electrophoresis cell 中文意思是什麼

electrophoresis cell 解釋
電泳槽
  • electrophoresis : n. 電泳（法）。
  • cell : n 1 小室，單室；隔間，艙；〈詩〉茅舍；（單個的）蜂窩，蜂房。2 〈詩〉墓穴，墓。3 （大修道院附屬的...
  1. Our previous studies showed existence of apoplast cam in the plant cell and cam had many extracellular functions. so it supposed cam may be one of important extracellular polypeptides and trigger the intracellular signal transduction by binding the receptor. in this study, radiolabelled ligand is used to investigate the binding characteristic of cam and a. thaliana protoplasts. and chemical crosslinking is employed to explore binding proteins in the membrane. at first, ( 35 ) ~ s - cam was produced using ( 35 ) ~ s - labeled amino acid mixture in e. coli. sds - page and autoradiograph indicated high - purified, high - specific radioactivity ( 35 ) ~ s - cam was obtained. electrophoresis of ( 35 ) ~ s - cam is the same as that of unlabeled cam with ca ~ ( 2 + ) or egta ; a quatitive of protoplasts was prepared by enzymolysis

    首先，用~ （ 35 ） s標記的氨基酸混合物喂養工程菌成功地制備了~ （ 35 ） s標記的擬南芥鈣調素（ ~ （ 35 ） s - cam ） ， ~ （ 35 ） s - cam純度高、放射活度高、 ca ~ （ 2 + ）與egta存在時的電泳行為與未標記cam相同，可作為一種高靈敏性的探針用於進行受體學分析實驗；用擬南芥種子誘導愈傷，通過酶解制備了大量原生質體。
  2. Dna damages caused by so2 and lead acetate were studied with the single cell microgel electrophoresis technique ( or comet assay ) in order to confirm the damaging degree of lead ( as an important component of atmosphere particle matter ) on dna from male mice exposed to so2. the migrating distances of dna of brain, lung, spleen and kidney cells of mice increased significantly, compared to the control group under conditions of single and combined poisoning of so2 ( 42mg / m3 ) and lead acetate ( 0. 2 % ), and lead could strengthen dna damage degree by so2 in nuclear dna of brain, kidney, spleen cells. damaging degree of so2 on nuclear dna of lung cell of mice was more severe than that of lead

    為了明確大氣顆粒物中的重要組分? ?鉛在二氧化硫所致dna損傷中的作用程度，利用單細胞凝膠電泳技術（ singlecellgelelectrophoresis ， scge ，或稱彗星實驗， cometassay ）研究了鉛與二氧化硫的聯合污染，結果表明在42mg m ~ 3so _ 2和0 . 2醋酸摘要一abstract鉛單獨及聯合染毒條件下，小鼠腦、肺、腎、脾細胞dna遷移距離均比對照顯著增加;鉛加劇了50 :對腦、腎、脾細胞核dna的損傷程度; 50 :對肺細胞核dna的損傷程度要比鉛的損傷大，小鼠肺細胞核dna遷移距離在50 :和醋酸鉛聯合作用組與醋酸鉛單獨作用組間有極顯著性差異（ p < 0 . 01 ） ，而與502單獨作用組間沒有顯著性差異。
  3. ( 2 ) the mortality of cp cell in 10 were increasing directly related with the time in the low temperature. after 120d some cp cells died in the way of apoptosis. most nucleus of cells were condensed, and chromosome was marginated beside the nucleus inner membrane, cell sizes were reduced, dna ladder showed in dna gel electrophoresis

    （ 2 ） cp細胞系在10低溫下細胞死亡率與時間成正比， 120d的細胞具典型的凋亡現象，即細胞核固縮、染色體邊集在核膜內側；細胞體積變小；瓊脂糖凝膠電泳上顯示特徵性的「梯狀」帶。
  4. The e. coli strain jm109 was transformed with resultant plasmid pgex - 4t - 1 / 6 - 4. 4. the transformation was induced with iptg, then the total protein from cell extract was analyzed by electrophoresis on a 8 % sds - page in order to validate the gst fusion protein, and the fusion protein is about 90kd

    4 .用工ptg誘導含pgex一4t一1 / 6一4的轉化菌，提取初提物中的總蛋白，進行sds一聚丙烯酞胺凝膠電泳，檢測表達的融合蛋白大小越為gokd 。
  5. The approaches used in the study included : observing the microstructure and ultrastructure of the cell line of colossoma brachypomum ( cbt ) and the cell line of carp ( cp ) stressed low temperatures under fluoroscopy and tem ; analysis of dna damage in the cultured cells under temperatures stress by dna gel electrophoresis

    本研究採用的主要實驗方法：通過熒光顯微觀察、電鏡超微結構觀察確定cbt （淡水白鯧臀鰭細胞）和cp （草魚胚胎細胞）在低溫處理后的顯微與超微結構的變化。應用dna電泳分析細胞dna在低溫處理后的斷裂現象。
  6. Effects of estrogen on cisplatin - resistance human ovarian cancer cell line coc1 ddp : analysis by two - dimensional polyacrylamide gel electrophoresis

    順鉑耐藥的蛋白質雙向凝膠電泳圖譜分析
  7. In this paper, the effect of nuclear actin on the process of chromosome construction has been studied by utilizing the precise natural synchrous plasmodium of physarum polycephalum, sds - polyacryl amide gel electrophoresis ( sds - page ), western blotting, the cell - free system and optics microscopy. the major results and conclusions are as follows : 1

    本實驗以多頭絨泡菌原質團為材料，採用同步化培養、細胞核提取、 sds - page 、免疫印跡、非細胞體系構建、光學顯微鏡觀察等方法，研究了有絲分裂前期核內肌動蛋白對染色體構建的影響。
  8. In order to understand the mechanism of mtx further and to investigate the genotoxic target organs, we studied the dna damage and the correlation with dose of mtx by using the alkaline single cell gel electrophoresis ( comet ) assay. liver, spleen, bone marrow, thymus, kidney, testicle, stomach and peripheral lymphocytes of mice were isolated at lh, 3h, 6h, 12h, 24h after 5mg / kg mtx intraperitoneal injection

    為了進一步了解甲氨蝶呤（ mtx ）的作用機制，探測其作用的遺傳毒性靶器官，為應用mtx治療過程中的臨床監測和副作用防治提供理論依據，我們以小鼠為研究對象，用單細胞凝膠電泳技術檢測了mtx腹腔注射染毒1h 、 3h 、 6h 、 12h 、 24h后對肝、脾、骨髓、胸腺、腎、睪丸、胃和外周血淋巴細胞的dna損傷作用及損傷程度與mtx劑量間的關系。
  9. In this study, the profiles of proteins expesssion in different growth phases of rt19 have been obtained using the technique of proteome analysis with two - dimensional polyacrylamide gel electrophoresis ( 2 - d page ). an efficient imagemaster 2d was used to reveal the number of protein spots corresponding from lag phase to stationary phase, varying from 398 to 516, which manifested kinetic state of proteome in cell

    本文採用高解析度的雙向電泳技術，得到不同生長時期的蛋白表達譜，應用高效的imagemaster2d軟體進行圖譜分析，結果顯示： （ 1 ）從延滯期到穩定期的末期，所表達的蛋白數量由398個逐漸增加至516個，顯示了細胞內的蛋白在不同生長期的動態表達水平。
  10. Progress in single - cell analysis by capillary electrophoresis

    單細胞毛細管電泳分析研究進展
  11. The fundamentals and applications of single cell electrophoresis technique ( scge ) are discussed

    摘要介紹單細胞凝膠電泳技術（ scge ）的原理及其在環境污染物檢測中的應用。
  12. Protection characteristics of taurine on oxidative damage of human retinal pigment epithelial cells detected by single cell gel electrophoresis

    牛磺酸對經單細胞凝膠電泳技術檢測人視網膜色素上皮氧化損傷的保護特徵
  13. We applied single cell gel electrophoresis and cell culture technique, which constitute sing cell gel electrophoresis assay system for detecting mutagenicity to detect mutagenesis in vitro induced by animal drug quinocetone and olaquindox. and confirmed optimum lysing - time. vero cells in the period of logarithm - growth time were treated with 9. 1 ~ 273u mol / l h2o2 at 37 3h, then were lysed for lh, 2h, 3h and 4h to find optimum lysing - time

    並基於陽性致突變物h _ 2o _ 2作用於非洲綠猴腎vero細胞引起細胞dna損傷的原理，研究了其關鍵步驟-裂解時間，以9 . 1 273 mol l劑量的h _ 2o _ 2染毒處于對數生長期的vero細胞3h后，收獲細胞用於制備三明治凝膠板，分別裂解1h 、 2h 、 3h和4h並選擇最適裂解時間。
  14. The enzyme was purified from cell extract by ammonium sulfate fractionation ( 30 % - 80 % saturation ) and consecutive column chromatography using deae - cellulose and sephadex g - 100. the sod activity for purified enzyme could reach 4966 iu / mg proteins, and the molecular weight of the sod was about 20 kda which determined by sds - page electrophoresis. when the non - denaturing polyacrylamide gel stained with substrate for sod in the present of 0. 2mmol / l kcn or 0. 5 mmol / l h2o2, the active band was still showed at the same position, which indicated the sod could not be inhibited by the treatments with kcn or h2o2 and it was the mn sod

    通過sds一聚丙烯酸胺凝膠電泳可知該sod酶的分子量約為20kda .在非變性聚丙烯酞胺凝膠（ pagb ）電泳后，在凝膠son顯色反應液中加入0 . 2耐01 / lkcn或0 . 5inmol / lh202 ，凝膠sod顯色反應后在原來的sod酶部位仍然含有sod活性帶，這表明利用kcn和h20 :處理並不能抑制500的活性，該sod屬于mn一sod 。
  15. Then the strain cell is detected to determine weather there is any plasmid in the cell. the detection result analysed by gelose gel electrophoresis shows that the cell harbors a plasmid, and this plasmid is relatively large

    應用質粒檢測的方法，對菌株細胞進行質粒檢測，並對檢測結果進行電泳分析，實驗發現，菌體細胞中存在一個質粒，而且質粒較大。
  16. Study on ratio of moment move and ratio of inertia move as indices of single - cell gel electrophoresis

    矩類遷移比作為單細胞凝膠電泳指標的研究
分享友人
分享到 Line

分享到臉書