double splicing 中文意思是什麼
double splicing
解釋
雙重剪接-
Long - term continuous operation by introducing stored top tape double tape feeder, motorized tape feeder, and tape splicing tool
通過採用雙卡式料架，電子馬達式料架，編帶連接治具，實現長時間不間斷連續生產。 -
This thesis contrasts and analyses the different calculation - method, and improves the genetic algorithm in the parameter inversion of the rock and soil engineering. the strategies are mainly in the following three aspects : 1, new searching way is compositely searching genetic algorithm which is made up of the acceleration method and genetic algorithm when it partially calculates in the later time ; 2, index function is inducted in gengetic operators, at the same time repeated select and double exchange pool are used ; 3, splicing crossover, which reserves some new evolution factions, improves authority function accaunting the capabilities and kinds
本文對優化演算法中不同求解方法進行對比分析，針對遺傳演算法在巖土工程參數反演運算中進行了改進，改進的策略主要集中於三個方面： 1 、提出了在遺傳演算法中融入形加速法的改進方法? ?復合遺傳搜索法； 2 、針對遺傳運算元的選擇策略，引入指數適值方法，設置雙交換池，提出了重復篩選法； 3 、加強對劣勢種群的內部優良信息的遺傳功能的改進，引入權函數，擴大可交叉運算元的種類，保留各運算元的進化功能，提出了融合交叉運演算法。 -
The mechanism is that the introduced complementary oligonucleotides can bind to the corresponding mrna or double - stranded dna in genome and form partial double - stranded molecules or triple - stranded nucleic acid molecules by sequence - specific and nonsequence - specific antisense action, thus the target gene will be orientationally blocked and expression of the target inhibited so that therapeutic effect could be attained. in this study, we designed a fragment of human c ii ta cdna in antisense orientation using mrna of c ii ta as template. the primers were designed based on 94 - 500 nucleotides segment in 5 " end of ciita gene so that the interested gene contained 407 base pairs which included two aug codons in 1 16 and 188 nucleotides as well as the splicing site between the first and the second exons
本研究設計以c tamrna為模板的反義cdna片段，從c ta基因5 』端第94位到500位核苷酸段設計引物，目的片段407bp ，覆蓋第116和188位兩個aug密碼子，也包含了第一外顯子和第二外顯子間的剪接位點：用常規分子生物學方法構建了反義片段的腺病毒表達載體（ padeasy - 1系統） ；腺病毒載體經hek293細胞包裝產生含反義片段的重組腺病毒，用氯化銫密度梯度離心法獲得純化的高滴度腺病毒；進行體外基因轉移，分別用反義片段真核表達載體轉染p388d1細胞和用重組腺病毒感染hela細胞，觀察導入的c ta基因反義rna抑制細胞內組成型或誘導型c ta基因表達的作用，從而達到調控mhc -類分子表達的目的。
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