chimeric dna 中文意思是什麼
chimeric dna
解釋
嵌合dna-
The application of dna fingerprint technique in monitoring the es cell chimeric mice
Dna指紋圖在小鼠es細胞嵌合體鑒定中的應用 -
The purposes of our work are to establish a simplified method of multiplex pcr based on chimeric primers for str loci, to develop a set of fluorescent quadriplex str system for forensic dna typing based on this method, and to validate the forensic application of the system under the guidelines of tmgdam ( the technology working group on dna analysis methods ) in order to address concerns presented in today ' s legal environment
目的本課題旨在探索一種新的str基因座復合擴增方法，我們稱為嵌合引物str復合擴增法。應用熒光標記毛細管電泳激光自動檢測技術平臺，建立一套新的法醫str基因座復合擴增體系，並按照美國dna分析方法技術工作組（ thetechnologyworkinggroupondnaanalysismethods ， twgdam ）的指導方案進行法醫學實用性研究。 -
Based on the structure and function analysis of hirudin, a potent thrombin inhibitor, and some platelet aggregation inhibitors, which contain the recognition sequence argglyasp as their functional motif, two chimeric antithrombotic molecules were designed by introducing rgd sequence to hirudin cterminus. these chimera genes were constructed by pcr and inserted into the expression vector pet21a, the constructs were confirmed by restriction enzyme digestion and dna sequence analysis. these recombinant plasmids were transformed into
經限制酶消化和dna序列分析，證明兩種重組質粒與設計完全一致。由於rgd -水蛭素嵌合基因上游連接了金黃色葡萄球菌蛋白a spa的信號肽序列，在iptg誘導下兩種嵌合分子都獲得了分泌表達，表達產物主要集中在細胞周質空間。 -
Multi - locus dna fingerprint technique was used to check the chimerism of chimeric mouse generated by injecting es cells into blastocysts and to detect whether the chimeric mouse is a germ - line chimeras. the results indicated that : the multi - locus dna fingerprint with a new synthesized probe - jl - 02, has enough polymerism and good stability, and should be very useful to monitor the chimerism in different tissues of es cell chimeric mouse and to check whether an es cell line has the capacity to enter the germ line, especially when involving strains that can not be discerned with coat color or biochemical markers
嘗試應用多位點dna指紋技術，檢測經過胚胎幹細胞es細胞途徑所獲得的嵌合體小鼠中es細胞在各種臟器中的嵌合情況檢測es細胞在嵌合體小鼠中是否實現種系傳遞。結果表明：採用新型的人工合成的寡核苷酸多聚體探針jl - 02探針的多位點dna指紋圖譜，具有足夠的多態性和很好的穩定性。 -
Construction of an expressing vector for antisense rna - ribozyme chimeric dna sequence against tomato acc synthase gene and transformation of tomato
核酶嵌合基因植物表達載體的構建及對番茄的轉化 -
Construction and identification of dna vaccine containing chimeric gene gag - gp120 of hiv
120嵌合基因核酸疫苗的構建與鑒定 -
Objectives to improve the effect of a single mtb8. 4 dna vaccine, we constructed a chimeric mtb8. 4 / hil - 12 eukaryotic plasmid by linkage of mycobacterium tuberculosis mtb8. 4 gene to human il12 gene with a simple linker ( gly4 - ser ) 3. we analyzed the immunogenicity of chimeric dna vaccine and investigated the immune responses elicited when mtb8. 4 / hil12 was presented as endogenous ag
目的：以il - 12作為分子佐劑，與結核桿菌新抗原mtb8 . 4基因連接形成嵌合分子，將其克隆到真核表達質粒中，構建成嵌合dna疫苗，研究其在小鼠體內誘導細胞免疫應答的效果及對c57bl 6n小鼠的免疫保護作用，為尋求安全、有效、廉價的結核病新疫苗打下基礎。 -
Megf and melittin was linked together by a linker into an chimeric dna, megfmel, and the chimeric dna was expressed by using pet30a as expression vector and e. coli as expression strain
值在slug砌l與54ug加l之間。在誘導表達各目的產物時誘導溫度為30c 。
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