cholesterol oxidase 中文意思是什麼

cholesterol oxidase 解釋
膽固醇氧化酶
  • cholesterol : n. 【生物化學】膽固醇，膽甾醇，異辛甾烯醇。
  • oxidase : n. 氧化酶。adj. -dasic
  1. As a excellent material for preparation of biomembrane, cellulose acetate ( ca ) is a fine carrier for the immobilization of cholesterol oxidase ( cod )

    醋酸纖維素（ ca ）作為一種優異的生物傳感用薄膜制備材料，是膽固醇氧化酶（ cod ）優良的固體載體。
  2. Cholesterol oxidase is a type of protein that was found to have pest - resistant activity only years ago, its toxity to such sensitive pests as cotton boll weevil is euqivalent to previously found bt. endotoxins. the protein can also inhibit the growth and development of cotton boll worm

    膽固醇氧化酶對其敏感害蟲?鞘翅目的棉鈴象甲的毒殺能力與早期發現的bt .毒蛋白相當，對鱗翅目的一些害蟲如煙蚜夜蛾等也有較強的抑制其生長發育的作用。
  3. Moreover, together with cholesterol esterase and catalyse, cholesterol oxidase can be used as test reagent to determine the total cholesterol content in blood plasma for the diagnosis of such as cardiovascular reflex and atherosclerosis clinically

    另外，在臨床上膽固醇氧化酶可以與膽固醇脂酶和過氧化氫酶一起作為血漿總膽固醇含量的檢測試劑，用以診斷冠心病、動脈粥樣硬化等心腦血管疾病。因此，對膽固醇氧化酶的研究越來越受到重視。
  4. The nucleotide ( nt ) sequence of the insert in phz1754 is 2299bps in size. computer assisted analysis of the sequence revealed an open reading frame ( orf ) with a g + c content of 70. 3 % that would encode a protein of 552 amino acids ( aa ). the nt seque nce comparision revealed that the orf in the sequenced region exhibits 85 % dna sequence homology with the cholesterol oxidase gene choa of streptomyces sp

    對phz1754進行外切核酸酶（ exonuclease ， exo ）順序缺失，獲得單向長度漸減重疊的系列突變體，核苷酸序列測定顯示出該ecor - sal片段的精確大小為2299bps ， frameplot程序分析揭示出該區域一個完整的開放閱讀框（ orf ）的存在，其大小為1656bps ， g + c含量為70 . 3 ，編碼552個氨基酸，利用blastsearch程序將orf的核苷酸序列及推導的氨基酸序列與因特網上基因及蛋白質數據庫進行綜合比較，發現無論在核苷酸水平還是在蛋白水平上，該orf均與膽固醇氧化酶表現出同源性，而且與鏈黴菌膽固醇氧化酶同源性最高，說明該orf編碼膽固醇氧化酶基因。
  5. Because of its high toxity activity and complementary inseticidal spectrum with bt. endotoxin which is now being used universally, the cholesterol oxidase is acknowleged as one of the representitive of the insecticidal protein of the second generation

    因為它的毒殺能力強，而且其殺蟲譜又與現在廣泛使用的bt .毒蛋白具有一定的互補性，所以被認為是繼bt .毒蛋白之後的第二代殺蟲蛋白的代表之一。
  6. As heterologous probe and subsequently show to code for desired enzymatic activity. after a serial of subcloning coupled with southern hybridization and enzymatic activity assay, the functional s. griseus atcc14811 cholesterol oxidase gene ( chog ) was localized onto 2. 3kb ecori - sall fragment

    對phz1140和phz1141進行bamh及bgl的酶譜分析及與choa探針的雜交，將膽同醇氧化酶基因初步定位在8 . 8kbbamh和9 . 9kbbgl片段上。
  7. Therefore, research on cholesterol oxidase has become more and more important. a 1. 6 kb dna fragment was amplified using pcr method from genomic dna of rhodococcus equi. 4 - 2g2

    本研究採用pcr方法從分泌膽固醇氧化酶的馬紅球菌菌株4 ? 2g2基因組dna中，擴增出特異dna片段，該擴增片段全長約1 . 6kb 。
  8. In this study, a new gene c / wew, encoding cholesterol oxidase, was isolated from rhodococcus equi. 4 - 2g2 found in china, which may be useful in clinical diagnosis healthy food and pest management in agriculture. in addition, the gene has been expressed successfully, the expression product has cholesterol oxidase activity, thus this work provided theoretical basis to the development of genetic engineering of cholesterol oxidase

    本研究從我國自行分離的馬紅球菌4 ? 2g2菌株中分離到一種編碼膽固醇氧化酶的新基因choew ，它將可應用於臨床檢測、保健食品和農業抗蟲等領域；同時利用原核表達載體成功的在大腸桿菌中表達了目的基因，表現出膽固醇氧化酶酶活，為膽固醇氧化酶的基因工程利用開發作了一定的理論實驗基礎研究。
  9. The enzyme retained full activity after being treated at room temperature for 1 hour at ph between 4. 0 and 11. 5. the enzyme can be incubated at 50 for 4h with only less 50 percent loss of activity and is stable in the frozen state. when streptomyces griseus atcc14811 was cultured in 10. 3 % sucrose yeme liquid medium, production of extracellular cholesterol oxidase increased for 5 days before decrease

    利用硫酸銨鹽析及deae -纖維素離子交換柱層析提取純化灰色鏈黴菌atcc14811發酵上清液中的膽固醇氧化酶，理化性質研究表明酶作用晟適ph為8 . 0 ，最適溫度為45 ， ph穩定范圍在ph4 . 0 - 11 . 5之間，在50條件下保溫4h ，仍保留54酶活力。
  10. Air of this advanced development provides efficient methods for protein engineering. in this study, a new two - step procedure has been developed for the detection of cholesterol oxidase activity in streptomyces strains. streptomyces griseus atcc14811 was confirmed to produce cholesterol oxidase and it also showed homologies with the cholesterol oxidase gene ( choa ) from streptomyces sp

    本實驗設計了簡單易行的鏈黴菌膽固醇氧化酶活性測定指示平板，結合用鏈黴菌sa - coo （ streptomycessp . strainsa - coo ）膽固醇氧化酶基因（ choa ）為異源探針的southern雜交，確證了灰色鏈黴菌atcc14811 （ streptomycesgriseusatcc14811 ）具備產生膽固醇氧化酶的能力。
  11. The precursor of cholesterol oxidase of atcc14811 was predicted with a calculated mr of 60020. the cholesterol oxidase produced by streptomyces griseus atcc14811 was purified from the culture broth by procedures including steps for preparation of crude enzyme solution, ammonium sulfate fraction and column chromatography on deae - cellulose

    Chog與chob （ brevibacteriumsterolicum ）的氨基酸同一性和相似性分別為53和70 ，根據推導的氨基酸序列chog蛋白質前體的分子量為60020 ， chogn端區域顯示信號肽特徵。
  12. The expression mixture were eletrophoresized by sds - page, revealing a specific 56 kd protein in bl21 expression system, which is of the same size in expectation. a colorimetric assay for measuring cholesterol oxidase showed the cholesterol oxidase activity of the expression product

    其結果經sds ? page分析表明：以pet - his為表達載體，大腸桿菌bl21 （ de3 ） plyss為宿主菌的重組表達系統表達出約56kd的特異蛋白，蛋白大小與預期結果相一致。
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