design promoter 中文意思是什麼
design promoter
解釋
設計倡議人-
7. at the first time, the reporter dye, fam was linked to the 5 " - end of the oligonucleotides of the probes, and the tamra was located at the 3 " - end as quencher dye. we use camv35s and fmv promoter, nos terminater, mark gene nptii, and aim gene pat, epsps and cryla ( b ) genes as target sequences, design pairs of sp
7 、首次以fam熒光素標記探針5 』端作為發光基團，以tarma標記探針3 』端為淬滅基團，以camv35s 、 fmv啟動子、 nos終止子、標記基因nptll 、抗除草劑基因epsps 、 pat 、抗蟲基因cry1a ）為檢狽目標，設計、篩選出特異性引物和探針，優化實驗參數，建立了轉基因植物通用性熒光pcr定性檢測方法體系。 -
In abroad, the study of integration site used for transgenic detection had just begun. in this study, according to the collection of the global commercialized transgenic crops, select seven exogenous genes which basically cover the total commercialized crops, namely camv35s and fmv promoter, nos terminater, mark gene nptii, and aim genes pat, epsps and cryia ( b ). use endogenous 18srrna gene as collate, design a large pairs of specific primers, screen the optimum primers groups, optimized the test condition and parameters, establishing the qualitative pcr detection system
本研究根據收集的國內外已商品化的轉基因作物品種，選擇了能基本覆蓋商品化轉基因品種的7個外源基因，即： camv35s 、 fmv啟動子、 nos終止子、 npt標記基因和目的基因pat 、 epsps 、 cryia （ b ）作為篩選目標，以植物18srrna基因作為內源參照基因，設計了多對特異性引物，並篩選出最佳組合，優化了檢測條件和參數，建立了pcr定性檢測方法體系。
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