clone method 中文意思是什麼

clone method 解釋
克隆方式；復制方法
  • clone : n. 1. 【生物學】純種細胞，無性系。2. 復製品；（幾乎）一模一樣的人。3. （不動腦筋）機械行事的人；機器人。vt. 把…培養為純種細胞；無性繁殖；〈比喻〉復制。
  • method : n 1 方法，方式；順序。2 （思想、言談上的）條理，規律，秩序。3 【生物學】分類法。4 〈M 〉【戲劇】...
  1. Following results were achieved from this thesis. ( 1 ) a rapid and easy pcr method was established to clone type ii pha biosynthesis genes

    論文取得了如下研究結果： （ 1 ）建立了一種快速克隆型pha合酶基因的方法。
  2. 2. an up - dated method was employed to purify tumv in this research. using the protease k method, we acquired the viral genome - rna. a pair of specific primers was designed and synthesized based on the nucleotide sequences of tumv coat protein genes reported before and rt - pcr was used to clone the cp genes of the six tumv isolates

    應用改進的蕪菁花葉病毒的提取方法從病葉中提取病毒粒體，應用蛋白酶k法從病毒粒體中提取病毒rna基因組，根據已報道的tumv的cp基因序列，設計併合成了一對特異引物，利用rt - pcr法克隆了6個分離物的外殼蛋白基因，與克隆載體puc19連接后通過熱激法轉化大腸桿菌dh5 。
  3. There was no difference in other biologic characteristic of mscs between the two separation method, such as cell anchorage ratio and clone formation ratio. ( 2 ) plga film presented uniformity frame with no protuberance and fissure under scanning electron microscopy ( sem ). big aperture with smooth wall and average 400 m i n size running - through each other was observed in porous plga substrate, around the big aperture there were many round micropores about 5 m size. all of the structure were equal and uniform, which satisfied the further research work. ( 3 ) mscs adhesion at earlier time was promoted by biotiegenrafter 3h, cell number was ( 1. 5 0. 18 ) 105 in the plga film coated with biotiegen group, which was significantly higher than that in plga film group ( p < 0. 01 ) and higher than that in coverslip group ( p < 0. 05 ), which cell number was ( 1. 04 0. 21 ) 105. after 6h and 12h biotiegen could not promote cell adhesion, and cell proliferation and alkaline phosphatase ( alp ) activity were not promoted dramatically during 9 days. ( 4 ) cell adhesion was promoted by fibronectin or collagen type i

    G ） i型膠原、纖維粘連蛋白促進細胞增殖，細胞接種后3 、 6 、 gd三個檢測時間點，實驗組細胞均明顯高於對照組。與1型膠原相比，纖維粘連蛋白刺激作用更強。 ） i型膠原、纖維粘連蛋白尚能誘導mscs細胞向成骨細胞分化，不僅表達成骨細胞標志物ocn 、 alp 、 opnmrna ，而且堿性磷酸酶活性明顯增高，堿性磷酸酶及鈣結節7第四軍醫大學博士學位論文一染色均強陽性， i型膠原組mscs細胞堿性磷酸酶活性較fn組更高，有顯著性差異；同時，兔疫組化染色表明，經纖維粘連蛋白作用的mscs1型膠原表達陽性。
  4. It has been reported that the eiav s2 is not essential and does not appear to affect virus infection and replication in vitro. thus, we introduced a his - tag into the s2 gene of an eiav infectious molecular clone recombinant plasmid ( pok8266 ) by using soe pcr method and obtained a new recombinant plasmid with his - tag, designated as eiav - pok8. 2 - his

    本研究應用已構建好的eiav驢白細胞弱毒疫苗株的感染性分子克隆載體（ pok8266 ）為模板，通過soepcr方法在感染性分子克隆載體的s2基因獨特區內引入突變點，形成含有酶切位點（ nspv ）的突變體（ p1p4 ） 。
  5. The objectives of this study are to clone the ban fragment of brassica napus and to apply the floral - dip in the transformation of oilseed rape to establish a convenient method for oilseed rape breeding. ban is dfr - like gene in chalcone synthesis pathway functioned as a negative regulator, and has been well studied in arabidopsis thaliana

    為利用轉基因技術創造甘藍型黃籽油菜，本文克隆了與甘藍型油菜（ brassicanapus ）色素生物合成有關的ban基因同源片段，並探索了甘藍型油菜花序浸泡法轉基因新方法。
  6. Studies of genes related to heart development in drosophila contribute to reveal the mechanisms of human heart development and the congenital heart diseases. to clone and identify new genes that control the heart development, by a way of chemical mutagen, ems, we have established 1, 200 balanced - lethal lines on chromosome 2 and 3. with the screening the 330 stocks with immunochemical method using heart - specific antibody, mab. no. 3, we detected 60 lethal lines showing heart mutant phynotype

    為了克隆和鑒定控制心臟發育的新基因，本研究利用化學誘變劑甲磺酸乙酯大規模地誘變果蠅，並且建立了1200個第二和第三染色體的平衡致死系，利用心臟組織特異抗體mab . no . 3對其中330個品系進行免疫化學方法篩選，觀察到有60個致死系表現出心臟突變表型， 20個品系的心臟突變表型有待進一步證實。
  7. The total rna was purified from the germ in the liquid by the guanidine isothiocyantehod method, then the total rna digested by dnase that had not rnase was used for rt - pcr. i change the magnesium ion dencity in the pcr system in order to optimize the pcr condition. at the end i selected the magnesium ion density as 1. 25 mm. the production of rt - pcr was inserted directionally into pgem ? z ( ampr ). the pgem ? z ( ampr ) was used to transform e coli jm109. i got a positive clone through culling and identificatin. the dna sequence inserted into pgem ? z ( ampr ) was sequenced and blasted with the cdna sequence of the # - mannanase mature peptide that got from genbank

    分取誘導培養液中的菌體，用異硫氰酸胍法提取總rna ，總rna再經無rna酶的dna酶處理後用于rt ? pcr 。在pcr擴增目的基因時，通過優選擴增體系，使鎂離子濃度為1 . 25mm時rt ? pcr可順利地獲得目的基因，並能定向克隆到載體pgem ? 3z （ amp ~ r ）中。用克隆載體轉化宿主大腸桿菌jm109 ，通過篩選獲取陽性克隆子，對陽性克隆子進行酶切與pcr鑒定，並對載體中插入的目的基因進行測序。
  8. Gene cloning differential fragment gha27 was produced by cdna - aflp method employed to compare the gene expression in developing anthers between the male sterile and fertile plants of cotton dong a. a cdna clone, designated gharf ( gossypium hirsutum adp - ribosy lation factor ), was isolated from a cotton ( gossypium hirsutum l. ) cdna library using gha27 as probe. 2

    棉花arf基因的克隆選取棉花洞a雄性不育株和可育株花藥cdna - aflp差別顯示的差異片段gha27 ，用地高辛標記作為探針，篩選棉花洞a花藥的cdna文庫，克隆得到了棉花arf基因的cdna全長序列，將其命名為gharf （ gossypiumhirsutumadp - ribosylationfactor ） 。
  9. Created or discovered and developed, plant varieties of any botanical genus and species, including clone, line, hybrid, and rootstock, irrespective of the method artificial or natural of their production, hereinafter referred to as arieties. ? p

    已被創造或發現及開發的任何植物屬別或種別，包括無性繁殖系clone品系line雜交種與根莖的植物品種，不論其生產方法天然或人工以下稱為植物品種。
  10. Objective : to clone and sequence the cdna encoding metalloproteinase from the venom of agkistrodon acutus from northen mountain area of guangxi province. methods : one step method was used to extract total rna from the venom of agkistrodon acutus found in northern mountain area of guangxi province. different kinds of cdna encoding metalloproteinase were amplified by one step method ( rt - pcr and pcr reactions occurred in the same tube ) using different primers

    方法：從桂北五步蛇毒腺中抽提總rna ，利用不同的引物，採用一步法（ rt - pcr和pcr在同一管內進行）擴增出不同的dna條帶，利用平端連接的方法將pcr擴增產物克隆至pgem - teasy載體，轉化大腸桿菌jm109 ，挑選白色菌落提取質粒，用pcr對其進行鑒定，直接利用純化pcr產物或提取陽性菌落質粒進行測序。
  11. One positive clone was obtained from the libraries with casein flat and electrophoresis comparison method. analyzed the sequencing result, the positive clone include the open read frame of mlap gene and upper regulatory sequence

    序列測定分析表明，此重組質粒包含有長度為1377bp低溫堿性蛋白酶基因的完整的開放讀碼框架（ orf ）和上游基因調控序列。
  12. Osdd2 gene existed as a single copy gene in the rice genome based on the result of southern blot analysis. we took a pcr method to clone the cdna of osdd2 gene

    使用克隆的邊端插入序列為探針對水稻（中花11品種）進行southern雜交分析，表明osdd2基因在水稻基因組中是以單拷貝存在。
  13. To deal with the two - class classification problem, a new strategy of integrating the genetic algorithm and the lvq artificial networks is adopted to reduce the dimensions in high dimension space. using this method, the classification accuracy is 100 % to leukemia dataset, and 91. 27 % to clone cancer dataset

    把遺傳演算法和lvq神經網路結合進行高維空間的特徵選擇，以解決兩類別的樣本分類問題，並利用白血病和大腸癌基因晶元數據進行了實例計算，分別達到了100和91 . 27的準確度。
  14. Major difference of hn proteins lies in no. 18 - no. 75 amino acid residues on n - terminal which includes three active sites of hn protein. the influence on biological action, caused by the difference of hn protein, is needed to study further. hn gene has only four glycosylation sites, which is 1 or 2 less on amount than that of other strains. so, this difference can be take on as a natural mark of ndv b95 strain furthermore, the fragment encoding hn gene was excised from the positive clone pgem - hn with sail and saci enzyme, and purified by agrose gel fraction method. then the fragment was subcloned into the pet - 28c expressing vector digested by sail and saci restriction enzyme

    作者將正確的重組克隆質粒pgem - hn用saii 、 saci雙酶切，電泳回收目的片段，將其亞克隆到經saii 、 saci雙酶切處理的pet - 28c中，轉化大腸桿菌tgi感受態細胞，得到的轉化子經pcr鑒定和酶切分析，篩選出符合閱讀框的重組子，構建成重組表達質粒pet - hn ，並在大腸桿菌bl _ （ 21 ） （ de _ 3 ）宿主菌中成功地表達了含目的蛋白的融合蛋白，融合蛋白的分子量約66kd ，加入iptg誘導8h后，蛋白表達幾乎達到最高水平。
  15. This method returns the clone node

    這個方法返回克隆的節點。
  16. Clone principle is led into evolutionary computing, and a hybrid algorithm is combining antibody clone strategy with fuzzy c - means clustering method is given. it is used in intrusion detection

    提出將人工免疫與模糊c -均值聚類技術相結合進行聚類，從而實現對異常行為的檢測的演算法。
  17. This method should return a clone of the metadata

    此方法應返回元數據的復本。
  18. Main works and their important results are showed as following. firstly, the cdna encoding bullfrog gh is amplified by use of the total rna, extracted from adult bullfrog ' s pituitary, as the template, pi ( 5 ' - cggatcc atg gct tca ggg tta ggc ) and p2 ( 5 ' - cgaattc tta aaa ggt gca gtt gct ) as the primer pair and one particular cdna product, 660bp, was obtained after the rt - pcr - the product was purified by using dna agarose gel extraction method. after the purified cdna and pmd18 - t vector were ligated at 16 over night, the ligation products were transformed into e. coli strain dh5a and then the transformants were screened by clone pcr method

    首先，以成體牛蛙的腦垂體總rna為模板，進行rt ? pcr擴增得到約660bp的特異性pcr產物帶，切下瓊脂糖凝膠中的特異產物帶進行cdna膠回收，將cdna與pmd18 ? t載體進行t ? a克隆連接並轉化到dh5 e . coli后，進行菌落pcr 、質粒酶切鑒定，篩選出陽性菌株，測序結果經blast分析，與已報道的牛蛙生長激素基因高度同源，證實此陽性克隆為牛蛙生長激素基因轉化子，命名為pbfgh 。
  19. After 3 rounds of screening, 10 of 20 clones were identified as positive clones and all the 10 phage clones sharing the same amino acid sequence : ehmaltypfrpp, and these positive 12mer phage clone peptide can bind with tnfa specifically. the results suggests that this method is very specific and simple for screening of binding epitope to small peptide molecule from phage display peptide library

    在以噬菌體環七肽tnfa模擬肽克隆刁k naqsoc ）為靶對噬菌體隨機十二肽庫進行h輪篩選后，挑取20個克隆，經elisa鑒定有10個克隆均與t 』 nfa結合，測序結果顯示它們均為同一氨基酸序列： ehmaltypfrpp 。
  20. The main contents and results were as follows : 1. the establishment of sd rat fibroblast for the donor of nuclear transfer the method of single cell cloning by micro - manipulating was improved to purify fibroblast. as a result, steady fibroblast can be obtained and the rate of the clone formation is 92. 71 %

    主要內容和結果如下： （一） sd大鼠成纖維細胞系的建立將單細胞顯微法改進為單細胞集落克隆純化，能快速、有效建立細胞系，並可獲得92 . 71克隆率。
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