chimeric gene 中文意思是什麼
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Construction of an expressing vector for antisense rna - ribozyme chimeric dna sequence against tomato acc synthase gene and transformation of tomato
核酶嵌合基因植物表達載體的構建及對番茄的轉化 -
To target this mitochondrial enzyme into chloroplast, the cdna sequence of mnsod was fused to a chloroplast transit peptide from a pea rubisco small subunit gene, whereas expression of the chimeric gene was driven by the camv 35s promoter
Pchlsod質粒含有煙草mnsod基因的cdna序列，與豌豆核糖體小亞基葉綠體引物肽（ tp ）的編碼基因序列構成融合基因，由35s啟動子調控。 npt基因為選擇標記基因， pgv2260為輔助質粒。 -
To get in vivo evidences that apoplast calmodulin con 1d regulate plant growth and development process, a chimeric secretion form of calmodulin binding peptide, which contains a signal peptide, a calmodulin binding domain and a c - myc epitope was constructed. the chimeric gene was introduced into arabidopsis. it was expected that the overexpression of this chimeric protein could be secreted into cell wall and bound to apoplast calmodulin, which could reduce the apoplast calmoduin concentration to make an apoplast camodulin " antisense " plant. by observing the potential phenotype change of apoplast calmodulin " antisense " plant, the in vivo function of apoplast calmodulin on plant growth and developmental process could be speculated
但這些多是採用生理學手段和藥理學方法而得出的體外（ invitro ）實驗結果，為了取得質外體cam在植物生長發育過程中發揮重要作用的invivo實驗證據，根據動物中的一些研究方法，本實驗設計並構建了帶有信號肽、 cam結合肽（ can小肽） 、 epitope （ c - myc ）融合基因的載體，並將融合基因通過真空滲入法轉入擬南芥，預期過表達的融合蛋白將會被分泌到細胞外並與質外體cam相結合，這樣就會抑制質外體cam的功能，從而可以構建質外體cam的「反義」植株，通過觀察質外體cam 「反義植株」的表型改變，就可以推斷質外體cam在植物生長發育過程中的功能。 -
The tandem expression of the human salmon chimeric ct gene in e. coli
鮭降鈣素嵌合基因在大腸桿菌中的串聯表達 -
Construction and identification of dna vaccine containing chimeric gene gag - gp120 of hiv
120嵌合基因核酸疫苗的構建與鑒定 -
Finally, the chimeric gene was inserted into binary ti vector plbj21, and cloned into agrobacterium tumefaciensgv3101 by electroporation
為了提高can小肽表達強度，本實驗構建了can小肽二連體。 -
Objectives to improve the effect of a single mtb8. 4 dna vaccine, we constructed a chimeric mtb8. 4 / hil - 12 eukaryotic plasmid by linkage of mycobacterium tuberculosis mtb8. 4 gene to human il12 gene with a simple linker ( gly4 - ser ) 3. we analyzed the immunogenicity of chimeric dna vaccine and investigated the immune responses elicited when mtb8. 4 / hil12 was presented as endogenous ag
目的：以il - 12作為分子佐劑，與結核桿菌新抗原mtb8 . 4基因連接形成嵌合分子，將其克隆到真核表達質粒中，構建成嵌合dna疫苗，研究其在小鼠體內誘導細胞免疫應答的效果及對c57bl 6n小鼠的免疫保護作用，為尋求安全、有效、廉價的結核病新疫苗打下基礎。 -
2. construction of chimeric mtb8. 4 / hil - 12 eukaryotic expression plasmid ( 1 ) construction of pci - neo - mtb8. 4 - linker ( pml ) and pci - neo - ms - linker ( pmsl ) mtb8. 4 - linker and ms - linker gene ( without stop codon ) were pcr amplified by using two oligonucleotides designed to generate nhe i and mlu i restriction sites at the 5 " and 3 " ends of the amplified fragments, respectively
3 .重組質粒在真核細胞中的表達: pm 、 pms 、 pmi和pmsl重組質粒用lipofectaminatmzo0o脂質體轉染試劑轉染cos一7細胞，進行瞬時表達， 48小時后，用rl 』 - pcr檢測目的基因在mrna水平的表達;用westemblotting檢測hil一12在蛋白質水平的表達。 -
In transgenic tobacco plants, the analysis of transient expression by monitoring 3 - glucuronidase activity revealed that a chimeric gene construct containing a 1. 2 kb pdf1. 2 promoter fused to a gus reporter gene was induced by meja
（ 1 ）從擬南芥基因組中擴增出長度約為1200bp的pdf1 . 2啟動子，與gus構建的融合基因在煙草中的表達受meja誘導。 -
To investigate the molecular basis of the stresses - induced gene regulation, the acbadh promoter - / ? - glucuronidase chimeric gene constructs containing six deletions were introduce into tobacco by agrobacterium - mediated transformation
對啟動子進行6禾缺夫構建，瞬時轉化煙草葉片表明， 6種構建都有活性。 -
They indicated that ecbp21 may have some relative with the response to stress treatments. further more, we constructed the binary vector containing ecbp21 - gfp chimeric gene and got transgenic plants by using the agrobacterium vacuum infiltration method
4 .初步開展了轉基因研究:為了進一步確定ecbp21在植物生長發育過程中的作用，構建了ecbp21植物表達二元載體，通過真空滲透法轉化了擬南芥並獲得了轉基因植株。 -
The chimeric gene was transformed into rice calli by particle bombardment. many resistance calli were obtained, and gus activity was detected. in order to confirm the causality of the mutant phenotype and osdd2 gene, we took an approach of functional knockout ofosdd2 gene, with the method rnai ( rna interference )
為了進一步驗證本基因的功能，利用獲得的包含wrky保守域的cdna片斷構建了可能產生rna干涉（ rnainterference ， rnai ）的載體，利用農桿菌轉化法轉化水稻，目前已經獲得一批轉基因苗。 -
The chimeric gene was introduced into arabidopsis through the method of vacuum infiltration which is widely used in arobidopsis tansformation. transgenic plants were screened by means of kanamycin resistance and further identified by genomic pcr and western blotting, through the phenotype observation of transgenic plants, the speculation that apoplast calmodulin may play a certain role in regulating the development and growth of plants was made
最後，融合基因被插入二元載體plbj21 ，通過電擊法轉入農桿菌gv3101 ，真空滲入法轉入了擬南芥，轉基因擬南芥通過kan抗性篩選而得到，並進一步進行了基因組pcr鑒定和western雜交的鑒定。 -
In transgenic tobacco plants, the transient - expression assay of the chimeric gene ( 4 x gcc - 35s min : : gus ) demonstrated that the 4 x gcc - 35smin promoter could respond to meja treatment and the gcc box is an important element in response to ja signaling. moreover, this experiment results would be meaningful to improve the crops characterization of resistance against various environment stresses or to study the regulation of gene expression in transgenic plants
（ 3 ）以反向的4xgcc重復序列（ placzi 4xgcc （ 》作為placzi4xgcc的突變體， 6半乳糖苦酶活性分析的結果表明，與野生型的相比，突變的gcc元件不能與jerfi 2 3 4相互作用， p半乳糖苦酶實驗不能呈現出藍色反應；證明gccbox與jerf 2 3 4是特異性結合。 -
The recombinant pcr technic was used to introduce a linking peptide klgggg to the site between scfv single chain form of the monoclonal antibody sz - 51 specific for the glycoprotein gmp140 on activated platelet membrane and uk32 low molecular weight form of pro - urokinase, to make the scfv - linker - uk32 chimeric gene. this gene was cloned into the transfer vector pbacpak9, and cotransfected with bacpak6 bsu36i digest into sf 9 cells. the fusion protein was secreted into the medium. in the fifth day after the cotransfection, the supernatant of the medium showed 107 iu ml fibrinolytic activity, higher than 25 iu ml fibrinolytic activity of scfv - uk32. elisa showed that the supernatant had the binding activity to activated platelet. wastern blotting also indicated that the supernatant could bind to the monoclonal antibody of urokinase b chain
為了提高重組導向溶栓分子scfv - uk32的溶纖活性，通過重組pcr方法在編碼scfv與uk32的堿基之間引入編碼klgggg連接肽的堿基序列，並克隆到轉移載體pbacpak9上，通過與線性病毒dna bacpak6 bsu36i digest共轉染到昆蟲細胞sf 9內，進行表達。表達產物分泌到上清中，共轉染后第5d天用纖維平板法測得sf 9細胞上清溶纖活性達到107 iu ml ，比未引入連接肽的scfv - uk32的表達活性25 iu ml高。 -
Furthermore, by inserting " anther box " element to the mutated area of two site - mutation promoters, another two promoters, ipmas and ipmal, were created. in order to study the chemical - inducible capacity of wild and modified pr - la promoters, a coding sequence of gus ( | 3 - glucuronidase ) gene was fused to their downstre am, and the chimeric genes were cloned into pbin ! 9 - based plant expression vector
為了檢測得到的啟動子驅動效率及誘導活性，將所得到的啟動子、定點突變啟動子和插入花藥盒的啟動子與gus基因連接，構建了6個植物表達載體，同時分別構建包含ipl barnase 、 ipml barnase 、 ipmal barnase嵌合基因的植物表達載體。
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