deae-cellulose 中文意思是什麼
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In this paper, by shorting the length of deae - cellulose column and increasing the ph of elution solution, one kind of go isozyme was purified rapidly from green leaves of spanictu brassica parachinensis bailey and vigna unguiculatew. ssp. sesquipedalis ( l )
本文通過一種改進后的方法，即通過縮短deae - cellulose - 52柱的長度，以及提高洗脫液的ph ，可在9h內從菠菜、菜心和豆角綠葉中純化go同工酶。 -
The xerocomus spadiceus lectin, xsl, was isolated from extracts of fruiting bodies of the mushroom xerocomus spadiceus using a procedure that involved ( nh4 ) 2so4 precipitation, anion exchange chromatography on deae - cellulose, affinity chromatography on affi - gel blue gel, cation exchange chromatography on cm - cellulose, and gel filtration by fast protein liquid chromatography on superdex 75
從磚紅絨蓋牛肝菌（ xerocomusspadiceus ）子實體粗提物中，經過deae -纖維素陰離子交換層析、 affi - gelbluegel親和層析、 cm -纖維素陽離子交換層析和superdex75fplc凝膠過濾，純化了磚紅絨蓋牛肝菌凝集素。 -
Then enzyme was purified with a deae - cellulose ( 5. 5x50cm ) column, a toyopearl hw - 65 ( 5. 5 x 50cm ) column and a sephadex g - 200 ( 5. 5 x 80cm ) column. finally, the enzyme was purified for 10 folds with the recovery of 17. 4 %. page showed a single band for the purified creatinase
3 、肌酸水解酶的提純酶在硫酸銨飽和度為40 80之間完全沉澱，先後經過deae - cellulose離子層析柱、 toyopearlhw - 65疏水層析柱、 sephadexg - 200分子篩層析柱層析，最終使酶提純10倍，最終得率為17 . 4 。 -
Calmodulin - dependent cyclic nucleotide phophodiesterase was prepared from bovine brain by two - step deae - cellulose column chromatography
摘要通過兩次離子交換柱層析，從牛腦中制備鈣調素依賴性的環核苷酸磷酸二酯酶。 -
Purification and characterization of phytase from a. niger an 01001 a. niger an01001 was inoculated on solid media and cultivated at 30 for 5 days. proteins were extracted from solid - state fermentation with 50mm acetate buffer ( ph5. 5 ). the molecule weight of the phytase protein was determined as about 78kd by sds - page. the purification procedures include ammonium sulfate precipitation, deae - cellulose ion - exchange chromatography, gel electrophoresis and electroelution
3 .植酸酶的分離純化及其性質研究黑麴黴ano1001經固體發酵，用緩沖液抽提后，經硫酸按沉澱， deae一纖維素離子交換層析，聚丙烯酞胺凝膠電泳和電洗脫等純化步驟獲得的植酸酶，用sds一page檢測為一條均一譜帶，其分子量約為78kd 。 -
3. 2. 1. 4 ) which was prepared by precipitation of the water extract of the culture of aspergillus niger with ammonium sulfate and desalted by sephadex g - 25, and was further fractionated by two steps of deae - toyopearl 650m and one step of poros 20pi chromatography. the other was a p - glucosidase ( ec. 3. 2. 1. 21 ) which was prepared by the above g - 25 fractions and was further fractionated by two steps of deae - toyopearl 650m chromatography. the specific activity of the endoglucanase with sodium carboxymethyl cellulose was estimated to be 433. 38 hj / mg
-葡萄糖苷酶對水楊素的比活力為597 . 12iu mg ，並對其專一，不能水解棉花和羧甲基纖維素鈉；分子量為117 . 5kda ，加dtt後分子量不變；該組分最適ph和溫度分別為4 . 5和70 ，在ph5 . 0 、 50下對水楊素鈉的米氏常數km為3 . 73mg ml ，最大反應速度vm為0 . 088mg葡萄糖（ ml ? min ） ；與文獻中從黑麴黴中分離的-葡萄糖苷酶比較后發現，該組分是一個新的-葡萄糖苷酶。 -
The isolation and purification of dnaase in the earthworm the earthworm dnaase was purified from the tissue extract of earthworm by denaturing the protein with low ph buffer, high temperature, ammonium sulfate precipitation, deae - cellulose ( de52 ) chromatography and ultra - filter membrane
雙胸蚓組織中dna酶的分離純化雙胸蚓組織粗提取液經過選擇性酸變性、選擇性熱變性、硫酸按分段鹽析、 deae一纖維素（ de52 ）柱層析、超濾膜分級分離后得到一個電泳純的dna酶。 -
An active metabolite was obtained by purification with precipitated by ethanol, sephadex g - 25 gel, deae - cellulose ion exchange resin and silica gel column chromatography
經乙醇（ 95 ）沉澱、 sephadexg - 25凝膠層析、 deae -纖維素離子交換層析和硅膠層析純化，得到抑制黑麴黴生長的單一組分。 -
But the whole level of serum titer in combined vaccine group was higher than others. igg was extracted by salting out with ammonium sulfate and purified by ion exchange chromatography with deae cellulose
對分離到的血清用飽和硫酸銨鹽析法提取igg ，並用deae纖維素離子交換層析法對提取的igg進行純化。 -
To isolate and purify dnaase in the earthworm first, the tissue extract of earthworm was prepared by dissolving the earthworm with sucrose and denaturing the protein with low ph buffer. then dnaase was purified by denaturing the protein with higher temperature. the following steps were ammonium sulfate precipitation, deae - cellulose ( de52 ) chromatography and filtration by ultra - filter membrane
雙胸蚓組織中dna酶的分離純化採用蔗糖溶解雙胸蚓，並選擇性酸變性制備雙胸蚓組織粗提取液，再經選擇性熱變性、硫酸銨分段鹽析、 deae ?纖維素（ de52 ）柱層析及超濾膜分級分離對雙胸蚓組織中dna酶進行分離純化。 -
3. successful purification of the crude ha was achieved by anion exchange resin deae - cellulose column chromatography using 0. 6mol / lnacl solution as an eluant
3 .採用陰離子樹脂deae一纖維素對透明質酸粗品進行純化，當naci為0 . 6mol / l時，蛋白質含量較低。 -
The enzyme was purified from cell extract by ammonium sulfate fractionation ( 30 % - 80 % saturation ) and consecutive column chromatography using deae - cellulose and sephadex g - 100. the sod activity for purified enzyme could reach 4966 iu / mg proteins, and the molecular weight of the sod was about 20 kda which determined by sds - page electrophoresis. when the non - denaturing polyacrylamide gel stained with substrate for sod in the present of 0. 2mmol / l kcn or 0. 5 mmol / l h2o2, the active band was still showed at the same position, which indicated the sod could not be inhibited by the treatments with kcn or h2o2 and it was the mn sod
通過sds一聚丙烯酸胺凝膠電泳可知該sod酶的分子量約為20kda .在非變性聚丙烯酞胺凝膠（ pagb ）電泳后，在凝膠son顯色反應液中加入0 . 2耐01 / lkcn或0 . 5inmol / lh202 ，凝膠sod顯色反應后在原來的sod酶部位仍然含有sod活性帶，這表明利用kcn和h20 :處理並不能抑制500的活性，該sod屬于mn一sod 。 -
The precursor of cholesterol oxidase of atcc14811 was predicted with a calculated mr of 60020. the cholesterol oxidase produced by streptomyces griseus atcc14811 was purified from the culture broth by procedures including steps for preparation of crude enzyme solution, ammonium sulfate fraction and column chromatography on deae - cellulose
Chog與chob （ brevibacteriumsterolicum ）的氨基酸同一性和相似性分別為53和70 ，根據推導的氨基酸序列chog蛋白質前體的分子量為60020 ， chogn端區域顯示信號肽特徵。 -
The crude extraction of dry pea seed, which was obtained through marination, homogenization, filtration, centrifugation and other methods, was precipitated by 50 mmol / l ( final concentration ) mgq2. the pellet was chromatographed on aca ^ gel filtration and deae - cellulose 52 ( de 52 ) anion - exchanger. single eluting peak containing ferritin was obtained finally
2 、豌豆種子鐵蛋白的純化豌豆種子經浸泡、勻漿、過濾、離心等操作得到豌豆鐵蛋白粗提液，再用終濃度為50mmol / l的mgcl _ 2鹽析等處理后，經aca _ （ 22 ）凝膠過濾柱層析和deae -纖維素52 （ de52 ）陰離子交換柱層析等方法進行純化，得到單一的豌豆鐵蛋白洗脫峰。 -
In this optimal condition, p450nor was expressed massively. the expressed product was then purified by deae - cellulose chromatography. the purified expressed protein showed one band in sds - page and the purification attained anticipative purpose
在此條件下，大規模誘導表達重組細胞色素p450nor ，經deae纖維素色譜柱純化， sds - page分析表明，純化的目的蛋白基本上為單一譜帶，純化達到預期效果。
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