chromatography affinity 中文意思是什麼

chromatography affinity 解釋
親合色譜法
  • chromatography : n. 【化學】層析，色層（分離）法。
  • affinity : n 1 姻親關系 〈cf consanguinity〉; 密切關系。2 （語言等的）類似，近似。3 （男女之間的）吸引力，吸...
  1. Studies of the chitosan microspheres prepared as affinity chromatography carrier and used for purifying thrombin from bovine blood plasma

    殼聚糖微珠作為親和層析載體的制備及其用於分離牛凝血酶的研究
  2. 6 - phosphogluconate dehydrogenase ( 6 - pgadase, ec 1. 1. 1. 44 ) was isolated by homogenate, ammunium sulfate fractionation, deae - sepharose chromatography, blue - sepharose affinity chromatography and gel filtration with sephadex g - 200 from bacillus subtilis, and some properties of the enzyme had been studied. a 113. 8 - fold purification was obtained with a 8. 2 % yield. the purified enzyme moved as a single electrophoretic band in page

    將枯草芽孢桿菌超聲波破壁后的粗提取物進行分段鹽析、 deae - sepharose陰離子交換柱層析， blue - sepharosecl - 6b特異結合柱層析和sephadexg - 200凝膠過濾等純化步驟，得到聚丙烯酰胺凝膠電泳為單一蛋白區帶，比活為1 . 46u mg的酶制劑。
  3. Purified fusion protein gst - hnadc3 was used as an immunogen to inoculate rabbits and the antibody against the gst - hnadc3 fusion protein was raised, and was purified by gst sepharose 4b affinity chromatography to remove the antibody against gst

    Ptg誘導表達，超聲破碎細胞后，採用親和層析方法純化融合蛋白gst十nadc3 ，並以此為抗原免疫紐西蘭株白兔制備融合蛋白抗體。應用親和層析的方法對gst十nadc3融合蛋白抗體進行純化，以去除抗gst抗體。
  4. The xerocomus spadiceus lectin, xsl, was isolated from extracts of fruiting bodies of the mushroom xerocomus spadiceus using a procedure that involved ( nh4 ) 2so4 precipitation, anion exchange chromatography on deae - cellulose, affinity chromatography on affi - gel blue gel, cation exchange chromatography on cm - cellulose, and gel filtration by fast protein liquid chromatography on superdex 75

    從磚紅絨蓋牛肝菌（ xerocomusspadiceus ）子實體粗提物中，經過deae -纖維素陰離子交換層析、 affi - gelbluegel親和層析、 cm -纖維素陽離子交換層析和superdex75fplc凝膠過濾，純化了磚紅絨蓋牛肝菌凝集素。
  5. Conentional methods such as fractionation of complex clinical samples by ionic exchange chromatography and new methods such as enriching low abundant proteins by affinity capture with a combinatorial library of ligands14 proide much needed tools for processing complex biological and clinical samples for proteomics research

    傳統（如對復雜臨床樣品通過離子交換層析進行分離）和現代的方法（如通過親和捕獲與配體庫的組合富集低豐度蛋白）為處理用於蛋白質組學研究的復雜的生物學與臨床樣品提供了極需的工具。
  6. Gfral was high expressed in e. coli after iptg induction. by ni2 + chelation affinity chromatography, the purified recombinant gfral protein were obtained. based on the evolutionary trace method, multiple sequence alignment and dendrogram construction were then carried out by the clustalx program and constructed a phylogenetic tree from a multiple sequence alignment for a protein family

    2 . gdnf的表達及其對pc12基因工程細胞的影響用本教研室已構建好的表達質粒pet一gdnf轉化大腸桿菌bl21 （ de3 ） ，經lmmipto誘導gdnf表達，並在niz氣nta柱上進行純化，稀釋復性后，純度達90 %以上。
  7. In order to detect the effect of human sperm mannose - ligand receptor on the fertilization ability, in the study reported here mannose - ligand receptors ( mrs ) were purified from human sperm by modified mannose - agarose gel affinity chromatography coloumn and determined protien concentration by lowry, preincubated zona - free hamster oocytes with four purified mannose - ligand receptor ( pmr ) concentrations before sperm penetration assay ( spa ) to test the pmrs cell biology nature of inhibition to fertilization

    本研究用改良后的親和層析法分離純化mr ， lowry法測定其蛋白質濃度，在精子穿透試驗（ spermpenetrationassay ， spa ）模型中定量研究其對精卵融合能力的影響並檢測其細胞生物學活性；以已知濃度的pmr （ purifiedmannose - ligandreceptor ）干預精子半透明帶試驗，觀察用pmr預處理半透明帶對精子與透明帶結合的影響。
  8. A novel chitosan ( cts ) - binding protein was isolated from the non - heading chinese cabbage leaves by chitosan affinity chromatography and partially characterized

    說明cts在調控氨同化關健酶gdh和gs活性時，可能有ca 』 +信號系統的參與。
  9. Fusion expression of m - centrin in e. coil bl21 was performed by induction of fptg. fusion protein was digested by ppase and was purified by gst chelating affinity chromatography and ion exchange chromatography. the final products were checked by sds - page gel

    融合蛋白gst - m - centrin菌體經過超聲波裂解后得到的上清夜經過gst親和層析後用prescissionprotease （ ppase ）酶切，酶切產物再次經過gst親和層析和hitrapq陰離子交換層析兩步柱層析純化后，得到純度較高的的m - centrin 。
  10. We purified the fusion mpt64 protein by the nickel - ion affinity chromatography

    通過鎳離子親和層析獲得相對純的mpt64重組蛋白。
  11. Gst - eorabl fusion protein and truncated gst - deeorabl fusion protein were purified by gst affinity chromatography and analyzed by cleaving with thrombin protease

    全長型融合蛋白經凝血酶酶切和進一步親和純化得到達電泳純的游仆蟲rab蛋白（ eorab1 ） 。
  12. Crotalaria mucronata lectin ( cml ) was purified from seeds of crotalaria mucronata by extraction, fraction with ( nhi ) 2864, hog gastric mucin - sepharose 4b affinity chromatography and followed by gel filtration of sephacryl s - 200 hr. cml agglutinated type a human red blood cells specially. the purified cml gave one band pel e ' ectronhoresis and on sds nolvacrvlamide gel electrophoresis

    野花生豆（ crotalariamucronata ）經磨粉、浸取、硫酸銨分級沉澱、豬胃粘蛋白- sepharose4b親和層析、 sephacryls - 200hr分子篩層析可得到一表觀分子量為103kd且對a型血紅細胞專一凝集的野花生豆凝集素（ crotalariamucronatalectin ， cml ） 。
  13. In order to attain bioactive glycoprotein, glycoprotein of the leaves of camellia sinensis, was purified from coarse glycoproteins purified by sephadex g - 100 gel chromatography, by cona - sepharose 4b affinity chromatography

    為了純化天然糖蛋白，進行了茶樹葉糖蛋白的cona - sepharose4b親和層析，從sephadexg - 100凝膠過濾收集的粗糖蛋白中，分離茶樹葉糖蛋白。
  14. Sepharose affinity chromatography. the apparent molecular weight of purified ghrp - scu - pa - 32k was 33kd by sds - page and mass spectrometry and its specific activity was 150000iu mg protein, according to fibrin plate determination

    經鋅離子螯合親和柱純化的產物， sds - page顯示為單一蛋白條帶，分子量為33kd ，質譜法測定分子量也為33kd 。
  15. The total entropy production for affinity chromatography and the equations of mass and heat fluxes under the condition of the multi - field synergy were obtained

    得到了親和層析過程的總熵變表達式，多場協同條件下質量通量和熱量通量的唯象表達式以及相應的傳質和傳熱系數。
  16. Fusion protein gst - hdt was purified by gstrap affinity chromatography

    將工程菌株發酵所得酶液，經gstrap親和層析，得到融合蛋白gst - hdt純品。
  17. One is a combination of ion exchange chromatography on q sepharose ff, hydrophobic interaction chromatography on phenyl hp, gel filtration chromatography on sephadex 200 and higher resolution ion exchange chromatography on mono q. the other is a combination of ion exchange chromatography on q sepharose ff, two affinity chromatography on con a sepharose 4b and benzamidine sepharose 6bcl sequentially

    上清液中重組蝗蛇毒類激血酶的純化是通過設計的不同分離純化方法組合進行的，並對每一種組合進行了活力與純度及口收率的評價，結果表明兩種組合方式都具有一定的實用性和可操作性。
  18. A method for preparation of antithrombin ( at - ) concentrated from human plasma ' s fraction has been described. after preliminary treatment of plasma with the cold ethanol, the isolation of at - iii from the precipitate was performed by affinity chromatography on heparin - sepharose cl - 6b ; desalted by dialysis and concentrated

    從人體血漿中提取較高純度的抗凝血酶（ at - ）的，通過低溫乙醇處理血漿，得到的沉澱經肝素-瓊脂糖兩次親和層析，並透析、濃縮獲得at -的初品。
  19. Then the product was purified by glutathione sepharose 4b chelation affinity chromatography, and upper purified gst - gnrh / trs fusion proteins was received for further the foundations established in the scientific research and the real application

    表達產物經谷胱甘肽瓊脂糖4b親和層析純化后，得到了純度較高的gst - gnrh trs融合蛋白。為進一步科研和實際應用奠定基礎。
  20. Purification and renaturation of recombinant human cu, zn - sod by metal - chelating affinity chromatography

    金屬螯合親和層析純化和復性重組人銅鋅超氧化物歧化酶
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