chinese hamster ovary cell 中文意思是什麼
chinese hamster ovary cell
解釋
中國倉鼠卵巢細胞-
Performance of the chinese hamster ovary cell hypoxanthine guanine phosphoribosyl transferase gene mutation assay
中國倉鼠卵巢細胞次黃質鳥嘌呤轉磷酸核糖基酶基因變異鑒定的操作 -
The plasmids pci - mbl54 containing full length of mutations mbl cdna were propagated hi escherichia coli xl - 1 blue, then the extracted and purified pci - mbl54 were used to transfect dhfr ( - ) chinese - hamster ovary ( cho ) cells. after screeened with g418 and cloned, 4 g418 - resistent clones were randomly selected for detection of mrna expression by rt - pcr and molecular beacons. it was found that all of the 4 positive cell clones expresse mbl analogue as detected in transcription level
抽提、鑒定、純化重組質粒后,脂質體轉染法將重組質粒導入中國倉鼠卵巢細胞( cho - dhfr ~ - )中, g418選擇轉染子並克隆化培養,經rt - pcr和分子燈塔探針雜交鑒定其mrna轉錄,獲得4株穩定表達54位密碼突變型mbl的cho細胞。 -
Chinese hamster ovary cell, cho cell
目的研究中國倉鼠卵巢上皮細胞
分享友人