dna library 中文意思是什麼

dna library 解釋
脫氧核糖核酸庫
  • dna : 1. deoxyribonucleic acid 【生物化學】去[脫]氧核糖核酸。2. deoxypentose-nucleic acid 去[脫]氧戊糖核酸。
  • library : n. 1. 圖書館，圖庫；藏書樓；藏書。2. 叢書，文庫。
  1. Even so, by truncating hbv pres gene, we finally obtained some useful " " bailors ", either nontoxic or self - activating, and used them to fish dna fragments of hbv pres interacting protein ( s ) from an ad vector constructed human embryonic cdna library

    我們通過第回軍巨大學碩士學位論文對pres基因分段截短的方法，獲得了對酵母細胞即無毒性作用，又沒有自激活作用的「誘餌」 ，通過它在酵母雙雜交系統中篩選構建於ad載體的人胎肝。
  2. These are replicated individually in bacterial cells to produce a library of different dna clones.

    它們各自在細菌細胞內復制，產生不同的DNA克隆的「文庫」。
  3. Then cdnas and puc18 vectors were linked by t4 dna ligase and transformed into e. coli strain dh5 - alpha to generate cdna library that size is 4. 9 l06 recombinants

    將cdna與載體連接，並導入dh5感受態細胞中，構建成cdna文庫。
  4. Culture of mg7 hybridoma cells and detection of antigen - binding affinity of mg7 mab by elisa 2. construction and identification of mg7 recombinant phage antibody library mrna was isolated from cultured mg7 hybridoma cells and converted into cdna ; the variable fragments of heavy and light chain were separately amplified and assembled into scfvs with a specially constructed dna linker by pcr. the scfvs dma was ligated into the phagmid vector pcantabse and the ligated sample was transfered into competent e. co / / tg1 to generate a bacterial form of mg7recombinant phage antibody library ; volume and recombinant ratio of the library were evaluated by means of bacterial colony counts and restriction analysis ( ecor i and hind iii )

    Mg _ 7重組噬菌體抗體庫的構建及鑒定從培養的mg _ 7雜交瘤細胞中提取並分離mrna ，反轉錄成cdna ；利用pcr分別擴增mg _ 7單抗的重鏈及輕鏈可變區基因，並通過? dna連接子將二者連接起來形成mg _ 7單鏈抗體基因；將mg _ 7單鏈抗體基因插入pcantab5e ；將連接產物轉化感受態tg1大腸桿菌，制備細菌形式的mg _ 7重組噬菌體抗體庫；通過菌落計數和限制性酶切分析（ ecor和hind ）評估mg _ 7重組噬菌體抗體庫的容量和重組率。
  5. To search proteins that associate with the mouse mint protein and regulate notch signaling in nuclei, and to study the function and mechanism of mint - mediated transcription repression, yeast two - hybrid assay was used to screen proteins that interact with a fragment of mint ( f5, amino acids 2226 - 2959 ). from 4x106 yeast clones transformed with the bait plasmid and a cdna library of 9 dpc mouse embryo, fifty - one were positive for nutritional screening and p - galactosidase assay. restriction digestion identified 10 independent positive clones and these were analyzed by dna sequencing these clones represent 3 correctly fused cdna fragments, which are mint, alpha a - crystallin - binding protein i ( alphaa - crybpl ), and nuclear receptor co - repressor 1 ( n - corl ), respectively

    鑒于mwt基因編碼區較長，共有10799個堿基，故此我們將mint分為六段，分別命名為fi一f6 ，本研究以其中的f5 （ 222e959 ）和f6 （ 296s576 ）片段為研究對象，將h者分別插入pgb盯7載體中，結果顯示： mintfs可與核受體輔助抑制因子1階cori ） ， o晶狀體蛋白結合蛋白1hlphatcrybp入及mw加互作用，而f6可與igm的重鏈恆定區、泛素結合酶2l6和一個未知功能的新的蛋白基因進行結合。
  6. The 7mer peptide and 12 mer peptide selected from the peptide library elicited strong anti - vegf antibody immuno - reaction in mice. dna sequenseing showed that gwyydal and vasavfysalve mimic the discontinuous epitope of vegf by the 14 - 17, 21 and 21 25 amino acid residues respectively

    兩者可能分別模擬了vegf分子的21 、 25位氨基酸和14 、 17 、 21位氨基酸通過折疊后在三級結構水平上形成的結合位點和vegf競爭結合受體。
  7. The master geneticists then designed various species, some human, some animal, by playing with the varieties of dna that the sentient civilizations contributed to make earth into this exchange center of information, this light center, this living library

    於是大師基因設計各種族類，一些人，一些動物，通過播放意識文明捐獻給地球的多種多樣的dna進入信息交換中心，這光中心，這生命圖書館。
  8. In the process of library construction, some technical details were studied, such as the combination of dna silver staining with rd technique, purification of pcr product, storage of the data about probes and bioinformatics analysis of one est, etc. 2

    在文庫構建過程中，對其中的一些技術細節，如在dna銀染與rd技術結合、 pcr產物純化、探針數據的管理、 est的生物信息學分析等方面進行了探索。
  9. Constructing cdna expressing library of erythrocytic plasmodium falciparum from hainan : the total rna was obtained by using. triplix kit. a modified oligo ( dt ) primer ( cds ffi pcr primer ) was used in the single - stranded ( ss ) dna synthesis reaction. the ss - dna was reversely transcripted from total rna. double - stranded ( ds ) cdna was amplified by long - distance ( ld ) pcrafter the digestion with proteinase k and sfi i, the cdna with no less than 200bp was collected and purified by glass - milk kitthe library was constructed after the ligation of cdna to tiplex2 phage particle packaged with the packaging extract system in vitro. a high titer and high recombinant ratio of cdna library was constructed

    構建惡性瘧原蟲海南株紅內期cdna表達文庫提取紅內期惡性瘧原蟲海南株總rna ，直接以總rna為模板使用cdna文庫構建試劑盒，首先反轉錄合成ss一dna ，再擴增合成ds一dna （ cdna ） ，對擴增產物用蛋白酶k消化及左z丁i酶切，抽提蛋白、去除rna后，用玻璃奶試劑盒純化、回收20obp以上的片斷，經與載體連接再用蛋白包裝物包裝后形成未擴增文庫，最後擴增完成惡性瘧原蟲海南株紅內期cdna表達文庫的構建。
  10. These are replicated individually in bacterial cells to produce a library of different dna clones

    它們各自在細菌細胞內復制，產生不同的dna克隆的「文庫」 。
  11. The gpa1 gene was obtained via pcr amplification and was cloned in the two - hybrid dna binding domain vector pgbkt7 the combinant plasmid was designated as pgbkt7 - ga. - galactosidase assay indicated that got did not have the property of self - activation. after pgbkt7 - ga was transformed to yeast pj69 - 4a, we transformed arabidopsis vegetative tissue two - hybrid cdna library plasmids to yeast pj69 - 4a containing pgbkt7 - ga

    通過pcr擴增得到gpa1基因並將其克隆到雙雜交dna結合域載體pgbkt7中，得到的重組質粒命名為pgbkt7 - g ， ?半乳糖苷酶活性鑒定表明g不具有自激活特性，將其轉化到酵母菌pj69 - 4a中，再以此為受體菌轉化擬南芥綠色營養組織cdna文庫質粒。
  12. In this paper, we constructed the genomic dna library of nephila clavipes with the vector supercos 1 cosmid

    以dig - oligo2為探針，菌落原位雜交篩選cosmid文庫，得到56個陽性重組子。
  13. Correct clones were selected and plasmid dna was isolated and digested with saci and puvii. a dna fragment of about 2. 1kb was purified and labeled by dig - 11dutp as probe. at least 40 positive clones were obtained from human genomic library by in situ colony hybridyzation with this probe. among them one clone contains human serum album dna by sequence

    以pcr擴增的人血清白蛋白（ hsa ）基因片段為探針，從人的基因組文庫中雜交篩選的陽性克隆中，經測序分析，有一個克隆含有全長hsadna ，用從其它的陽性克隆中選取兩種dna片段，即dna修復基因hfen1和一段非編碼大片段cit987sk - 384d8 ，與人hsadna一起，進行顯微共注射，成功制備了轉多基因小鼠。
  14. After pcr checking and electro - transformation plasmid from c. elegans into dh5a, isolating plasmid from dhsct, digesting them with restriction enzymes and dna sequencing, six cdna fragments, which protein products can interact with rapgap, from cdna library were got

    將pcr擴增陽性及或lacz強陽性質粒電轉化入dhsa細菌，提取質粒后酶切、測序。發現有6個來源於celegqnscdna文庫的dna片段編碼的蛋白可以與ra帥ap相互作用，其中兩個為rapgap 。
  15. A fusion - trascriptional library was then constructed by inserting sau3ai - partially digested chromosomal dna from 7653r into bamhi cloning site of phn127. a group of constitutive - expression fusions with different fluorescent strength were obtained

    將7653r的總dna經sau3ai部分酶解並克隆到phn127上，獲得的融合子庫，從中篩選到gfp組成型表達的具有調控活性的dna片段。
  16. In 1989 we created a library of h. pylori genes by inserting selected fragments of the bacterium ' s dna into cells of e. coli

    我們在1989年時，將選取的幽門螺旋菌基因片段插入大腸桿菌的dna中，讓大腸桿菌製造幽門螺旋菌的蛋白質，以此建立了幽門螺旋菌的基因庫。
  17. It is used as the bait to screen azospirillum brasilense sp7 genomic plasmid library which was constructed by fusing 0. 5 - 3kb fragments of a. brasilense sp7 genomic dna to the dna - activation domain in the pgad plasmid vectors

    Brasilensesp7的nifa全序列構建在pgbd - c2載體上，得到重組質粒pgbd - nifa ，以其為誘餌，篩選sp7質粒基因組文庫（構建在pgad系列質粒上） 。
  18. Its genomic dna was partially digested by sauial. dna fragments from 4 to 16 kb were collected after electrophoresis and ligated with bamhi - digested puc18 to construct genomic library. the total number of recombinant plasmids is about 9000

    進一步在opua基因的上下游序列分別設計引物，從h . trueperi基因組dna中擴增出所預期大小的片段，測序驗證已獲得opua基因的全序列。
  19. In the present paper the application of dna library, phage display random peptide library, phage antibody library, etc. in the research of serodiagnostic reagents and their special values were reviewed

    本文綜述了基因文庫、噬菌體展示肽庫及噬菌體抗體庫技術在血清學診斷試劑研製中的應用及其各自的優點。
  20. A genomic dna library of atriplex centralasiatica iljin was constructed using lambda embl3 / bamh i as vector

    為了得到badh基因的上游序列，我們以lambdaembl3 budl為載體，構建了鹽生植物中亞濱茵的基因組文庫。
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