degenerate pcr 中文意思是什麼

degenerate pcr 解釋
退化性聚合鏈反應
  • degenerate : vi 1 腐化，墮落，頹廢。2 衰敗；【生物學】退化 （to）；【生理】 變質。adj n 1 腐化的，墮落的，頹廢...
  • pcr : PCR ＝polymerase chain reaction 【生物化學】聚合酶聯鎖反應〈此項技術可用以復制犯罪現場發現的DNA小樣，從而有助於破案〉。
  1. Degenerate oligonucleotides to highly conserved regions of cucumis melo 1 - aminocyclopropane - 1 - carboxylic acid ( acc ) oxidase gene were used to prime the amplification of fragment of 128bp by ploymerase chain reaction ( pcr ) in samples of genomic dna from fruit of cucumis melo l. cv hetao flesh, which was cloned into plasmid vector pmd - 18 - t. the clon of antisense orientation were selected, and it was inserted downstream of camv35s promoter and enhancer " " of tmv into the plant expression vector pbinyxw, antisence expression vector pbinya was constructed. at the base that pollination and fertilization of cucumis melo l. cv hetao was studied, using pollen tube pathway transformate cucumis melo l. cv hetao, 76 fruit had been obtained, moreover, hardness and content of sugar were analysed

    本實驗以河套蜜瓜果肉基因組dna為模板，用甜瓜acc氧化酶基因特異寡核苷酸鏈為引物進行pcr擴增，得到128bp的擴增產物。將得到的擴增產物克隆到質粒載體pmd - 18 - t上，篩選反向克隆，然後將其反向構建到植物表達載體pbinyxw的camv35s啟動子和tmv增強子「 」的下游，構建成反義表達載體pbinya 。並在對河套蜜瓜授粉受精生物學研究的基礎上，通過花粉管通道法轉化河套蜜瓜，共獲76顆瓜，並進行了硬度和含糖量的分析。
  2. Pcr amplification using 2 degenerate primers for nitrogenase fe protein gene was performed with chromosomal dna isolated from the 29 isolates. the result suggested that a nifh amplicon of 323 nucleotides was detected in 7 isolates and the 7 isolates are c4 c5 g1 g2, w5 t1 and t7. these pcr amplified fragments were cloned, and sequenced

    首先利用芽孢桿菌中芽孢的抗熱性將土壤溶液在100沸水中煮10 - 15分鐘，然後用選擇性無氮培養基進行初篩得到29株菌落形態不同的菌株；接著用固氮酶結構基因nifh的特異性引物對這29株菌進行pcr擴增，結果表明其中7個菌株具有nifh基因，這7個菌株的編號依次為： c4 、 c5 、 g1 、 g2 、 w5 、 t1和t7 。
  3. Using rt - pcr with two degenerate primes designed from conserved amino acids of cdpk kinase and autoinhibitory domains, three 618 bp cdna fragments ( 618 - 1. 618 - 2 and 618 - 3 ) were also isolated from broad bean leaves, and a partial cdna vjcpk2 lacking 5 " end was isolated from broad bean leaves with two gene - specific primers designed according to the 618 - 1 sequence

    用rt - pcr技術，以cdpks激酶區和連接區的保守氨基酸設計的一對簡並引物，從蠶豆葉片中還擴增到了3個618bp （ 618 - 1 、 618 - 2和618 - 3 ）的cdna片段；用race技術，以618 - 1序列設計的一對基因特異性引物，克隆到了還缺少5 』末端的cdna片段vfcpk2 。
  4. By using inverted microscope, it was observed that dunaliella salina of different growth stages after the high osmotic shocks can live in the medium with nacl concentration between 0. 1m and 5. 0m, but its growth status and period showed differently. the optimal concentration for the growth of dunaliella salina was 0. 5 - 1. 5m, and this organism could stand a variety range of osmotic shock. enolase gene, the anti - adversity gene of d. salina, was cloned by modified degenerate pcr technique

    通過倒置顯微鏡觀察生長在不同鹽濃度，不同生長時期，以及經不同滲透壓震動的鹽藻，四川大學博士學位論文發現其在o . im一5 . omnaci培養基中均能正常生長，但其生活狀態及生長周期有所不同，其最適生長naci濃度為0 . 5一1 . 5m ，還能適應各種高滲及低滲震動。
  5. Then, a piece of degenerate primer was designed according to the conserved amino acids of glycine betaine abc transporter system glycine betaine - binding protein as a reversed primer. combined with the opuaa - up, a 2. 3 kb fragment was obtained through pcr. blast result showed a fragment which contained the partial opuaa, the whole opuab and partial opuac sequences were obtained

    再次，根據甘氨酸甜菜堿atp轉運系統底物結合蛋白的氨基酸保守序列設計下游簡並引物，與atp結合蛋白的上游簡並引物組合，經pcr擴增獲得2 . 1kb的條帶，測序后通過blast比較，結果顯示獲得atp結合蛋白基因的部分序列、通透酶的全部編碼序列和部分甘氨酸甜菜堿結合蛋白基因的核苷酸序列。
  6. In this research two full - length cdnas have been cloned by a combination of rt - pcr and 3 " - is1 - race with synthesized degenerate primers from young leaves of vicia faba l., pichia methanolica high - level expression systems of the genes have been constructed, and the milligram expressed protein was purified using probond resin purification system, which may result in further identification of the function of the aba binding protein. the full - length cdna of abp370 fragment is 3449 bp long and has an open reading frame of 2304 bp encoding 768 amino acids with 876 bp long 5 ' - utr, 369 bp long 3 ' - utr and poly ( a ) tail. the full - length cdna of abp640 fragment is 1012 bp long and has an open reading frame of 780 bp encoding 260 amino acids with 88 bp long 5 ' - utr, 144 bp long 3 " - utr and poly ( a ) tail

    3 - 5 - race擴增片段序列分析結果表明， abp370擴增片段的全長cdna為3449核苷酸，其中5非翻譯區為876個核苷酸， 3非翻譯區為369個核苷酸並末端帶poly （ a ）尾巴，從起始密碼子atg至終止密碼子tga ，含有一個編碼768個氨基酸殘基的開放閱讀框架（ 2304bp ） ； abp640擴增片段的全長cdna為1012核苷酸，其中5非翻譯區為88個核苷酸， 3非翻譯區為144個核苷酸並末端帶poly （ a ）尾巴，從起始密碼子atg至終止密碼子taa ，含有一個編碼260個氨基酸殘基的開放閱讀框架（ 780bp ） 。
  7. A pair of degenerate primers were designed in the conserved domain which based on the alignment of acbf gene family in tobacco and arabidopsis. a 239 bp fragment was amplified by rt - pcr ( reverse transcription polymerase chain reaction ), which was used as a probe for screening tomato fruit ( pink stage ) cdna library. one positive clone containing entire coding region were isolated, which was identified as a new member of acbf family by blast server, and named as leacbf

    根據genbank （中的擬南芥、煙草acbf家族成員序列比較的結果，在該基因的保守區設計簡並引物（ degenerateprimer ） ，以轉色期普通番茄果實的rna為模板，進行rt - pcr擴增，獲得239bp的擴增片段，以此片段作為探針篩選轉色期普通番茄果實cdna噬菌體文庫，獲得了包含全長編碼區的陽性克隆。
  8. Detection of lily mottle virus by rt - pcr using special primers and degenerate primers

    應用特異引物和簡並引物檢測百合斑駁病毒
  9. Two degenerate primers were designed and synthesized according to the highly conservative sequences among the known - glc genes. a cdna fragment of 208bp was amplified by rt - pcr, which was subsequently cloned into pmd18 - t vector for sequencing analysis

    利用genbank中已經登錄的其它植物中該酶基因的保守序列，設計一對簡並引物，採用rt - pcr技術，從茶樹擴增出208bp的cdna片段。
  10. With a pair of degenerate primers designed according to the conserved region among the known cdna sequences of five rodents, a cdna fragment was generated by rt - pcr from total rna isolated from ovaries of lagurus lagurus

    首先根據已發表的五種嚙齒目動物zp3cdna序列同源性分析結果設計特異性引物， rt - pcr擴增草原兔尾鼠zp3 （ lzp3 ） cdna保守序列。
  11. Depending on the degenerate primers which were designed according to the conserved amino acids of glycine betaine secondary transporter, a fragment about 1 kb was obtained by pcr. the pcr fragment was purified and labeled with dig as a probe

    將其總dna用sau3ai部分酶切后，收集4 15kb大小的酶切片段，克隆到用bamhi酶切的puc18中，構建halobacillussp . d8基因組文庫，共獲得約9000個重組質粒。
  12. Halobacillus trueperi accumulates glycine betaine under condition of high osmolarity. a partial fragment of the glycine betaine transporter beth gene was obtained from the genome of h, trueperi with degenerate primers. through southern blot hybridization and inverse pcr, a 5. 1 kb ecori fragment containing the beth gene was sequenced

    將擴增片段用地高辛標記成探針，與用不同限制性內切酶完全酶切的h . trueperi總dna片段作southern雜交，結果顯示在ecori酶切片段的5 . 1kb處有陽性信號。
  13. With h. trueperi genomic dna and degenerate primers, a 560 bp pcr fragment was obtained and labeled as a probe. after h. trueperi genomic dna was digested with different endonucleases, southern blot result showed a 2. 6 kb positive fragment digested by ecori and ipcr was carried out to obtain the flanking sequence

    將擴增片段用地高辛標記成探針，與用不同限制性內切酶完全酶切的h . trueperi總dna片段作southem雜交，結果顯示在ecori酶切片段的2 . 6kb處有陽性信號。
  14. The purpose of this thesis was to clone two different group 3 lea genes. on one hand, we designed and synthesized a pair of degenerate primers according to the foreign reports. a 500bp dna fragment was obtained with pcr technique from the dna of highly drought - resistant china leymus

    一方面，參照國外報道幾種植物的第三組lea中的hva1基因的相對保守的氨基酸序列，設計併合成一對兼并引物，提取抗旱、耐鹽性強的羊草的dna ，採用pcr技術，擴增出了495bp的一段基因片段。
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