deae 中文意思是什麼

deae 解釋
2-二乙氨基乙醇
  1. Fourthly, according to the activity, collect, dialyse, freeze, dry respectively the sod protein through deae - sepharose column chromatog - raphy ; process the sod protein through sephacryl s - 200 column chromatography with the preceding method. at last, process the pure sod protein with same functional enzyme electrophoresis and active dye, isoelectric focusing electrophoresis and sds - page

    將粗酶液過deae -瓊脂糖柱層析，得三個活力峰，分別收集、透析、乾燥濃縮后；再上sephacryls - 200凝膠柱層析，按與deae -瓊脂糖柱層析后同樣的方法收集處理。
  2. 6 - phosphogluconate dehydrogenase ( 6 - pgadase, ec 1. 1. 1. 44 ) was isolated by homogenate, ammunium sulfate fractionation, deae - sepharose chromatography, blue - sepharose affinity chromatography and gel filtration with sephadex g - 200 from bacillus subtilis, and some properties of the enzyme had been studied. a 113. 8 - fold purification was obtained with a 8. 2 % yield. the purified enzyme moved as a single electrophoretic band in page

    將枯草芽孢桿菌超聲波破壁后的粗提取物進行分段鹽析、 deae - sepharose陰離子交換柱層析， blue - sepharosecl - 6b特異結合柱層析和sephadexg - 200凝膠過濾等純化步驟，得到聚丙烯酰胺凝膠電泳為單一蛋白區帶，比活為1 . 46u mg的酶制劑。
  3. In this paper, by shorting the length of deae - cellulose column and increasing the ph of elution solution, one kind of go isozyme was purified rapidly from green leaves of spanictu brassica parachinensis bailey and vigna unguiculatew. ssp. sesquipedalis ( l )

    本文通過一種改進后的方法，即通過縮短deae - cellulose - 52柱的長度，以及提高洗脫液的ph ，可在9h內從菠菜、菜心和豆角綠葉中純化go同工酶。
  4. The ps ii native fractions ( 20 % and 30 % ) were loaded onto a deae column. the fraction eluted with 150 mm nacl was presented dcip reduction activity and was highly depleted in chi c and xanthophylls, and as such could be considered a ps ii core complex

    對于有dcip光還原活性的20和30層帶的復合物，進一步deae離子交換層析純化。 150mmnacl洗脫純化后的樣品經過熒光激發光譜測定發現，已經去除了葉綠素c和墨角藻黃素，並且仍然具有dcip的光活性，分析是ps核心復合物。
  5. The xerocomus spadiceus lectin, xsl, was isolated from extracts of fruiting bodies of the mushroom xerocomus spadiceus using a procedure that involved ( nh4 ) 2so4 precipitation, anion exchange chromatography on deae - cellulose, affinity chromatography on affi - gel blue gel, cation exchange chromatography on cm - cellulose, and gel filtration by fast protein liquid chromatography on superdex 75

    從磚紅絨蓋牛肝菌（ xerocomusspadiceus ）子實體粗提物中，經過deae -纖維素陰離子交換層析、 affi - gelbluegel親和層析、 cm -纖維素陽離子交換層析和superdex75fplc凝膠過濾，純化了磚紅絨蓋牛肝菌凝集素。
  6. Then enzyme was purified with a deae - cellulose ( 5. 5x50cm ) column, a toyopearl hw - 65 ( 5. 5 x 50cm ) column and a sephadex g - 200 ( 5. 5 x 80cm ) column. finally, the enzyme was purified for 10 folds with the recovery of 17. 4 %. page showed a single band for the purified creatinase

    3 、肌酸水解酶的提純酶在硫酸銨飽和度為40 80之間完全沉澱，先後經過deae - cellulose離子層析柱、 toyopearlhw - 65疏水層析柱、 sephadexg - 200分子篩層析柱層析，最終使酶提純10倍，最終得率為17 . 4 。
  7. Calmodulin - dependent cyclic nucleotide phophodiesterase was prepared from bovine brain by two - step deae - cellulose column chromatography

    摘要通過兩次離子交換柱層析，從牛腦中制備鈣調素依賴性的環核苷酸磷酸二酯酶。
  8. Purification and characterization of phytase from a. niger an 01001 a. niger an01001 was inoculated on solid media and cultivated at 30 for 5 days. proteins were extracted from solid - state fermentation with 50mm acetate buffer ( ph5. 5 ). the molecule weight of the phytase protein was determined as about 78kd by sds - page. the purification procedures include ammonium sulfate precipitation, deae - cellulose ion - exchange chromatography, gel electrophoresis and electroelution

    3 .植酸酶的分離純化及其性質研究黑麴黴ano1001經固體發酵，用緩沖液抽提后，經硫酸按沉澱， deae一纖維素離子交換層析，聚丙烯酞胺凝膠電泳和電洗脫等純化步驟獲得的植酸酶，用sds一page檢測為一條均一譜帶，其分子量約為78kd 。
  9. - acetolactate decarboxylase is purifed from cell extract by 50 % - 80 % ammonium sulfate - fractionation, 50, 2min heat treatment and deae - sepharose fast flow column chromatography, which we study the different ph and different buffer of deae - sepharose fast flow column chromatography and conclude ph 6

    對其酶學性質進行了研究。 -乙酰乳酸脫羧酶經50 80硫酸銨分級沉澱、 50 ， 2min熱處理、 deae - sepharosefastflow離子交換柱層析方法分離純化。
  10. - acetolactate decarboxylase are widely found among bacterial strains but not in other groups of organisms. the enzyme has been demonstrated to be effective for removal of acetolactate and widely used in beer product. in this paper, - acetolactate decarboxylase from bacillus subtilis was purifed to homogeneity from cell extract by ammonium sulfate - fractionation, heat treatment, deae - sepharose fast flow column chromatography

    本文對來源於枯草芽孢桿菌（ bacillussubtilis ） 3226 - 5的-乙酰乳酸脫羧酶經硫酸銨分級沉澱、熱處理、 deae - sepharosefastflow離子交換柱層析等分離純化步驟，得到sds - paeg電泳純，通過n末端氨基酸序列分析驗證酶蛋白的純度。
  11. 3. 2. 1. 4 ) which was prepared by precipitation of the water extract of the culture of aspergillus niger with ammonium sulfate and desalted by sephadex g - 25, and was further fractionated by two steps of deae - toyopearl 650m and one step of poros 20pi chromatography. the other was a p - glucosidase ( ec. 3. 2. 1. 21 ) which was prepared by the above g - 25 fractions and was further fractionated by two steps of deae - toyopearl 650m chromatography. the specific activity of the endoglucanase with sodium carboxymethyl cellulose was estimated to be 433. 38 hj / mg

    -葡萄糖苷酶對水楊素的比活力為597 . 12iu mg ，並對其專一，不能水解棉花和羧甲基纖維素鈉；分子量為117 . 5kda ，加dtt後分子量不變；該組分最適ph和溫度分別為4 . 5和70 ，在ph5 . 0 、 50下對水楊素鈉的米氏常數km為3 . 73mg ml ，最大反應速度vm為0 . 088mg葡萄糖（ ml ? min ） ；與文獻中從黑麴黴中分離的-葡萄糖苷酶比較后發現，該組分是一個新的-葡萄糖苷酶。
  12. The isolation and purification of dnaase in the earthworm the earthworm dnaase was purified from the tissue extract of earthworm by denaturing the protein with low ph buffer, high temperature, ammonium sulfate precipitation, deae - cellulose ( de52 ) chromatography and ultra - filter membrane

    雙胸蚓組織中dna酶的分離純化雙胸蚓組織粗提取液經過選擇性酸變性、選擇性熱變性、硫酸按分段鹽析、 deae一纖維素（ de52 ）柱層析、超濾膜分級分離后得到一個電泳純的dna酶。
  13. Under these conditions the fibrinolytic activity of the supernatant can reach 820 urokinase units per milliliter broth. ba - dfe was purified from the supernatant of b. amyloiquefaciens dc - 4 culture broth by ammonium sulfate precipitation, ion - exchange chromatography on cm - and deae - sepharose fast flow, hydrophobic interaction chromatography on phenyl sepharose 6 fast flow and gel filtration on sephadex g - 50. the purified enzyme displayed thermophilic, hydrophilic and strong fibrinolytic activity

    通過硫酸銨分級沉澱、 cm - sepharosefastflow和deae - sepharosefastflow離子交換層析、 phenylsepharose6fastflow疏水層析和sephadexg - 50凝膠過濾等方法，從解澱粉芽孢桿菌dc - 4的發酵液中分離純化出電泳純的ba - dfe 。
  14. An active metabolite was obtained by purification with precipitated by ethanol, sephadex g - 25 gel, deae - cellulose ion exchange resin and silica gel column chromatography

    經乙醇（ 95 ）沉澱、 sephadexg - 25凝膠層析、 deae -纖維素離子交換層析和硅膠層析純化，得到抑制黑麴黴生長的單一組分。
  15. But the whole level of serum titer in combined vaccine group was higher than others. igg was extracted by salting out with ammonium sulfate and purified by ion exchange chromatography with deae cellulose

    對分離到的血清用飽和硫酸銨鹽析法提取igg ，並用deae纖維素離子交換層析法對提取的igg進行純化。
  16. The purification of this halocin was achieved by combination of tangential flow filtration ( tff ), sephadex g50 and deae - sepharose chromatography. the n - terminal amino acid sequence was also determined by edman degradation

    Halc8對大多數的極端嗜鹽古菌（包括部分嗜鹽堿古菌）有抑制作用，但對細菌則無明顯的抑制作用。
  17. To isolate and purify dnaase in the earthworm first, the tissue extract of earthworm was prepared by dissolving the earthworm with sucrose and denaturing the protein with low ph buffer. then dnaase was purified by denaturing the protein with higher temperature. the following steps were ammonium sulfate precipitation, deae - cellulose ( de52 ) chromatography and filtration by ultra - filter membrane

    雙胸蚓組織中dna酶的分離純化採用蔗糖溶解雙胸蚓，並選擇性酸變性制備雙胸蚓組織粗提取液，再經選擇性熱變性、硫酸銨分段鹽析、 deae ?纖維素（ de52 ）柱層析及超濾膜分級分離對雙胸蚓組織中dna酶進行分離純化。
  18. Without ammonium sulphate fractionation and dialysis, the supernatant of crude extract was directly loaded on deae - sepharose cl 6b column equilibrated by phosphate buffer ( 50mmol / l, ph7. 8 ), and the enzyme fraction was not absorbed on the column but impurities were absorbed

    粗酶液無需硫酸銨沉澱及透析，即可引入磷酸緩沖液（ 50mmol l ， ph7 . 8 ）預平衡的deae - sepharosecl6b柱，上柱後用平衡緩沖液洗至基線穩定。 afpga不被吸附而直接流出。
  19. 3. successful purification of the crude ha was achieved by anion exchange resin deae - cellulose column chromatography using 0. 6mol / lnacl solution as an eluant

    3 .採用陰離子樹脂deae一纖維素對透明質酸粗品進行純化，當naci為0 . 6mol / l時，蛋白質含量較低。
  20. The brine shrimp he was prepared and purified by 67 % ammonium sulfate precipitation, deae - sepharose fast flow ion - exchange chromatography, and sephacryl column gel - filtration, and its biochemical and enzymological properties were identified in this study. it was found that the deduced molecular weight of he in sds - page is about 98. 5 kda, and its proteolytic activity was optimized at ph of 7. 5 - 8. 5 and at temperature of 40, respectively

    我們利用67硫酸銨沉澱、 deae - sepharosefastflow陰離子交換柱層析和sephacryl凝膠過濾柱層析，並以酪蛋白為其蛋白酶水解活性的檢測底物、以卵膜為其卵殼裂解活性的特異性底物，從鹵蟲胚胎孵化液中分離純化出了鹵蟲的孵化酶分子，其在sds - page電泳中的分子量約為98 . 5kda 。
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