double-stranded dna 中文意思是什麼
double-stranded dna
解釋
雙鏈dna-
Helicase any of a group of enzymes that catalyze the unwinding of double - stranded nucleic acids such as dna, using energy from atp
解螺旋酶（蝸牛酶） ：一組可以催化象dna這樣雙鏈核酸解螺旋的酶，由atp提供能量。 -
Constructing cdna expressing library of erythrocytic plasmodium falciparum from hainan : the total rna was obtained by using. triplix kit. a modified oligo ( dt ) primer ( cds ffi pcr primer ) was used in the single - stranded ( ss ) dna synthesis reaction. the ss - dna was reversely transcripted from total rna. double - stranded ( ds ) cdna was amplified by long - distance ( ld ) pcrafter the digestion with proteinase k and sfi i, the cdna with no less than 200bp was collected and purified by glass - milk kitthe library was constructed after the ligation of cdna to tiplex2 phage particle packaged with the packaging extract system in vitro. a high titer and high recombinant ratio of cdna library was constructed
構建惡性瘧原蟲海南株紅內期cdna表達文庫提取紅內期惡性瘧原蟲海南株總rna ，直接以總rna為模板使用cdna文庫構建試劑盒，首先反轉錄合成ss一dna ，再擴增合成ds一dna （ cdna ） ，對擴增產物用蛋白酶k消化及左z丁i酶切，抽提蛋白、去除rna后，用玻璃奶試劑盒純化、回收20obp以上的片斷，經與載體連接再用蛋白包裝物包裝后形成未擴增文庫，最後擴增完成惡性瘧原蟲海南株紅內期cdna表達文庫的構建。 -
The cyanophage contained a double - stranded dna genome at ca. 36. 6kb
病毒的遺傳物質為雙鏈dna ，基因組的大小約36 . 6kb 。 -
Much excitement is therefore currently surrounding a new type of virus - - actually a bacterial virus, usually called a phage - - which is circular and double - stranded and which therefore has only a very low tendency to intercalate into other dna at random
因此，令人興奮的是：目前正在圍繞著一種新型的病毒?實際上是一種細菌病毒，通常稱為噬菌體?它是環狀雙線的，因此它隨機插入其他dna的傾向性很低。 -
Dna double stranded breaks, dsbs
雙鏈斷裂 -
Baculovirus is a kind of double - stranded circle large dna virus, which has been developed as an environment sound pesticide and a powerful vector of foreign protein expression as well as a potential vector of gene therapy
桿狀病毒是一類雙鏈環狀dna病毒，作為生物防治殺蟲劑及高效外源蛋白表達載體得到廣泛研究。近來有許多學者致力於桿狀病毒作為基因治療載體的研究。 -
When the new owners came in, they worked in their laboratories and created versions of humans with a different dna - the two - stranded, double - helix dna
當新業主前來，他們在實驗室工作，並創造了不同版本的具有雙螺旋dna的人類。 -
The mechanism is that the introduced complementary oligonucleotides can bind to the corresponding mrna or double - stranded dna in genome and form partial double - stranded molecules or triple - stranded nucleic acid molecules by sequence - specific and nonsequence - specific antisense action, thus the target gene will be orientationally blocked and expression of the target inhibited so that therapeutic effect could be attained. in this study, we designed a fragment of human c ii ta cdna in antisense orientation using mrna of c ii ta as template. the primers were designed based on 94 - 500 nucleotides segment in 5 " end of ciita gene so that the interested gene contained 407 base pairs which included two aug codons in 1 16 and 188 nucleotides as well as the splicing site between the first and the second exons
本研究設計以c tamrna為模板的反義cdna片段，從c ta基因5 』端第94位到500位核苷酸段設計引物，目的片段407bp ，覆蓋第116和188位兩個aug密碼子，也包含了第一外顯子和第二外顯子間的剪接位點：用常規分子生物學方法構建了反義片段的腺病毒表達載體（ padeasy - 1系統） ；腺病毒載體經hek293細胞包裝產生含反義片段的重組腺病毒，用氯化銫密度梯度離心法獲得純化的高滴度腺病毒；進行體外基因轉移，分別用反義片段真核表達載體轉染p388d1細胞和用重組腺病毒感染hela細胞，觀察導入的c ta基因反義rna抑制細胞內組成型或誘導型c ta基因表達的作用，從而達到調控mhc -類分子表達的目的。 -
The genetic material of the new virus is arranged in a similar fashion, encoded in circular, double - stranded dna, and the virus ' s five proteins have similarities to the proteins of other polyoma viruses
新病毒的遺傳物質排列方式與多瘤病毒類似，編碼為環狀的雙鏈dna ，新病毒的五個蛋白質與其他多瘤病毒有相似之處。
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