cloned 中文意思是什麼

cloned 解釋
克隆陰謀
  1. It was originally cloned at the dna level from the unicellular green alga chlamydomonas reinhardtii

    最初，它是在一種綠藻chlamydomonasreinhardtii中發現的。
  2. The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss

    將缺失型pap基因克隆到植物表達載體pbi121中，通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中，然後採用葉盤法，在該農桿菌的介導下將pap基因導入普通煙草中，經過卡那黴素抗性篩選，最後獲得了轉pap基因的工程煙草植株，摩擦接種煙草花葉病毒（ tmv ） ，與非轉基因煙草相比，能夠推遲癥狀表現達2月之久，說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。
  3. The chs gene which is obvious correlated with anthocyanin accumulation was cloned as well

    對與花青素積累有明顯相關的chs基因進行了克隆。
  4. It has been established the existence of a few pathways controlling flowering of plants in genetic research of flowering of arabidopsis thaliana and antirrhinum majus. many genes related to flowering have been identified and cloned, but the regulation mechanism of gene ' s expression is still unclear

    通過模式植物擬南芥和金魚草開花機理的研究，現在已經明確植物的開花途徑有多條，並識別和分離了許多與開花有關的基因，但這些基因如何在植物生長發育過程中進行特定時空的表達及其調控一直是科學家研究的一個黑匣子。
  5. A rabbit was infected with a cloned yntatl, blood was collecting from from the rabbit every 3 days after infection within 30 days, 10 clonal trypanosome populations were gotten, infecting a new rabbit by the last non - cloned trypanosome population. repeated above all, thus infected 5 rabbits sequentially. twenty different vats ( variant antigen type ) were monitored and characterized from those fifty mono - clonal populations by indirect immunofluorescence test ( ift ) and avidin biotin enzyme immunoassay ( abc - eia )

    用伊氏錐蟲雲南水牛單克隆株yntat1感染兔，感染后30天內，每3天從兔血中分離錐蟲並單蟲克隆，最後一個未單蟲克隆的蟲株感染另一隻兔，重復以上操作，這樣順序感染5隻兔子，共獲得50個單克隆錐蟲種群（ tp ） ，經間接免疫熒光和abc酶標試驗鑒定共為20個抗原性互不相同的抗原變異體（ vats ） 。
  6. In our experiment, the specific fragment was amplified from transgenic bobwhite genome dna at annealing temperature 61 by using high - fidelity pfu dna polymerase and cloned into clone vector pgem - 7fz ( + ), then sequenced. the cloned sequence was completely identical to the sequence which was issued in genbank

    本實驗採用了高保真pfudna聚合酶，在退火溫度61條件下從轉基因bobwhite品種基因組dna中擴增出特異性片段，將此片段插入克隆載體pgem - 7fz （ + ） ，經測序和序列分析表明，所擴增得到的片段含有bar基因完整的讀碼框，並且序列與genbank中發表的序列完全一致。
  7. In the experiment, the full code sequence of bar gene was cloned by pcr from transgenic herbicide resistant bobwhite wheat and checked. it was expressed in e. coli and its protein was determined. after having been properly modified, the bar gene which correctly codes pat was cloned into binary vector pbi121 and then transferred into lba4404 by triparantal crossing, which is the prerequisite work for genetic transformation

    本實驗從抗除草劑轉基因bobwhite小麥中，利用pcr克隆的方法擴增出bar基因全長，並在原核表達系統中表達，鑒定表達蛋白的活性，將能夠正確編碼ppt乙酰轉移酶的bar基因片段，經過適當的修飾構建入真核表達載體。
  8. Not only did the cloned siblings show distinct food preferences and temperaments, but they also varied in physical characteristics : some had more bristly coats or fewer teeth than others did

    這些以復制方式產生的手足，不但各有獨特的食物偏好及個性，身體特徵也各不相同，有的皮膚長滿了硬毛，有的則是牙齒數目比其他的少。
  9. Degenerate oligonucleotides to highly conserved regions of cucumis melo 1 - aminocyclopropane - 1 - carboxylic acid ( acc ) oxidase gene were used to prime the amplification of fragment of 128bp by ploymerase chain reaction ( pcr ) in samples of genomic dna from fruit of cucumis melo l. cv hetao flesh, which was cloned into plasmid vector pmd - 18 - t. the clon of antisense orientation were selected, and it was inserted downstream of camv35s promoter and enhancer " " of tmv into the plant expression vector pbinyxw, antisence expression vector pbinya was constructed. at the base that pollination and fertilization of cucumis melo l. cv hetao was studied, using pollen tube pathway transformate cucumis melo l. cv hetao, 76 fruit had been obtained, moreover, hardness and content of sugar were analysed

    本實驗以河套蜜瓜果肉基因組dna為模板，用甜瓜acc氧化酶基因特異寡核苷酸鏈為引物進行pcr擴增，得到128bp的擴增產物。將得到的擴增產物克隆到質粒載體pmd - 18 - t上，篩選反向克隆，然後將其反向構建到植物表達載體pbinyxw的camv35s啟動子和tmv增強子「 」的下游，構建成反義表達載體pbinya 。並在對河套蜜瓜授粉受精生物學研究的基礎上，通過花粉管通道法轉化河套蜜瓜，共獲76顆瓜，並進行了硬度和含糖量的分析。
  10. Even so, on experimenter can predict which of the remaining fragments will be cloned.

    即使如此，任何實驗工作者仍不能預料剩下的片段中哪個將無性繁殖。
  11. Methods and applications of inserting a stop codon into a cloned gene with pcr

    在已克隆基因末端添加終止密碼的方法與應用
  12. The contents of this studies include : 1 ) according to the researches on the correlation between the function and structure of the cmiv from bombyx - moxi before by others, especially by lixinlal in naigin normal university of china, we have designed and sythesized the mutation i of the gene of cmiv that was different from the natural cmiv about 50 % in amino sequence, using the favorable condon of the ecoli. after cheked the result of synthesis by sequence, we have cloned the gene into 3 " of the gene of thioredoxin in the thio - fusion expression vector ( ptxfus ), and the fusion protein of thio - cmiv was highly expressed in soluble form

    本研究的內容包括：一、在前人對抗菌肽cmiv研究的基礎上，對n端和c端進行氨基酸保守變換，設計和合成了該基因，充分使用大腸桿菌偏愛的密碼子，並將該基因5端與硫氧還蛋白基因3端融合，通過ptxfus表達載體獲得較高可溶性表達（在15 sds - page膠上可見明顯的表達蛋白帶） 。
  13. Two primers, designed according to the conserved regions of ban gene in arabidopsis thaliana, were used to amplify the ban homologous fragments from the genomic dna of brassica napus, b. chinensisl, b. juncea, a. thaliana and other cultural plants of cruciferae. the very similar pcr fragments were obtained from all the amplifications, which implicated that ban may be a conserved gene existing widely in the genomes of cruciferae. pcr fragments were cloned and confirmed by restriction enzyme digestion

    參照擬南芥（ arabidopsisthaliana ） ban基因與cdna設計引物，對甘藍型、白菜型、芥菜型油菜，擬南芥及其它十字花科栽培品種的基因組dna進行pcr擴增，均擴增出與擬南芥ban基因擴增片段大小極其相似的dna片段，提示ban可能廣泛存在於十字花科植物中。
  14. Many organisms produce enzymes, termed photolyases, that specifically bind to these damage products and split the via a uv - a / blue light - dependent mechanism ( photoreactivation ), thereby reversing the damage. these two photolyase are specific for either cpds ( cpd photolyase ) or 6 - 4 products ( 6 - 4 photolyase ). a gene that expresses a protein with 6 - 4 photolyase activity in vitro, was recently cloned from high organisms ( arabidopsis thaliam, drosophila melanogaster, danio rerio, xenopus laevis and homo sapiens )

    目前已從高等生物擬南芥、鮐類、果蠅、人類和非洲爪蟾蜍屬中克隆到有（ 6 - 4 ）光裂合酶活性的基因，本研究從鹽生杜氏藻dunaliellasalina中克隆到（ 6 - 4 ）光裂合酶的基因，並將該基因在大腸桿菌中得以表達，這是首次在藻類中克隆到（ 6 - 4 ）光裂合酶基因，對光裂合酶的研究具有重要意義。
  15. These results show that coda gene cloned from e. coli can express specifically in the anther of both monocotyledon and dicotyledon plant species upon induction of 5 - fc, and the in vivo expression product of the gene can transform the 5 - fc used externally into 5 - fu, which leads to all or partial inactivity of anthers and / or pollens

    以上結果表明， coda基因在單子葉植物和雙子葉植物花藥中的特異性表達，能將外用的5 - fc在體內轉化為5 - fu ，導致花藥或花粉的部分和全部失活。
  16. The 600 bp and 800 bp pcr products were cloned into the pgem - t easy vector. their cdna sequences were determined with positive clones or purified pcr product. conclusion : compared the 600 bp pcr product with the amino acids sequence for the fibrinolysin metalloproteinase from the venom of agkistrodon acutus from the southern of anhui province, their homology is 90. 6 %

    結果：其中一對引物擴增得到一600bp產物；另一對引物擴增得到三條特異性的dna條帶，大小分別為1 . 5kb 、 1 . 3kb和800bp ，將600bp和800bpdna進行克隆及測序，並推導編碼的氨基酸序列。
  17. Researchers have since cloned cats goats cows mice pigs rabbits horses deer mules and gaur a large wild ox of southeast asia

    此後研究人員還克隆了貓山羊牛老鼠豬兔子馬鹿騾及白肢野牛東南亞地區一種大野牛。
  18. Researchers have since cloned cats, goats, cows, mice, pigs, rabbits, horses, deer, mules and gaur, a large wild ox of southeast asia

    此後研究人員還克隆了貓山羊牛老鼠豬兔子馬鹿騾及白肢野牛東南亞地區一種大野牛。
  19. Researchers have since cloned cats, goats, cows, mice, pigs, ra its, horses, deer, mules and gaur, a large wild ox of southeast asia

    此後研究人員還克隆了貓、山羊、牛、老鼠、豬、兔子、馬、鹿、騾及白肢野牛（東南亞地區一種大野牛） 。
  20. Leafy homologous gene cloned in maidenhair tree ginkgo biloba l

    同源基因的分離與克隆
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