dna cleavage 中文意思是什麼
dna cleavage
解釋
dna裂解-
In this sense, ida plays dual effects, tridentate chelating to cu ( 2 ) and bridge between two cu ( 1 ) andcu ( 2 ). 3 metal complexes were selected as the appropriate for the study of cleavage plasmid pbr322dna by gel electrophoresis technique. the results showed ni and mn complexes could cleave effect - ively dna in the presence of h2o2 at physiological ph and temperature, whereas individual zn complex could cleave effectively dna
通過電泳實驗研究了一系列金屬配合物與pbr322dna的作用，發現在tris - hcl緩沖溶液中，生理條件下，鎳、錳配合物在共反應物h _ 2o _ 2存在下能夠很好的斷裂dna ，而zn配合物單獨作用就能夠使dna由ccc型轉化為oc和linear型。 -
With the rapid development of molecular biology, the researchers of different scientific background are provided with a good opportunity to enter the field. people can resolve some important difficult problems with all kinds of research methods and knowledge in their fields. it is chemists " tribute that they design and synthesize effective nucleic acid cleavage reagents and clarify the reaction mechanism of complexes and dna, which makes it possible to search effective remedial reagents and structural probes by molecular design
分子生物學的迅猛發展為不同科學背景的研究者涉足該領域提供了良好的機遇，人們可以利用各自領域的研究方法和知識來攻克生物學中的一些重要難題，化學家所能做的貢獻就是設計和合成一些特異識別和高效切割的核酸斷裂試劑，並闡明其作用機理，從而使通過分子設計尋找有效的治療試劑和結構探針成為可能。 -
The results showed mn and ni complexes possibly bind to dna by the mode of interaction, whereas zn complex possibly bind to dna by the modes of interaction and electrostatic binding. 5. in addition, we conjugated cleavage system with recognize system and analyzed joint products by hplc, which provide experimental basic for design of dual effects cleavage
此外，本文還選用咖啡酸純品來突破切割體系與識別體系（用氨基臂修飾的寡聚脫氧核苷酸）的連接，並用高效液相色譜法分析其偶聯產物，為今後設計併合成一種具有特異識別和高效切割雙重功能的人工核酸酶提供了實驗基礎。 -
In the second part, the influences of la on micronucleus rate were observed by using the rat marrow cell micronucleus test. and the cleavage action of la on genome dna were studied too. the results manifest that a certain concentration of la can increase micronucleus rate obviously and induce the cleavage action and structural change of genome dna
（二）採用小鼠骨髓細胞微核檢測技術研究了稀土元素鑭對微核率的影響，同時採用體外培育技術和紫外分光光度法研究了鑭對基因組dna的斷裂作用，結果表明一定濃度的鑭能引起微核率顯著升高，並可導致基因組dna的斷裂以及結構的改變。 -
A dna fragment of 348 - bp amplified from the b subunit gene was cut into two dna fragment of 216 and 132 - bp by haelli. endonuclease restriction analysis of the plasmid content with psti showed that strainas with the same result of southern - blot with spesific probe had the different cleavage pattern. the isolated 285 - bpand 348 - bp dna was ligated with plasmid puc18. the ligation mixture was used to transform e. coli jm109and the transformants were plated on lb agar containing antibiotics. plasmid dna containing cloned genes were used for direct sequencing
提示1999年的疫情由不同的病原菌引起。另外使用針對志賀毒素2及其變種的引物對進行pcr檢測、細菌染色體pst和pcr產物的hae 、 ras酶切分析，以及pcr產物的序列分析，發現2000年從江蘇省徐州市患者和家畜家禽糞便標本分離的大腸桿菌o157 ： h7菌株僅僅攜帶slt2vha基因。 -
In this study, the suitable parameters for the introduction of plasmids phz1358 and pset152 into s. nanchangensis ns3226 from e. coli were tested for developing an conjugation system for s. nanchangensis ns3226. a dnd gene cluster, which encodes an unknown modification system for 5 hvidans 1326 and renders its dna susceptible to site - specific double - strand dna cleavage during electrophoresis was conjugated from e. coli into s. nanchangensis ns3226
將克隆在整合型載體pset152上的變鉛青鏈黴菌1326的dnd基因簇通過接合轉移導入野生型南昌鏈黴菌ns3226中進行異源表達，觀察到接合轉移子的dna獲得了在含fe ~ （ 2 + ）的電泳緩沖液中電泳時降解的表型。 -
The detection of ribozyme gene with two cleavage sites that cleaves plrv replicase gene were present in the second part of this paper. a conserved sequence of 35s promoter of plrv was found in gene pools. two primers were designed based on the conserved sequence and bamh i and ribozyme gene. the genomic dna of potato was amplified by the primers through polymerase chain reaction ( pcr )
從許多資料中報道的plrv的35s啟動子的序列中找出一段保守序列，然後根據與核酶緊密相連的bamh和這段保守序列設計兩段引物，用這兩段引物通過pcr擴增轉基因馬鈴薯的基因組dna ，並進行檢測。 -
This unusual modification causes wild type s. lividans 1326 dna sensitive to site - specific oxidative double - strand cleavage ( dnd phenotype ). preliminary study revealed that such dna modification involves incorporation of sulphur. the entire functional dnd cluster, 8. 3kb in size, including 3 orfs - dnda, dndb and dndc was involved in this dna modification
野生型變鉛青鏈黴菌具有一種不同於甲基化修飾的新型dna硫修飾系統，使dna在電泳時易遭到氧化雙鏈切割而導致降解（ dnd ， dnadegradation ）表型（ zhouetal . ， 1988 ， 1994 ， 1999 ） 。 -
Constructed the fusion protein expression vector of echistatin, . and identify it by dna sequencing. the vector can express echistatin fusion protein at a high level, the fusion protein molecular weight is about 16000 da in sds - page analysis, and the fusion protein consists more than 30 % of total protein, the fusion protein has specific antigen - antibody reaction with rabbit anti - echistatin polyclonal antibody. the fusion protein include : hpk5. his + 6tag, and echistatin. echistatin can be released by cnbr cleavage
構建了echistatin融合蛋白表達載體，經dna測序鑒定正確后，溫控誘導表達，獲得了高效表達，表達產物經505一隊ge分析，分子量為16000oa ，符合預計的融合蛋白分子量，表達產物. ll 』菌體總蛋白的30 %以上， westem一blotting結果顯示，該日的蛋白能夠和echistatin多克隆抗體發生特異性抗原抗體反應，表明我們成功構建了eohistatin的高效融合表達克隆。 -
In this paper, a series of transition metal complexes were synthesized ; interaction and reaction mechanism between complexes and dna were studied. in addition, we synthesized and analyzed a sequence - specific cleavage reagent, in which oligodeoxynucleotide was recognizing group. detailed work mentioned below were carried out : 1
本文在查閱大量文獻的基礎上，以1 ， 10 -鄰菲咯啉和1 ， 10 -鄰菲咯啉- 5 ， 6二酮為配體合成了一系列過渡金屬配合物，詳細研究了它們與pbr322dna的相互作用及其機理。 -
A reverse - transcriptase polymerase chain reaction ( rt - pcr ) based technique was developed to detect newcastle disease virus ( ndv ) of different poultry species origin. four oligonucleotide primers, based on the differences of nucleotide sequence at the cleavage site of fusion ( f ) protein gene between virulent and non - virulent strains of apmv - 1, were designed to amplify specific dna fragment from different viruses
依據apmv - 1融合蛋白（ f ）基因裂解位點的核苷酸序列與其毒力的相關規律，分別設計合成了四條寡核苷酸引物，建立了一個可迅速檢測不同禽源apmv - 1並可鑒定強、弱毒株的逆轉錄酶?聚合酶鏈式反應（ rt - pcr ）技術。 -
A novel dna modification discovered in streptomyces lividans is different from dna methylation. this unusual modification causes wild type s. lividans dna sensitive to site - specific oxidative double - strand cleavage ( dnd phenotype, dna degradation )
變鉛青鏈黴菌的dna異常修飾系統是一種不同於甲基化修飾的新型修飾系統，它可使變鉛青鏈黴菌dna在電泳時易遭到氧化雙鏈切割（ dnd表型， dnadegradation ） （ zhouetal . ， 1988 ） 。
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