creatinase 中文意思是什麼
creatinase
解釋
肌酶-
It is the optimal time for subjecting creatine to the medium when cultured to 12h and the concentration of creatine was 0. 75 %. creatine, sarcosine and choline chloride could induce the creatinase production and creatine was the optimal inducer, but creatinine and urea could not induce the creatinase production. 3 purification of creatinase the process of creatinase purification was performed as follows : first the enzyme was completely precipitated in the range of 40 - 80 % of saturation with ammonia sulfate fraction precipitation
最佳氮源為玉米漿和蛋白腖，最佳比例為2 ： 3 ，最佳濃度為1 . 6 ；加入其它碳源時有助於菌株穩定產酶； 100ml搖瓶的最佳裝液量為15ml ；肌酸、肌氨酸和氯化膽堿都能誘導菌株產酶，其中肌酸誘導產酶的效果最好，而肌酐和尿素不能誘導菌株產酶；誘導物肌酸的最適加入時間為接種培養12小時后，最適加入量為0 . 75 。 -
The paper deals with the isolation and identification of a creatinase - producing bacterium from soil, the conditions for production of creatinase, purification methods of creatinase and the purified enzyme characteristics
1 、產肌酸水解酶微生物的分離及鑒定本研究用恆化培養的方法從土壤中富集微生物，然後再用平板劃線分離。 -
Then enzyme was purified with a deae - cellulose ( 5. 5x50cm ) column, a toyopearl hw - 65 ( 5. 5 x 50cm ) column and a sephadex g - 200 ( 5. 5 x 80cm ) column. finally, the enzyme was purified for 10 folds with the recovery of 17. 4 %. page showed a single band for the purified creatinase
3 、肌酸水解酶的提純酶在硫酸銨飽和度為40 80之間完全沉澱，先後經過deae - cellulose離子層析柱、 toyopearlhw - 65疏水層析柱、 sephadexg - 200分子篩層析柱層析，最終使酶提純10倍，最終得率為17 . 4 。
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