domain of a protein 中文意思是什麼

domain of a protein 解釋
（蛋白質結構域）：氨基酸序列的一個整體連續的部分，具有某種具體的功能。

  • domain : n 1 領土，版圖；領地。2 管區，勢力圈；（特定動物等的）生長圈；（學問、活動等的）領域，范圍；【物...
  • of : OF ＝Old French 古法語。
  • a : an 用在以母音音素開始的詞前〉 indefinite art 1 〈普通可數名詞第一次提到時，冠以不定冠詞主要表示類...
  • protein : n. 【化學】朊，蛋白（質）。
  1. The dna sequence had a complete orf ( open reading frame ), which coded a protein of 479 amino acid residues. the protein sequence of enolase which contained the conserved domain, was homologue to enolase of other organisms. it showed 83 % ideaity and 89 % similarity compared to the enolase in chlamydomonas reinhardtii

    並將其中的一個gus基因用目的片段烯醇酶基因替換，構建了可以在植物中高效表達的載體pcambia2301g一enolase ，成功地將其轉入根癌農桿菌eha105中，為下一步進行轉基因植物的研究作準備。
  2. Nogo - 66 receptor, ngr, cloned in 2001, is a leucine - rich - repeat glycophosphatidylinositol - anchored membrane protein which mediates nogo - 66 inhibition of axonal outgrowth. both the long acidic amino - terminal domain and the nogo - 66 fragment have strong neurite growth inhibitory activity suggest that nogo - a has at least two inhibitory domains. northern blot, in situ hybridization, western blot and immunocytochemistry analyses show that in addition to oligodendrocytes, nogo - a mrna and nogo - a protein are also expressed in neurons in developing and adult brain and spinal cord, nogo - a is also found in peripheral organs such as heart and testis

    Northernblot 、原位雜交、 westernblot和免疫組化結果證明： nogo amrna和nogo蛋白除了在cns的寡突膠質細胞中表達，還表達于發育階段和成年的腦、脊髓和外周神經節的某些神經元中，在外周組織如睪丸和心臟也有表達； nogoe在cns和pns以及多種外周組織中有廣泛分佈； nogo （除表達于腦和心臟外，在骨骼肌中有較高表達。
  3. The three isoforms have a common carboxy - terminal domain of 188 amino acids, and this region is highly homologous to the reticulon protein family

    在nogo分子的c -末端，有兩個跨膜片段，被66個氨基酸殘基組成的環狀結構（ nogo - 66 ）所分隔。
  4. The extracellular 100pl ( ecl1 ) and ecl2 was linked by disulfide bond to maintenanced the stability of the protein secondary structure. in recent years, we showed that ccr5 as a co - receptor could interact with hiv - 1 infect ion. ccr5 was paid closed attention to since it was cloned in 1996. the aim is to - obtain the sequence of first extracillular domain of 3 chemokine receptor 5 ( ccr5 ) n - terminal gene fragment with high level expression in e. coli and to prepare its specific antibody f ( ab ' ) ;, and its detected method

    Ccr5具有g蛋白偶聯受體家族（ g - proteincoupledreceptors ， gpcrs ）所特有的7個跨膜區（ transmembrinedomains ， tm ） ，呈螺旋， tm的氨基酸有很高的保守性，膜外第一襻（ extracellularloop1 ， ecl1 ）和ecl2之間有二硫鍵相連，以維持蛋白質二級結構的穩定性。
  5. To get in vivo evidences that apoplast calmodulin con 1d regulate plant growth and development process, a chimeric secretion form of calmodulin binding peptide, which contains a signal peptide, a calmodulin binding domain and a c - myc epitope was constructed. the chimeric gene was introduced into arabidopsis. it was expected that the overexpression of this chimeric protein could be secreted into cell wall and bound to apoplast calmodulin, which could reduce the apoplast calmoduin concentration to make an apoplast camodulin " antisense " plant. by observing the potential phenotype change of apoplast calmodulin " antisense " plant, the in vivo function of apoplast calmodulin on plant growth and developmental process could be speculated

    但這些多是採用生理學手段和藥理學方法而得出的體外（ invitro ）實驗結果，為了取得質外體cam在植物生長發育過程中發揮重要作用的invivo實驗證據，根據動物中的一些研究方法，本實驗設計並構建了帶有信號肽、 cam結合肽（ can小肽） 、 epitope （ c - myc ）融合基因的載體，並將融合基因通過真空滲入法轉入擬南芥，預期過表達的融合蛋白將會被分泌到細胞外並與質外體cam相結合，這樣就會抑制質外體cam的功能，從而可以構建質外體cam的「反義」植株，通過觀察質外體cam 「反義植株」的表型改變，就可以推斷質外體cam在植物生長發育過程中的功能。
  6. The cyst cells enclosing spermatomeres maybe synthesize a kind of scf - like protein, which can recognize specially the c - kit receptor on the cellular membrane of spermatomeres. then c - kit is activated, dimerizing and autophosphorylating. at the same time, the tyrosine kinase domain of c - kit is activized, which phosphorylates the proteins that have sh2 domain

    精母細胞周圍的囊細胞可能合成scf樣蛋白，特異地識別精母細胞膜上的c - kit受體，並刺激c - kit發生二聚化、自體磷酸化，激活胞內酪氨酸激酶活性，活化具有「 sh _ 2結構域」的靶蛋白，可能通過一系列信號級聯，最終激活與減數分裂的相關蛋白或基因。
  7. An unusual rice calmodulin isoform, oscam61, was first obtained in our lab, which contains an n - terminal cam domain and a c - terminal basic extension with a potential prenylation site. in vitro activity assays confirm oscam61 as a functional calmodulin. using the green fluorescent protein ( gfp ) as a visual marker, we further studied subcellular localization of oscam61 in stably transformed tobacco cells

    利用綠色熒光蛋白（ greenfluorescentprotein ， gfp ）作為標記，研究了oscam61在煙草細胞中的定位， gfp - oscam61融合蛋白（具有開放的異戊烯化修飾位點）定位於細胞質膜和細胞器膜上，而oscam61 - gfp （異戊烯化修飾位點被gfp封閉）定位於細胞核的核質中。
  8. The predicted vfcpkl protein with 493 amino acids contains all the structural characteristics of reported cdpks and there are four domains of a variable domain, a kinase domain, an autoinhibitory domain and a calmodulin - like domain from n - to c - terminal end

    由編碼區推測的vfcpk1的493個氨基酸序列具有已報道的cdpk的所有典型結構特徵，由n端到c端可分為可變區、激酶區、連接區和調節區四個結構域。
  9. The tn5 inserted in the orf4. it was demonstrated that this fragment was related to magnetosome biosynthesis by experiment of functional complement. the orf4 encodes a protein which is homologous to the atpase from magnetospirillum magnetotacticum ms - 1 and contains a rec domain which has a chey - homologous receiver domain

    該片段由6個orf組成， tn5插入在3621bp - 3622bp間，位於orf4起始密碼子下游的84bp處， orf4因tn5插入失活，通過功能互補實驗證明該片段與磁小體合成相關。
  10. By using western - blot, the fusion protein could not be react with antiserum to prrsv, whereas it presented a reactivity with swine antisera against prrsv and mab ge3 by elisa the results implied that the epitope might be one conformational epitope that was mainly composed of aa50 ~ aa55 domain on n protein

    Western - blot分析表明表達產物與prrsv陽性血清沒有反應性，而elisa分析表明表達產物可與prrsv陽性血清和單克隆抗體發生反應。由此說明單克隆抗體ge3所識別的抗原表位可能是存在於n蛋白上以kphf為核心的構象型表位。
  11. The comparative analysis of sequences indicated that the sequences of meq in different pathotypes are relatively conserved and the homology of the amino acid sequences is very high. the significant differences include two mutations in both mdv - 1 vaccine strains cvi988 / rispens and 814 strain : the deletion of a proline ( no. l93aa ), and this mutation is just exactly located in a 15 - amino - acid ( eelcaqlcstppppi ) repeat sequence within the c - terminal transactivation domain of meq protein ; and a point mutation with a shift from alanine ( a ) of all virulent strains to serine ( s ) was occurred on the no. 71 aa

    結果發現， mdv不同致病型的meq基因序列相對比較保守，它們相互間核苷酸和氨基酸序列的同源性均很高；但是，與所有七個致瘤性的mdv毒株相比，二個型弱毒疫苗cv1988 rispens株和814株均出現了兩個特徵性的突變：即第194位的p缺失性突變和第71位氨基酸由a變成了s的位點突變；缺失性突變恰恰位於meq基因中轉錄激活域內的一個多脯氨酸的重復序列（ pppp ）之中。
  12. At the n - terminal end is the variable domain of 26 amino acids in length, which has no conserved mgxxc ( s / q ) xxt sequence essential for n - myristoylation. so it is conferred that the vfcpkl is a soluble protein

    N端的可變區有26個氨基酸殘基，沒有豆蔻酰化所必需的保守序列mgxxc （ s q ） xxt ，因此推測vfcpk1可能是水溶性蛋白。
  13. Hydrophobicity analysis predicted a 36 - residue hydrophobic signal peptide for secretion in the n - terminus, and no transmembrane region was present, suggesting it might be a type of secretory protein. some generic and atipical n - glycosylation sites were present in clsp, suggesting that the protein might represent a glycoprotein. the clsp protein contained one cub ( clr / cls, an embryonic sea urchin protein uegf, and bone morphogenetic protein i ) domain from 39th to 164th ammo acids, which is known to be involved in protein - protein or protein - substrate interaction, and a trypsin - like serine protease domain positioned from 244th to 483rd amino acids

    Clsp分子具有補體樣絲氨酸蛋白酶的多種結構特徵，包括36個氨基酸組成的疏水信號膚，一個cub （ clr / cls ， anembryonicseaurehinproteinuegf ， andbonemorphogeneticproteinl ）結構域和一個胰酶樣絲氨酸蛋白酶結構域（ t汀psin一likeserineproteasedomain ）和幾個保守的糖基化位點等，沒有發現有跨膜區的存在。
  14. Huctla4 - lg, " one of the most potent in1n1unosuppressive molecules, is a soluble recombinant fusion protein ( molecu1ar weight of approximately 92 kd ) consisting of the extracellular domain of human ctla - 4 and a fragment ( hinge and constant region ) of the fc portion of human iggl. it strongly adheres to the b7 molecule to block the cd28 - mediated costimulatory signal, resulting in inhibition of in vitro and in vivo immune response

    可溶性重組融合蛋白huctla4 - ig是一種新穎的免疫抑制劑，分子量約為92kd ，由人ctla - 4分子的胞膜外區與人igg1的fc段（鉸鏈區與恆定區）融合形成，能牢固地與b7分子結合，阻斷cd28介導的共刺激信號，導致免疫反應的抑制。
  15. Its gene includes seven exons ( 3 - 9 ) and six introns ( 3 - 8 ). with respect to zfghrl, it is also like other ghr1s, which consists of 1578bp and encode a 528 amino acid protein. its gene only includes eight exons ( 1 - 5, 8 ) and seven introns ( l - 5 ). various methods used to find out the transmembrane and boxl domain of zfghrl but the result turns out to be fruitless due to unknown reason. fghr2 and zfghr2 : fghr2 includes eight exons and seven introns, which consists of 1710 bp and encodes a 569 amino acid protein ; zfghr2 includes eight exons ( 1 - 5, 8 ) and seven introns ( 1 - 5 ), which consists of 1758 bp and encodes a 484 amino acid protein

    得到的fghr1含有7個外顯子（ 3 - 9 ）和6個內含子（ 3 - 8 ） ，由1755個bp的核苷酸組成並編碼一個584個氨基酸殘基的蛋白質：得到的zfghr1包含有1587個核苷酸並編碼一個528個氨基酸殘基的蛋白質，理學碩士學位論文:南方貼生長激素受體cdna的分子克隆和魚類存在兩種生長激素受體的證明其基因只有8個外顯子（ l一5 ， 8 ）和7個內含子（ 1一5 ） 。
  16. It encodes a protein of 865 amino acids with a signal peptide at the n - terminus, of which a polycystic - kidney - disease ( pkd ) - like domain, a triosephosphate - isomerase ( tim ) catalytic region, a receptor - for - egg jelly ( rej ) - like domain and two tandem chitin - binding - domains ( chbds ) locate from the n - to c - terminus

    根據已知幾丁質酶基因的dna保守序列，利用人工合成的pcr引物和cb101總dna ，擴增出一個約2 . 6kb的片段並克隆到pbluescript ks載體上。
  17. The secondary structure of this protein speculated had a typically conserved domain of cytochrome p450 cysteine heme - iron ligand signature ( fnagprlcig ) and a hydrophobic domain at n - terminal of amino acid

    （ 6 ） c鉀86mf同源基因片段在13個物種中的序列分析表明:它們的核昔酸差異為1
  18. It shows that the longest band ( 675bp ) has lower homology with flower - timing genes, but there is 80 percentage of homology with pectinesterase during citrus development, in 184 score of hsp. it is clear to see a functional domain by modeling three - dimension structure of the protein

    結果發現分子量最大的片段（長675bp ）與成花計時基因同源性較低，而與植物發育過程中的果膠酶基因具有80同源性，而且局部片段對比分值達184 ；通過蛋白質三維結構預測發現，它具有明顯的功能結構域。
  19. Blast in genbank indicated that it is novel gene named par - 1. the characteristics of the protein coded by this gene were analyzed by bioinformatics. the result showed that this protein was instable, p1 9. 65, located in bacterial inner membrane, and composed of alpha helix, extrended strand and random coil. there was a srong transmembrance domain and contained some conserved sites such as protein dinase c phosphorylation

    通過網際網路數據庫及生物信息學分析工具進行初步分析表明：該基因編碼的蛋白是一種等電點為9 . 65的不穩定蛋白，定位於細胞內膜；由螺旋、延伸帶、和隨機捲曲三種形式組成，具有蛋白激酶c磷酸化位點等多個位點，並具有一個強跨膜疏水區。
  20. Lfy and lfy - like proteins from angiosperm contain a proline - rich region near the amino terminal and an acidic central region which is typical for transcriptional activators and may be important for the function of lfylike protein. the two domain exists in the variable regions of all lfy - like proteins

    與擬南芥leaf 』 y基因相比較，銀杏ginlfy和g毗基因沒有做為轉錄激活區域的位於氨基酸序列中間的酸性區和位於n端的富含脯氨酸區，但這兩個區域均位於leafy基因的非保守區。
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