coding region 中文意思是什麼
coding region
解釋
編碼區-
The coding region of the cdna of the phytoene desaturase gene was cloned by pcr and inserted into vector pbi121 containing intron - kanamycin
再生苗經過戍r和pcrs 。 : : hernbl 。 t分子檢測證明獲得了煙草轉基因植株。 -
Further we cloned full - length cdna of the ked like protein coding region via pcr amplification and confirmed its interaction with acam2 in yeast two - hybrid system
進一步通過rt . pcr克隆了ked樣蛋白編碼區全長cdna ，通過雙雜交驗證了全長ked樣蛋白與acamz的相互作用，結果為陽性。 -
As rna polymerase ii leaves a gene promotor to transcribe the coding region, it faces many obstacles, including nucleosomes
當rnap離開基因的啟動子轉錄編碼區時，遇到包括核小體在內的多種障礙。 -
( 3 ) giving a common stencil - plate for intelligent ascertaining model of other component in non - coding region
（ 3 ）為非編碼區其它組分的智能確定模型的建立提供了一個通用的模版。 -
For the svm classifier, we use the complete sequence of arabidopsis thaliana to research the coding region and the noncoding region in dna sequences
對svm採用了擬南芥全序列數據，對序列中的編碼區序列和非編碼區序列進行了研究。 -
All the isolates in the study of genotype vii could be recoganized by the new cutting site at nt 872 by re hinf i which is absent in the genetic supgroups viia, viib, and group vi. the amino acid sequences of the coding region of the f gene were deduced
由核昔酸序列推導了f蛋白高變區1一125位氨基酸（ aa ） ，通過分析比較發現了新的基因型和基因亞型與其他基因型毒株的差異還體現在aa位點的變化上。 -
The nature of all the three mutant alleles of this group is nonsense mutation disrupting the coding region of oxt gene
該組的三個突變體等位基因的突變都是無義突變，它們都破壞了。 xt基因的編碼區。 -
We cloned full - length cdna of ca coding region via pcr amplification, but a negative result was obtained when the interaction of full - length ca and ga was tested in yeast two - hybrid system
通過rt - pcr克隆了ca編碼區全長cdna ，但是在雙雜交系統檢測全長ca與g蛋白的相互作用時，結果為陰性。 -
A pair of degenerate primers were designed in the conserved domain which based on the alignment of acbf gene family in tobacco and arabidopsis. a 239 bp fragment was amplified by rt - pcr ( reverse transcription polymerase chain reaction ), which was used as a probe for screening tomato fruit ( pink stage ) cdna library. one positive clone containing entire coding region were isolated, which was identified as a new member of acbf family by blast server, and named as leacbf
根據genbank （中的擬南芥、煙草acbf家族成員序列比較的結果，在該基因的保守區設計簡並引物（ degenerateprimer ） ，以轉色期普通番茄果實的rna為模板，進行rt - pcr擴增，獲得239bp的擴增片段，以此片段作為探針篩選轉色期普通番茄果實cdna噬菌體文庫，獲得了包含全長編碼區的陽性克隆。 -
Methods : a set of oligonucleotide primers were designed and used to amplify the vh and vl gene from anti - hbsag fab antibodies screened from phage antibody library. the products were cloned into puc19 vector and their sequences were analysed. the vh and vl gene fragments were tethered by a peptide linker and a leader sequence coding region, with the leader sequence added at 5 " terminus of each gene ( l - vh - linker - vl ) and designated as l - scfv
方法以從噬菌體抗體庫中篩選獲得的抗hbsag的fab抗體基因為模板，分別擴增出其輕、重鏈可變區（ v _ l 、 v _ h ）基因，通過重組pcr方法將輕、重鏈可變區基因用連接肽（ gly _ 4ser ） _ 3的編碼序列連接，並引入前導肽編碼序列，構建具有l - v _ h - linker - v _ l結構的單鏈抗體基因。 -
The result demonstrates that the sequence of the coding region is 1167bp, encodes a protein of 389 amino acid
2kb左右的片段。核酸序列分析表明，該片段的編碼區長1167bp ，編碼389個氨基酸。 -
Hdgs induction by progeny containing a low copy number of the vector was also dramatically higher than induction by single copy vectors. the reduction of gfp protein and mrna abundance, and the presence of 21 - 26 nt sirnas could be simultaneously detected in silenced plants and the gfp coding region of these plants was methylated extensively
在發生沉默的植株體內，在gfp蛋白及其rna被不同程度降解的同時，可以檢測到21 - 26nt的小分子rna （ sirna ） ，同時gfp基因編碼區也受到顯著地甲基化修飾。 -
The coding region of cdna was cloned into procaryotic expression vector pet30a and overexpressed in e. coli bl21 ( de3 ). the cyclase proteins extracted from bacterial culture were found largely in the insoluble protein fraction
Cdna編碼區序列被克隆進原核表達載體pet - 30a ，並在大腸桿菌bl21 （ de3 ）中誘導表達，但過量表達的蛋白主要是以不溶性蛋白形式存在。 -
For prokaryotic expression, the coding region of the cdna of the phytoene desaturase gene was cloned by pcr and inserted into a prokaryotic expression vector pet - 21a ( + ). the gene was overexpressed in e. coli bl21 ( de3 ) and gave rise to a 63 kd mature protein in response to the iptg induction
用pcr方法擴增番紅花八氫番茄紅素脫氫酶（ pds ）編碼區序列，定向克隆到表達載體pet一藝1a （ + ）上，獲得重組質粒pet一21a （ + ） / cspds 。 -
Sequence analysis the cdna clone contains an 546bp orf ( open reading fragment ) encoding a predicted protein of 181 amino acids with molecular weight of 20. 7kd, 130bp 5 ' non - coding region and 152bp 3 " non - coding region including polyadenylation signal sequence aattaa and poly a tail. 3
棉花arf基因的序列分析核苷酸序列分析表明， gharf的全長為828bp ，其中含有一個從131bp到676bp間的546bp構成的一個完整開放閱讀框，它編碼181個氨基酸、分子量為20 . 7kd的蛋白質。 -
Hegf gene with his - tag at the end, which was derived from pet22 - egf, was in - frame fused to the carboxy - terminal of polyhedrin ( ph ) gene, which included the amino - terminal 116aa coding region. the polyhedrin - egf fusion gene ( named ph - egf ) was then cut out with ecorv and ecori, and was cloned between ecorv and ecori sites of pbacpak. 8 ( the result plasmid was named pbacph - egf and the ph - egf fusing gene was right under the control of ph promoter ). the pbacph - egf structure was verified with restriction enzymes digestion and pcr
從pet22 - egf質粒中分離出末端帶his - tag的egf基因，對位融合於多角體蛋白n端116個氨基酸基因序列的下游（命名為ph - egf ） ，並在兩段基因間設計了凝血酶xa蛋白酶切位點，經過酶切、測序等鑒定正確后，克隆至pbacpak8中，使ph - egf融合基因置於多角體蛋白（ polyhedrin ， ph ）基因啟動子控制之下，構建成重組轉移載體pbacph - egf 。 -
To allow secretion of the crylac protein into the intercellular space, potato proteinase inhibitor ii ( pinll ) signal peptide sequence was n - terminally fused to the crylac coding region to construct plasmid p3301ubisigac
運用pcr技術克隆了馬鈴薯蛋白酶抑制劑基因的信號肽序列，並將其分別連到crylac 、 gfp基因的5 』端，構建植物轉化載體p3301ubisigac和p3301ubisiggfp 。 -
Sequencing and genbank blasting demonstrated that these four had the same sequences and encoded part of arabidopsis choloroplast carbonic anhydrase ( ca ). the coding region contained 251 amino acid, from n terminal amino acid 86 to c terminus
到genbank進行序列比對，結果表明它們編碼擬南芥葉綠體碳酸酐酶（ ca ）基因，編碼區含有部分ca序列，從n端第86位氨基酸至c末端，共251個氨基酸。 -
Pcr products were inserted into pbluescriptsk using psti and xhoi. the element bearing resistance to streptomycin ( sm ) and spectinomycin ( sp ) was excised from php45 and inserted into gene coding region. the disrupted gene was isolated as a pstl and xhol fragment and cloned into prl271
首先將pcr擴增片段（ 1 . 5kb ）利用pst和xho插入常規克隆載體pbluescriptsk ，利用基因內部合適的酶切位點插入sp sm抗性片段，再利用pst和xho位點將已插入抗性基因的pcr片段，轉入整合載體prl271中獲得體外基因中斷，最後利用三親本雜交獲得體內突變。 -
We have created transgenic tobacco plants in which expression of the atnced3 coding region is under strong constitutive 35s cauliflower mosaic virus ( 35s ) promoter, stress - inducible rd29a promoter or stomotal - specific kst1 promoter by agrobacterium tumefaciens - mediated transformation
構建了組成型35s 、誘導型rd29a 、氣孔特異表達性kst1啟動子驅動atnced _ 3基因表達的真核表達載體，通過農桿菌介導的葉盤法轉化煙草，得到了轉基因植株。
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