enzyme digestion 中文意思是什麼
enzyme digestion
解釋
酶解作用-
Two primers, designed according to the conserved regions of ban gene in arabidopsis thaliana, were used to amplify the ban homologous fragments from the genomic dna of brassica napus, b. chinensisl, b. juncea, a. thaliana and other cultural plants of cruciferae. the very similar pcr fragments were obtained from all the amplifications, which implicated that ban may be a conserved gene existing widely in the genomes of cruciferae. pcr fragments were cloned and confirmed by restriction enzyme digestion
參照擬南芥（ arabidopsisthaliana ） ban基因與cdna設計引物，對甘藍型、白菜型、芥菜型油菜，擬南芥及其它十字花科栽培品種的基因組dna進行pcr擴增，均擴增出與擬南芥ban基因擴增片段大小極其相似的dna片段，提示ban可能廣泛存在於十字花科植物中。 -
Based on the structure and function analysis of hirudin, a potent thrombin inhibitor, and some platelet aggregation inhibitors, which contain the recognition sequence argglyasp as their functional motif, two chimeric antithrombotic molecules were designed by introducing rgd sequence to hirudin cterminus. these chimera genes were constructed by pcr and inserted into the expression vector pet21a, the constructs were confirmed by restriction enzyme digestion and dna sequence analysis. these recombinant plasmids were transformed into
經限制酶消化和dna序列分析，證明兩種重組質粒與設計完全一致。由於rgd -水蛭素嵌合基因上游連接了金黃色葡萄球菌蛋白a spa的信號肽序列，在iptg誘導下兩種嵌合分子都獲得了分泌表達，表達產物主要集中在細胞周質空間。 -
It was confirmed by restriction enzyme digestion analysis that egfp fusion expression plasmids of scfvs were successfully constructed
的序列正確，經酶切鑒定證實成功地構建了綠色熒光蛋白基因融合表達載體。 -
It indicated that the chinese isolates belong to north american group. two pairs of different primers of orf7 were used to identify the genotype of prrsv isolates in china, and then compared with the reference isolates of north american and european serotype and modified live prrsv vaccine. the results further proved that prrsv prevalent in china belongs to b genetype. combining the restriction enzyme digestion patterns obtained from mini, hindi and sactt, we observed 2 distinct rflp patterns
在此基礎上，擴增各毒株的orf5基因，用mlu ， hinc和sac限制性內切酶切割orf5基因，通過這3種限制性內切酶獲得了各毒株的orf5基因限制性酶切圖譜，經rflp分析表明國內分離毒株與美洲型強毒株有著相同的rflp圖譜，而與疫苗毒的rflp圖譜存在明顯差異，進一步證明國內分離毒株的基因型屬於美洲型的強毒株。 -
Conclusion the enzyme digestion procedure is a stable and reliable method to obtain bovine retinal pericytes
結論：酶消化法原代培養牛視網膜周細胞是一種穩定、可靠的方法。 -
In this study, we designed a pair of primers based on the sequence of the upstream and downstream of chicken il - 2 gene. about 600 bp chicken il - 2 cdna fragment was cloned from cona - stimulated chicken splenocytes by reverse transcription - polymerase chain reaction ( rt - pcr ) and was subcloned into puc18 vector. recombinant clone was demonstrated by restriction enzyme digestion and dna sequencing. next, we construct recombinant plasmid pproex ? t - il - 2. the cdna of chicken il - 2 gene was subcloned into bamh i / hind iii sites of vector. the recombinant plasmid pproex ? t - il - 2 was transformed into e. coli dh5a and the bacteria was induced with iptg. it was demonstrated by sds - page and western blot that a 18kda protein which was equal to chicken il - 2 protein in molecular weight was expressed in e. coli dh5a. the expression level was up to 30 % of the total bacterial proteins. the purified protein was used to prepare the antibody against chicken il - 2 protein
經酶切鑒定及dna序列測定，該基因為雞il - 2基因，其序列與sundick等報道的完全一致。在此基礎上，我們把雞il - 2基因亞克隆到大腸桿菌原核表達載體pproex ~ （ tm ） ht中，構建重組表達質粒並進行確證性序列測定，重組質粒測序結果表明將編碼雞il - 2成熟蛋白的基因正確地插入到原核表達載體pproex ~ （ tm ） ht的目的位點。重組質粒轉化受體菌dh5後用iptg於37進行誘導培養， sds - page和westernblot分析顯示，表達的雞il - 2融合蛋白分子量約為18kda ，表達的融合蛋白經薄層掃描發現目的蛋白表達量約占菌體蛋白的30 。 -
The hinf i restriction enzyme digestion gave rise to restriction fragment length polymorphism ( rflp ) of the pcr products. when there were two dna fragments of 363bp and 305bp were produced from a pcr product, the strains were assumed to have a mutation at ser83, when three fragments of 363bp, 206bp and 99bp were produced, the strains were assumed to have no mutation at the 83or 116. the results indicated that certain mutation of ser 83 abolished a hinf i restriction enzyme site and may be detected as a rflp
本研究重點分析了16株菌對氟喹諾酮類（ fqns ）藥物的耐藥性，結果表明： 16株菌對諾氟沙星、環丙沙星、沙拉沙星、單諾沙星和氧氟沙星等（ nor 、 cip 、 sar 、 dan 、 ofl ）的耐藥率在31 . 3 - 56 . 3之間。 -
The recombinant was identified by dual enzyme digestion and the direction of cd40 / pcdna3 was analysed with t7 primer. after being packed by lipofectaminetm2000, the recombinant was transfected into b lymphocytes. cd40 expression on membrane, cell proliferation and antibodies concentration were detected with flowcytometry, mtt colorimety assay and el1sa, respectively
以脂質體為介質瞬時轉染健康人及sle患者b細胞系，利用流式細胞技術檢測膜cd40的表達情況，並利用mtt比色法檢測細胞的增殖能力， elisa法檢測培養液的ig濃度，以研究b細胞在cd40被抑制以後增殖能力、抗體分泌的改變。 -
E2 gene was cut with restriction endonuclease from the recombination plasmid t - e2, and subcloned into the expression vector ppic9 ' s mcs, which was identified by enzyme digestion and pcr amplification
將重組質粒t - e2的e2基因酶切后，亞克隆到表達載體ppic9上，經酶切和pcr鑒定，命名為ppic9 - e2 。 -
The ubi - sl - tocs fragment was taken out, inserted into the multi - cloning site of pcambia1300 vector, transformed into jm109 strain finally, positive colonies were screened on lb plate ( 60 g / ml kan added ). the result of pcr and enzyme digestion of plasmid proved that recombinatin vector was obtained ( named pcusaib4 and pcusaibu )
把擴增產物分別通過clai和bamhi酶切純化，連接到用clai和bamhi切去gfpml基因的中間表達載體pugfpocs中，轉化大腸桿菌jm109 ，在含amp （ 100 g / ml ）的lb抗性平板上篩選到了的陽性菌落。 -
In this study, pichia pastoris system had been utilized for expression of fmdv 2c3abc gene which aimed for establishing a sensitive and specific molecular dignosis method. first, 2c and 3abc genes were amplified individually from p2 and 3abc postive clones and ligated together using pcr method, then this 2c3abc product was cloned into pgem - t easy vector and transformed e. coli dh5a competent cell. a postive recombinant plasmid which contained whole 2c3abc gene had been confirmed by pcr, enzyme digestion and sequencing. after that, the 2c3abc gene was sub - cloned into ppiczaa expression vector and transformed e. coli dh5 a competent cell and selected by zeocin ? antibiotic. the postive recombinant expression vector was linerized and electro - transformed pichia pastoris smd1168 competent cell. a recombinant pichia pastoris had been obtained by zeocin ? antibiotics selection and induced with 0. 5 % methanol for target protein expression. the expression product was analysised by sds - page and western blotting assay. the result sh owed that 2c3abc gene was expressed successfully in pichia pastoris and the product was a 95ku fusion protein which could be recognized by anti - fmdv serum. the amount of target protein was over 15 % of the total bacteria protein by gel thin layer scanning analysis. this research had supplied materials for establishing a fmd diagnosis method to differentiate infected animals from vaccinated animals
首先，用p2和3abc陽性克隆通過連接pcr方法獲得目的基因並將其克隆到pgem - teasy載體上，並轉化e colidh5a感受態細胞中，經pcr 、酶切以及測序證明得到了完整的2c3abc基因，並與國內外參考序列進行比較分析。然後，將目的基因亞克隆于ppiczaa表達載體並轉化大腸桿菌dh5a ，以zeocin ~ （ tm ）抗性篩選陽性克隆，大量提取重組表達質粒並用pme酶線性化后電轉化入畢赤酵母smd1168感受態細胞，通過zeocin ~ （ tm ）抗生素梯度濃度篩選，獲得重組酵母用0 . 5甲醇誘導表達，通過sds - page電泳、 westernblotting分析，結果表明， 2c3abc基因在畢赤酵母中成功表達，其表達產物為一95ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。 -
After identification with both pcr and enzyme digestion methods, accurate recombinants were sent to detect their sequences
將經過pcr和雙酶切鑒定的重組克隆進行序列測定。 -
The gene fragments si and s2 were successfully amplified by pcr from plasmid pecob6 and were also successfully cloned into plasmid pbks ( + ). and the recombinant plasmids were selected through identification with pcr, enzyme digestion methods, and detection of their sequences
成功從質粒pecob6特異性擴增了s1和s2的基因片段，分別將二者克降到質粒pbks （ + ） ，經過pcr 、雙酶切攀定、序列測定等檢測。 -
The cyp51 genes cloned from both isolates of imazalil - resistant and imazalil - sensitive of p. digitatum were cloned into the pcb 1004 expression vectors, respectively. the direction and number of inserted copy were confirmed by restriction enzyme digestion
本研究構建了指狀青黴對抑霉唑敏感和抗性的兩個cyp51基因的pcb1004表達載體，並對基因的插入方向和拷貝數進行了分析，證明了兩個載體連接的基因均為正向連接和單拷貝插入。 -
In 1987, the notion of a splicing system was introduced by tom head in [ 3 ] as a mathematical model of restriction enzyme digestion and subsequence religation in the recombination of dna molecules
1987年， tomhead發表了一篇論文，引入拼接系統（ splicingsystem ）的概念作為限制酶與dna （脫氧核糖核酸）作用、進行dna重組的數學模型。 -
Methods : vascular endothelial cells, smooth muscle cells and fibroblast are respectively isolated from human umbilical veins by enzyme digestion and tissue plant methods, subcultured, purified and identified, etc. phase - contrast and electron microscopy was used to analyze the cells morphological characteristics
方法：分別採用酶消化法和組織塊法培養血管內皮細胞、血管平滑肌細胞及成纖維細胞，並進行三種細胞的傳代、純化、鑒定以及形態學觀察。 -
3. the amplified dna fragment was then ligated into the hindlll and ecori sites of pmg36e. the ligation mixture was transformed into jm109 for the initial cloning. the recombinant plasmid pmg36e / nisa isolated from jm109 transformants were analyzed by restriction enzyme digestion
將酶切並純化后的pcr擴增產物與大片段連接並轉化e . colijm109 ，在含紅黴素的lb平板上篩選到含有重組質粒的轉化子。 -
After repeated adherence and enzyme digestion, the cells attained the requirement of morphological purification with a purity of more than 95 %
經臺盼藍拒染計數，將細胞按1 10 ^ 6接種于培養瓶，傳代培養。 -
The expression vector ppiczaa with the poifn - a gene was constructed by restrict enzyme digestion and ligation, then transformed into e. coli jm109 in order to extract recombinant plasmid from jm109
將此基因與畢赤酵母分泌型表達載體ppicz a連接，構建ppicz a - ifn重組質粒，並用常規cacl _ 2法轉化大腸桿菌jm109進行擴增。 -
Restriction enzyme digestion analysis and pcr analysis showed that these two plant expression vectors constructed were correct. 49caixin, one cultivar of the brassica campestris l. ssp. chinensis, was transformed by vacuum infiltration method
通過真空滲入和花序浸漬法，將上述雙抗和單抗基因分別導入白菜不結球類型? ? 49菜心中，獲得了7棵抗性株。
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