eg cells 中文意思是什麼
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Eg cells of the 2th and 4th passage were akp ( alkaline phosphate ) positive. when cultured on degenerated feeder layers or in suspension, eg c ells formed embryoid bodies ( ebs ) in vitro
當eg細胞脫離飼養層懸浮培養，或在衰老的飼養層上延遲培養時，發現eg細胞或單個存在，或聚集成團，形成類似於早期胚胎的囊狀胚體結構 -
The stem cells derived from pgcs are called embryonic germ cells ( eg cells )
來源於pgcs的幹細胞稱胚胎生殖細胞。 -
In the experiment, in case of the proliferative capability of eg cells, the composition of 0. 25 % trypsin + 0. 04edta was optimal. the growth behavior of icr murine eg cells from pgc was observed
傳代過程中，以0 . 25胰酶與0 . 04 edta聯合作用效果較好，對eg細胞的綜合損傷最小，離散傳代后eg細胞活力比較強， eg克隆出現最多，最高傳至6代。 -
The purpose of this study was to establish es or eg cells lines in the future for further research. the results attained were as follows : 1. derivation of embryonic stem cells from icr mouse preimplantation embryos
本試驗對icr小鼠胚胎多能幹細胞的建系工作做了初步探索，為胚胎多能幹細胞多種屬廣泛建系的研究打下一定基礎，也為小鼠胚胎多能幹細胞的深入研究積累經驗並提供借鑒。 -
2. isolation and cloning of mouse embryonic germ cells of icr species primordial germ cells ( pgc ) were isolation from 8. 5 - 15. 5 dpc ( days post coitum ) embryos. eg cell lines with the characteristic of murine es cell line were established and continuously cultured to 6th passage
Icr小鼠eg細胞的分離克隆研究本試驗以icr品系小鼠胚胎8 . 5 15 . 5dpcpgc為材料，經傳代培養，獲得能連續而穩定傳至6代的，具有胚胎幹細胞諸多特性的eg細胞系。 -
All the result showed that ndv f48e9 strain has its own speciality compared with other five ndv strains, and there were many difference between velogenic strains and lentogenic strains. so the infectious cdna of rnesogenic strains and lentogenic strain was far from enough to understand the replication, pathogenicity of ndv and the interaction between ndv and host cells, and the infectious cdna of velogenic strains ( eg. f48e9 ) was required to explain the relationships between structure and function
本研究成功地獲得了ndvf48e9 t因組的核昔酸序列，並構建了表達ndvf48e9基因組cdna的低拷貝表達載體休f48e9 ，為構建新城疫病毒強毒株f48e9株的感染性cdna奠定了物質基礎，進一步研究ndv的生物學特性、結構與功能的關系；進一步探討影響ndv毒力的因素、以及研製新型疫苗載體提供了可靠保證。 -
This morphology was slightly different from that of mouse eg cells
這與經典的小鼠胚胎生殖細胞的形態有所不同。 -
At primary culture, pgcs co - cultured with their gonadal stromall cells were well grown. when subculture, we used primary chicken embryonic fibroblast ( pcef ), primary mice embryonic fibroblast ( pmef ) and snl cells to make feeder cells. forward research founded that the pcef cells were the most suitable for the growth of putative eg cells when having various cytokines
繼代培養時，經過反復實驗比較雞胚原代成纖維細胞飼養層（ pcef ） 、小鼠原代成纖維細胞飼養層（ pmef ） 、 snl細胞飼養層等三種不同飼養層的作用效果，最終發現以雞胚原代成纖維細胞製作飼養層同時添加各種生長因子最適合雞類eg細胞的生長，但pcef與pmef之間的差異不顯著。 -
Sns has an energy gap of eg 1. 3ev, which is very close to the optimum energy gap 1. 5ev of solar cells, and it has a high absorption coefficient ( > 104cm - 1 ) and a high conversion efficiency of about 25 %. in addition, elements sn and s are abundant and non - toxic in nature
硫化亞錫（ sns ）的禁帶寬度eg 1 . 3ev ，接近太陽能電池的最佳禁帶寬度1 . 5ev ，在理論上其能量轉換效率達到25 % ;其吸收系數? 104cm - 1 ，用作太陽能電池耗材少;其組成元素s和sn在地球上儲量豐富、廉價、無毒，有很好的環境兼容性。 -
To date, eg cells with proven germ - line transmission have been completely established only in the mouse with embryonic stem cells ( es cells )
迄今為止僅在小鼠上獲得pgcs來源的真正意義上的胚胎幹細胞（ escells ） ，即具有參與生殖系傳遞能力的es細胞。 -
The eg cell culture media consisting of dmem medium supplemented with fbs, chicken serum, beta - mercaptoethanol, l - glutamine. hepes, chicken embryonic extract and cytokines etc. after 24 hours culture, the isolated pgcs were selectively attached on the gonadal stromall cells in the plates
加入新鮮的eg細胞培養液（含dmem 、胎牛血清、雞血清、 -巰基乙醇、 l -谷氨酰胺、 hepes 、雞胚浸出液以及細胞因子等成分）培養24小時以後， pgcs開始部分貼附於共培養的生殖原基細胞上。 -
With the increase of passage, the total of colonies decreased, but the number of classical nest - like or island - like eg cell colonies relatively increased. the growth of eg cells was dependent on the feeder layer
傳代培養的eg細胞對飼養層有依賴性， icr品系小鼠pgc細胞和由其傳代后獲得的eg細胞呈明顯的群聚性，以單個存在狀態的較少 -
This study did microinjection to kunming white mouse inbred embryos to test its in vitro developmental ability, then pluripotency of porcine eg cells was tested with production of chimera by blastocyst injection. finally, first chimeric pig in china was delivered, and by chimera analysis, chimerism is present in some tissues
本研究首先利用近交系昆明白小鼠進行胚胎顯微注射觀察發育效果，然後利用所獲五指山豬eg細胞製作嵌合體檢驗eg細胞多能性，最終獲得國內首例胚胎幹細胞（ eg細胞）嵌合體豬，經dna分析確認在多種組織中存在嵌合狀態。 -
Embryonic stem ( es ) cells were derived from the inner cell mass ( icm ) of preimplantation embryos, and embryonic germ ( eg ) cells from primordial germ cells ( pgc ). both es and eg cells were cultured to determine the factors affecting on isolation, cloning and passage of pluripotent stem cells from icr mouse embryos
試驗以icr小鼠胚胎內細胞團（ icm ）和原始生殖細胞（ pgc ）為材料，採用不同培養體系分離克隆胚胎多能幹細胞，探討icr品系小鼠胚胎多能幹細胞分離克隆的基本方法，以及一些因素對其分離、培養、克隆、傳代等效果的影響。 -
Experiment iii dna analysis of porcine embryonic stem cells ( eg ) chimera
試驗三，豬胚胎幹細胞（ eg ）嵌合體的dna分析。 -
Pgc and eg cells derived from pgc characterized by a typical colony appearance with very scarce individual cell
對傳2代、 4代的eg細胞進行akp染色，所得的eg細胞為akp陽性細胞。 -
A, pgcs collected from fetuses were immediately injected into the embryos after isolation ; b, pgcs were cultured on gelatin - coated multidishes for up to 24 h untill injection ; c, eg cells of 0 - 6 passages
將新鮮和培養24h的pgc 、 6代前的eg細胞進行顯微注射。從42頭供體豬獲得564枚胚胎。 419枚胚胎注射pgc eg細胞，與未注射的145枚胚胎搭配混合後分別移植給22頭受體豬。 -
( 2 ) fetuses at d27 were collected from wzsp and white minitype pigs respectively, the results showed that there is no notable difference in eg cells culturing between the two types of pigs ( p > 0. 05 ). they are all suitable materials to establish porcine eg cell lines
（ 2 ）分別收集d27wzsp和微型白豬胎兒，實驗結果證明， wzsp和微型白豬在eg細胞培養方面沒有顯著性差異（ p 0 . 05 ） ，都可以用來作為eg細胞建系的材料。
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