量定量的條件，結合5 - brdu摻入法建立了細胞團培養過程中細胞增殖能力和細胞活性的評價方法。
  11. In this paper, the whole process of it microsporogenesis and male gametophytes development was observed with microscope to sure weather stamen development is normal. at the same time, in order to provide techniques on biotechnology conservation and the foundation of its resources gene pool in cell engineering, its techniques on culture in vitro was studied

    本論文通過對蝟實小孢子發生和雄配子體發育全過程進行細胞觀察，探尋蝟實的雄性器官的發育是否是蝟實有性生殖的薄弱環節，並對蝟實的離體培養進行了初步的研究，為蝟實生物技術保存、建立蝟實種質資源基因庫提供細胞工程方面的途徑和技術。
  12. A rotary cell culture system was designed, which was composed of micromotor, rotate part, gear part and et al. in this system, tubular scaffold can rotate slowly around own major axis. by means of this, three - dimension tissues or organs can be cultured in vitro

    我們還特別設計了旋轉培養裝置，通過微型馬達驅動，減速裝置作用使旋轉部分繞自身中心水平軸做緩慢轉動，從而使置於旋轉部件上的管形支架繞其正中長軸作緩慢旋轉，其原理與美國genzrpe公司的rccs （ rotarycellculturesystemrccs ）系統相仿。
  13. The aims of this work are to study the mechanisms for cell aggregation, division / propagation, and metabolism in in vitro sponge primmorph culture system ; and to further optimize the primmorph culture toward the establishment of a continuous sponge cell line. primmorph cultures from mcp were established using several marine sponges species, collected from china oceans and mediterranean sea

    本論文圍繞海綿細胞離體穩定培養體系的建立，對海綿細胞離體培養的技術和規律進行系統的研究和優化，提出了一種新的富集海綿中全能細胞原細胞成團培養的技術adcp - primmorph （ primmorphfromarchaeocyte - dominant - cellpopulation ） 。
  14. By this method of primary culture, cells and tissues could not only subsist for 11 months and more in vitro, but also be subcultured. these works lay a foundation of the establishment of silkworm embryonic cell line

    為建立新的家蠶胚胎細胞系奠定了一定的基礎。原代培養的細胞和組織塊在體外有一定的生長現象，細胞體外最長存活達門個月以上。
  15. Expression in vitro cos - 7 cells were transfected with pm, pms, pmi and pmsi constructs by cationic liposom, respectively. 48 hours later, mrna of targets gene were detected by rt - pcr and hil - 12 protein in culture supernatnant and cell lysates were detected by western blotting. p815 cells were transfected with pm and pms constructs and selected by g418

    2 .重組質粒在真核細胞中的表達: （ 1 ） pm 、 pms轉染cos一7細胞， 48小時后，用ri 』 - pcr檢測目的基因在mrna水平的表達;轉染p815細胞， g418篩選抗性細胞克隆，用rt - pcr檢測目的基因在mrna水平的表達，結果為陽性，說明在轉錄水平有目的基因的表達。
  16. The stage when the block occurs has been correlated to the time the embryonic genome takes over control of development from the maternal genome, which occurs in mouse at the 2 - cell stage. the embryos of balb / cxicr fl mouse show 2 - cell block in vitro and the effects of several culture media on overcoming the 2 - cell block in embryos development were studied. m16 ( sigma ), in which 96. 2 % of 1 - cell embryos developed to 4 - cell stage, were shown to be effective in overcoming the 2 - cell block

    培養5h后， 53去核卵母細胞可見標記的mtocs ，但數量明顯少於對照組，而又多於培養0h的去核細胞，說明此時53的化學去核卵胞質中確有mtocs的存在，其微管聚合能力正在恢復； mtocs標記，但其輻射產生的微管纖維短，結構還不夠豐滿；其餘細胞的微管蛋白則喪失重新裝配能力。
  17. We applied single cell gel electrophoresis and cell culture technique, which constitute sing cell gel electrophoresis assay system for detecting mutagenicity to detect mutagenesis in vitro induced by animal drug quinocetone and olaquindox. and confirmed optimum lysing - time. vero cells in the period of logarithm - growth time were treated with 9. 1 ~ 273u mol / l h2o2 at 37 3h, then were lysed for lh, 2h, 3h and 4h to find optimum lysing - time

    並基於陽性致突變物h _ 2o _ 2作用於非洲綠猴腎vero細胞引起細胞dna損傷的原理，研究了其關鍵步驟-裂解時間，以9 . 1 273 mol l劑量的h _ 2o _ 2染毒處于對數生長期的vero細胞3h后，收獲細胞用於制備三明治凝膠板，分別裂解1h 、 2h 、 3h和4h並選擇最適裂解時間。
  18. The development of a growth medium is one of the essential prerequisites for sponge cells grown in vitro. the mtt ( 3 - ( 4, 5 - dimethylthiazol - 2 - yl ) - 2, 5 - diphenyltetrazolium bromide ) assay was adapted to screen potential nutritional factors in formulating a growth medium for a primary cell culture of suberites domuncula

    Adcp一pri ~ orph培養具有與mcp一pri ~ orph顯著不同的成團過程，增殖細胞比例提高了近3倍，細胞最大生長達到接種時的4倍，明顯優于mcp一pri ~ orph培養。
  19. Embryonic stem ( es ) cells are pluripotent cells derived from the inner cell mass ( icm ) of early blastrocyts, which possess the ability of unlimitted proliferation and maintain normal dipoid keptypes under differentiation - inhibited culture in vitro, they will differentiate into multiple lineage cell types or occur commentent and differentiation without differentiation - inhibited factors or hi presence of certain induced - factors.

    胚胎幹細胞（ es細胞） ，是存在於早期胚胎內細胞團（ icm ）的一種多潛能細胞。在體外抑制分化培養時， es細胞可無限增殖並保持正常的二倍體核型，一旦撤除分化抑制因素，或添加適當誘導劑， es細胞可發生譜系分化或定向分化。
  20. Tea cell line, derived from malignant keratinocyte, express keratin and is adopted widely in investigating the inhibitory mechanism due to its convenience for in vitro culture

    經akautoah作用后， tea細胞的增殖顯著抑制，且這種抑制呈抗體劑量依賴的方式6 『 。
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