competitive elisa 中文意思是什麼
competitive elisa
解釋
競爭性- competitive : adj 競爭的，競賽的。 competitive bidding system 招標制。 competitive examination 競爭考試。 compe...
- elisa : 埃莉薩
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Detection of antigen - binding affinity of soluble mg7 scfv elisa was adopted to examine the antigen - binding affinity of soluble mg7 scfv ; competitive elisa was performed to test the inhibitory ratio of soluble mg7 scfv to the binding affinity of mg7 mab with its relevant antigen
El舊a拐成惴現2個克隆的可容牲mg7seem明顯的抗原結合舌隊其a分別為0 832和0 912 ，均高出附性對照（ 0 -
Detection of antigen - binding affinity of mg7 recombinant phage antibody elisa was repeated to confirm the antigen - binding affinity of positive clones screened out in the former procedure ; these positive phages were examined by restriction analysis ( ecor i and hind iii ) ; competitive elisa was performed to test the inhibitory ratio of these positive clones to the binding affinity of mg7mab and its relevant antigen, the positive dones possessing apparent inhibitory effect were singled out for later use
X陽性克到駛知烘扳原kbbjg ）濁對陽性克隆進行限制隴臥賜析（ uwi和hedlll ）鑒定三用競爭elisatoljmg7重組噬菌體抗體性克隆對mg7單扶與其相應抗原結合忙的喇率，從中j ） ed出對mg7單抗與期眩抗原結合有抑余j效應的克隆用於進一涉研究。 -
By elisa analysis, inhibition of binding of clq with the c ! q receptors on u937 cells and competitive inhibition of binding of clq with aggregated immunoglobulin g b y selected phage clones, and dna sequencing, a number of similar, but not identical, sets of sequences of clq - binding clones were identified. the deduced amino acid sequences of selected 9 peptides are wyegpftlytwp, hwdpfslsayfp, ltqhnspffllp, tsnpfflwypqp, qtpfqlw, npfnwts, spfxlts, fltwldp and fstflyp. they show significant efficiency to inhibit the binding of clq with the clq receptors on u937 cells and / or aggregated immunoglobulin g, which suggest that the selected peptides contain the modeling epitopes of clq receptor to bind the collage - like region or igg to bind the head domain of clq
然後，採用噬菌體肽庫技術，以c1q為釣餌蛋白，從12肽庫和環7肽庫中親和篩選能與c1q結合的噬菌體克隆，經elisa 、 u937細胞配體結合抑制試驗、 aigg競爭抑制試驗及dna測序，獲得了9個具c1q抑制活性克隆的dna序列，其相應的氨基酸序列為： wyegpftlytwp 、 hwdpfslsayfp 、 ltqhnspffllp 、 tsnpfflwypqp 、 qtpfqlw 、 npfnwts 、 spfxlts 、 fltwldp 、 fstflyp ，它們可能模擬c1qr和或igg的c1q結合表位並抑制c1q的活性。 -
Part ii screening of tnfa mimotopes from phage display peptide library : based on the results of screening tnfa binding - peptides, we have tried to use neutral tnfa mcab j1d9 as target to screen tnfa mimotopes from c7c phage display peptide library, which may be another form of antagonist for tnfa, and the mimotopes were identified by sandwich elisa. after 3 rounds of screening, we got 9 phage clones identified as positive clones which can bind with mcab j1d9. we also identified the binding between mimotopes and tnf receptor by competitive elisa, and the results showed strongly binding. the amino acid sequence results shown three different sequences : c - rrpaqsg - c - nkhnrki - c and c - rgmsrki - c
在對噬菌體環七肽庫進行三輪親和性篩選后，隨機挑選20個噬菌體克隆， elisa鑒定出9個陽性克隆，經dna測序推出三種氨基酸序列： c - rrpaqsg - c 、 c - nkhnrki - c和c - rgmsrki - c ，其中優勢克隆序列為c - rrpaqsg - c ；鑒定結果顯示陽性克隆能夠與tnf受體結合，並且能夠阻斷tnf與受體的結合，提示篩選得到的環七肽克隆展示肽具有tnf的抗原性及與tnf受體結第一軍醫大學顧士學位論文合的特性，為tnfa表位模擬肽。 -
Polyclonal antibody to butachlor was prepared from rabbits immunized with butachlor - bsa conjugate, and a competitive elisa was developed for the detection of the butachlor a major component in a herbicide within the range of 110
Mg ml之間，且和化學結構相似的甲草胺和乙草胺無交叉反應。該方法也可用於檢測稻田水樣中丁草胺的含量。
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