culture cell 中文意思是什麼

culture cell 解釋
培養細胞
  • culture : n. 1. 教養；修養；磨煉。2. 文化，（精神）文明。3. 人工培養，養殖；培養菌，培養組織。4. 耕作；栽培；造林。vt. 使有教養。
  • cell : n 1 小室，單室；隔間，艙；〈詩〉茅舍；（單個的）蜂窩，蜂房。2 〈詩〉墓穴，墓。3 （大修道院附屬的...
  1. This paper introduced the application of biotechnology in rice genetics and breeding, including tissue culture, cell mutants selection, protoplast fusion, apomixis, molecular mark assisted breeding and genic transformation

    簡要綜述了生物技術在水稻遺傳育種中的應用，主要包括組織培養、細胞突變體的篩選、原生質體融合、無融合生殖以及分子標記輔助育種和轉基因技術等方面。
  2. It is an important that bacteria contaminated vaccine in the biologicals production. we collected 703 samples of cell culture, virus cultivation and harvest which were contaminated by bacteria during poliovaccine production within two years. we checked these samples by bacteriological method and antibiotics sensitivity tests were done. it shows that 1 ) the main contaminated bacteria come from staphylococci, bacilli and streptococci of environment in the poliovaccine production. 2 ） it is effect that antibiotics to contaminated bacteria are doxycycline, albiotic, prescription 2, cefotaxime na salt, gentamycin, neomycin, aureomycin and erythromycin

    在疫苗生產實踐中，細菌污染是影響疫苗質量和產量的關鍵性因素，筆者通過了兩年左右的時間，選取正常生產中零星細菌污染的細胞培養瓶、病毒培養瓶及收毒污染樣品等共703份，進行細菌學檢查，並對造成污染的主要細菌種類進行了各種抗菌藥物的耐藥性實驗，結果表明：我所脊灰疫苗生產中主要的污染威脅來自環境中的葡萄球菌，潛在威脅是桿菌和鏈球菌；強力黴素、林可黴素、配方2 、噻孢黴素鈉鹽、慶大黴素、新黴素、金黴素和紅黴素等抗生素對目前引起污染優勢細菌-葡萄球菌有明顯的抑菌效果，可作為疫苗生產后備抗菌手段參考
  3. According to the mechanism of block of development in vitro culture on early embryo of mammal and in vivo surroundings of early embryo, the paper states that requirement and utilization of nutrients during each cell stage of early embryo of mammal in vitro culture in order to search for in vitro culture condition and method to improve the development rate of blastosphere

    摘要從哺乳動物早期胚胎體外培養發育阻斷機理和早期胚胎的體內環境入手，闡述了胚胎體外培養各細胞階段胚胎對營養物質的需求，尋求合理的體外培養條件和方法，以便提高體外胚胎早期的囊胚發育率。
  4. The primary results showed : using m199 as diluents containing 20 % bovine serum, it is better to freeze the cells slowly freezing at fist then increase freezing speed ( for example, from 0 to - 6 freezing speed is about - 0. 05 a minute, from - 6 to - 40, freezing speed is about - 0. 5 a minute ), studies on effect of various concentration of dmso demonstrate that about 12. 5 % dmso gave the highest post - thaw percentage of viable cells. the concentration of bovine serum had no different effect on the percentage of the viable embryo cells of misgurnus auguillicaudatus. the embryo cells derived 6 from the later stage of blastula offish is more resistant to the cryogen than the cells of early stage of blastula. the cells preserved in liquid nitrogen at - 196 were thawed and cultivated, a few cells were found adhere to the surface of culture vessel when the percentage of viable cell was more than 30 %. the cells in only two culture vessels were found to proliferated and gave rise to many small morphologically undifferentiated cells

    研究初步表明:以細胞培養液m199 （含2既的小牛血清，常規量雙抗）為凍存稀釋液對泥鰍胚胎細胞冷凍保存宜採取先慢后快的方式（例如，從0一一6 ，凍存速度為一0 . 05 / min ，再以一0 . 5 / min的速度從一6一一40 ） ; dmso的保護效應濃度為12 . 506左右;小牛血清的濃度對泥鰍胚胎細胞的成活率影響不明顯;囊胚晚期細胞抗凍性比中早期強;通過對不同批次的凍存細胞解凍培養，解凍后成活率為30 %以上細胞培養數天後均有少數細胞貼壁，但只發現兩瓶培養細胞有明顯增殖現象產生許多未分化的小細胞。
  5. Poly ( lactic acid ) ( pla ) has been widely used as scaffolds for tissue engineering because of its good processability, good biocompatibility and suitable mechanical properties. but its catabolite would often induce erythrophlogosis. the preparation and properties of the pdlla / ha compound fiber and cell culture on the pdlla / ha unwoven meshes had research researched in this paper

    聚乳酸（ pla ）因其良好的生物相容性、生物可降解性以及良好的可塑性而被廣泛地應用於組織工程支架材料的研究，但是單一的聚乳酸長期在培養液或機體內因降解而易導致局部炎性反應。
  6. Synthetic cell and tissue culture media and components

    細胞和組織培養供應品及設備
  7. Development and application of animal cell culture medium

    動物細胞培養基的發展及應用
  8. Methods : cell culture in serum - free medium, indirect immunofluorescence cytochemistry were used

    方法：採用無血清細胞培養技術，間接免疫細胞化學染色法。
  9. Under suspension culture without cytokines, the putative eg clonies could be induced to form simple embryoid bodies. after long - time culture, these bodies began to differentiated into various cell types

    將消化分散的類eg細胞在無分化抑制因子的類eg培養基中重懸培養， 3 4天後，可見部分細胞團形成簡單的胚體。
  10. Spontaneous differentiation of es cell d3 line in vitro es cell d3 line via suspension culture can congregate small mass within 24 h, and large numbers of aggregates called embryoid bodies ( ebs ) gradually formed after 2 to 3 days of culture

    5 . es一d3細胞系的自主分化es一d3細胞懸浮培養， 24h后可聚集成小的細胞團，第2 ~ 3d時形成大量的類胚體（ ebs ） 。
  11. There is no different between the fibroblast feeder of mouse and the fibroblast feeder of goat according to blastoysts attached and icm proliferation. es - like cell develop embryoid body - like structure in suspension culture in vitro. es - like cells of goat differentiate into various kinds cell, egg : fibroblast cell, neurolike cell

    Es細胞在體外懸浮培養，形成類胚體，山羊類es細胞在無飼養層鋪有明膠的皿底上培養，可分化為多種類型細胞，如上皮細胞、成纖維細胞、神經細胞等。
  12. Section two the evaluation of biocompatibility of the acellular dermal matrix by the method of cell culture. the new born rat ' s epdermic cells were cultured with the acellular dermal matrix together as experiment group, while the epdermic cell were cultured simply as control. 24 hours later, under the invert microscope, the epidermic cells anchored well and transparent flat cells were observed in both groups. 7 days later, both cultured cells were taked out and fixed in 95 % ethanol, stained with hematoxylin and were observed under light microscope. many cleaved cells were observed in both groups. during cell culture, no pathogenic microganism was observed. so we considered the acellular dermal matrix was aseptic and had good biocompatibility. section three subdermal implantation of the acellular dermal matrix. 24 rats were used in the experiments. a piece of acellular dermal matrix ( 1. 5 x 1. 5cm2 ) was implanted beneath the dorsum skin flaps of each rat, 1 week, 2 weeks, 3 weeks and 4 weeks after implantation, 6 pieces of acellular dermal matrix were harvested and the size of implanted acellular dermal matrix were measured, the sections were used for he staining and observed under light microscope. the result were as folio wing : 1 - 2 weeks after implantation, the acellular dermal matrix began to adhere to the tissue around and turned red gradually ; 3 - 4weeks after implantation, the acellular dermal matrix adhered closely to the tissue around and could be recognized easily, 1 - 3 weeks after implantation, the size of implanted acellular dermal matrix had no statistical difference ( p > 0. 05 ). 4 weeks after implantation implanted acellular dermal matrix contracted ( p < 0. 05 ). under light microscope, l - 2weeks after implantation, the fibroblast cells infiltrated the acellular dermal matrix and a small amount endothelial cells of vessel and lympho - histiocytic cells infiltrated the acellular dermal matrix. 3 - 4 weeks after implantation, infiltrating blood vessels were evident. so we think that the acellular dermal matrix had low immunological reactions and could induce the infiltration of fibroblast macrophage cell and the endothelial cells of vessel

    結果如下：皮下包埋卜周者，無細胞真皮基質漸與周圍組織粘附，顏色由蒼白轉紅；皮下包埋3周者，無細胞真皮基質與周圍組織緊密枯附，盾晰葉辯；術后卜周，包埋的基質面積變化較包埋前無統計學差異o川0引，術后4周包埋的無細胞真皮基質面積較包埋前縮小j刃刀5 ） 。光鏡下術后卜周，宿主的淋巳組織細胞、成纖維細胞浸入生長，釉附在膠原纖維上，少量血管內皮細胞浸入基質；術后34周，無細胞真皮基質內較多的血管形成，故可認為無細胞真皮基質免疫原性低，能誘導宿主的成纖維細胞、巨噬細胞浸入生長，為一種新型的真皮替代物。第四部分無細胞真皮基質與自體斷層皮片復合移棺的研究， sd大鼠10隻，在其背部卜方造成全厚皮膚缺損的創面
  13. Thus, in order to investigate the developmental pathways not only involved in the regulation of growth and patterning, but also in the determination of cell lineages and differentiation, we utilized the fluorescent immunohistochemical methods, flow cytometry analysis sorting ( facs ) and molecular methods to investigate the developmental law of mammary gland at the different developmental stages, distribution of the stem cells in mammary gland, the methods of isolation, culture and evaluation for the stem cells, the multipotent abilities in vivo and in vitro, and the efficient cultural system for stem cells enriched in vitro. the results showed below : 1

    我們以小鼠為模型，運用組織化學、免疫熒光組織（細胞）化學、流式細胞儀分選方法（ facs ）以及分子生物學手段，研究了小鼠乳腺的發育規律：小鼠乳腺組織中類乳腺幹細胞：小鼠乳腺細胞的分離、培養以及類乳腺幹細胞的鑒定；小鼠類乳腺幹細胞分化的潛能；小鼠乳腺類腺體體外短期培養富集類乳腺幹細胞體系的優化等。研究結果表明： 1
  14. Fibroblast cell of adult mouse culture in vitro

    成年小鼠成纖維細胞體外培養
  15. Part 1 : the culture and identification of es - d3 cells and the study of the efficiency of eb formation from es cells when grown on mef feeder layer in es culture medium or cultured in es culture medium supplemented with lif 1000u / ml, es - d3 cells being used in our experiments formed normal clones, expressed akp and kept their normal karyotype over many passages. the in vitro and in vivo differentiation experiments showed that es - d3 cells could differentiate into variety of cell types derived from three primary germ layers

    結果顯示： eso3細胞在小鼠胚胎成纖維細胞上和或含白血病抑制因於億f ）的es細胞培養液中形成典型的胚胎幹細胞克隆，堿性磷酸酶染色結果為強陽性，具有正常二倍體核型以及具有在體內外分化為三個胚層來源的組織細胞的潛能，而且具有形成種系嵌合動物的能力。
  16. Different from mammals, the early embryos of fish can not be preserved for the long period at the very low temperature ( - 196 ). therefore, three methods were usually applied to cryogenic preservation of the fine and rare species of fish : 1 ) perserving fish spermatozoon in cryogenic condition. researchers have had systematically studied on this technique for many years, and this technique has been utilized in application and made a lot of effects ; 2 ) combining with the techniques of cell engineering ( nuclear transplantation and electric fusion etc. ), and through the process of culturing histiocyte of fish, cryopreservation and re - culture after thawing, carrying out somatic cell breeding of fish. the past studies showed that the nucleolus of somatic cells of fish have totipotency

    多年來，國內外學者對各種魚類精液的冷凍保存進行了大量的系統研究，目前這項技術已達到實用水平，並日益發揮作用；二是對魚類培養的組織細胞冷凍保存，通過魚類細胞的培養、超低溫凍存、解凍后再培養過程，結合細胞工程技術（如核移植、電融合等）進行體細胞育種；大量的研究結果表明魚類體細胞核具有發育的全能性，隨著細胞培養技術、細胞工程技術日益發展成熟，完全具備實現魚類物種種質長期保存的理論基礎和技術條件。
  17. A pair of primers were chemically synthesized based on the cdna sequence of hog cholera virus strain brescia and used to amplify the cdna fragments of envelope glycoprotein e2 gene of hclv which coding the major protective antigen by reverse transcription - polymerase chain reaction ( rt - pcr ) from total intact rna extracted from infected calf testicle cell culture by hclv

    以豬瘟兔化弱毒犢牛睪丸細胞毒（ hclv ）中提取的細胞總rna為模板，以rt - pcr技術，擴增出了完整e2基因的cdna片段。經電泳、酶切分析，證實了所擴增片段為e2基因特異性片段。
  18. Endothelial cell culture in vitro from umbilical blood

    臍血內皮細胞的體外培養
  19. Abstract : adopting the serum - free and animal - source - free medium domestication express cell efficiently, setting up to express system efficiently, suspending culture cell, can raise the cell density in the scale turn the production, strengthen the cell vitality, control cell to propagate level, extension cell culture period, increase the target protein of yield, raise product quality, simplification of produces technics, reduce production cost, then raising the efficiency that the scale turns culture

    提要：採用無血清無動物組分培養基馴化高效表達細胞，構建高效表達系統，懸浮培養細胞，可以在規模化生產中，提高細胞密度，增強細胞活力，控制細胞增殖水平，延長細胞培養周期，增加目標蛋白的產量，提高產品質量，簡化生產工藝，降低生產成本，進而提高規模化培養的效能。
  20. In the course of culture, cell density, content of chlorophyll - a, biomass, content of intracellular protein and intracellular carbohydrate were determined and analyzed, and some other correlative parameters were calculated and compared

    在藻生長過程中，對其細胞數、葉綠素a 、生物量、細胞內蛋白質和糖含量進行測定和分析，並對其它相關參數做了計算和比較。
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