chimeric plasmid 中文意思是什麼
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To neutralize the function of cam, the cam binding domain peptide from bovine calcineurin a subunit was chosen because the pre - experiment demonstrated that bovine calcineurin could bind to calmodulin strongely in an acidic and high calcium concentration condition which is similar to the cell wall environment. in order to increase the binding ability of the chimeric secreted calmodulin binding peptide, the plasmid containing two copies of calmodulin binding domain were constructed
它共有26個氨基酸，具有信號肽的典型特徵。為了削弱質外體cam的功能，選擇了牛腦鈣調神經磷酸酶can （ calcineurin ）的a亞基的cam結合域。預實驗結果表明，在近似於胞外環境的酸性高鈣條件下can與cam有強結合。 -
Objectives to improve the effect of a single mtb8. 4 dna vaccine, we constructed a chimeric mtb8. 4 / hil - 12 eukaryotic plasmid by linkage of mycobacterium tuberculosis mtb8. 4 gene to human il12 gene with a simple linker ( gly4 - ser ) 3. we analyzed the immunogenicity of chimeric dna vaccine and investigated the immune responses elicited when mtb8. 4 / hil12 was presented as endogenous ag
目的：以il - 12作為分子佐劑，與結核桿菌新抗原mtb8 . 4基因連接形成嵌合分子，將其克隆到真核表達質粒中，構建成嵌合dna疫苗，研究其在小鼠體內誘導細胞免疫應答的效果及對c57bl 6n小鼠的免疫保護作用，為尋求安全、有效、廉價的結核病新疫苗打下基礎。 -
2. construction of chimeric mtb8. 4 / hil - 12 eukaryotic expression plasmid ( 1 ) construction of pci - neo - mtb8. 4 - linker ( pml ) and pci - neo - ms - linker ( pmsl ) mtb8. 4 - linker and ms - linker gene ( without stop codon ) were pcr amplified by using two oligonucleotides designed to generate nhe i and mlu i restriction sites at the 5 " and 3 " ends of the amplified fragments, respectively
3 .重組質粒在真核細胞中的表達: pm 、 pms 、 pmi和pmsl重組質粒用lipofectaminatmzo0o脂質體轉染試劑轉染cos一7細胞，進行瞬時表達， 48小時后，用rl 』 - pcr檢測目的基因在mrna水平的表達;用westemblotting檢測hil一12在蛋白質水平的表達。
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