epitope 中文意思是什麼

Main methods and results are as followed : 1 epitope analysis of agonist - binding region of nrla physicochemical properties and antigenicity of two agonist - binding regions of nrla were analyzed through bioinformatics : domain p1 containing 151 amino acid residues preceding the first transmembrane domain of the human nrla, domain p2 with 144 residues following the third transmembrane domain. four parameters including hopp - woods and kyte hydrophilicityjanin accessibility, karplus - schulz flexibility, and welling antigenicity were used to determine the antigenic sites, and prosite programme and chou - fasman method were employed to analyze their related sequence motif and the secondary structures

用goldkey軟體分別選取公認的hopp等與kyte等親水性參數、 jain表面可及性參數、 karplus - schulz主鏈柔韌性參數及welling抗原性參數對p1 、 p2兩個多肽片段進行參數分析。並採用通用的prosite程序與chou - fasman方法比較分析p1 、 p2多肽片段的氨基酸位點與二級結構特徵。綜合判定兩個多肽片段的抗原性及其位點，結果認為p2抗原性強于p1 。

This modification includes : ( 1 ) selecting two important molecules as candidates, ( 2 ) choosing a promiscuous t - cell epitope, and two b - cell epitopes or conserved amino acid sequences from the two important molecules, ( 3 ) connecting them adequately through analysis by the molecule designing software. therefore, the synthetic new antigen may interfere with the process of fertilization by multiple ways and its contraceptive effects may be enhancing. based on the molecule designing methods, the b - lymphocyte cell epitope of sperm / testis specific protein sp17 and cyritestin which interfere with fertilization in mouse, as well as the promiscuous th cell epitope of the ribonuclease ( rnase ) in bovine were selected

本研究以蛋白質分子設計的理論和方法研究避孕疫苗，將sp17和cyritestin關鍵表位和牛核糖核酸酶非選擇性th細胞表位合理組合，獲得新抗原- 35肽序列；並在合成、純化後分別與弗氏佐劑、免疫刺激復合物（ iscoms ）混合后免疫不同遺傳背景的雌性小鼠，觀察血清和生殖道內的特異性抗體滴度的動態變化、生育力的改變以及免疫后小鼠重要臟器的組織病理學改變：以及在ivf下，新抗原的特異性抗血清對精卵相互作用的影響及抗原在精子表面的特異性定位。

Value of 3b3 epitope concentration of synovial fluid in diagnosing early stage articular cartilage degeneration

3表位檢測對軟骨早期退變的診斷價值

To get in vivo evidences that apoplast calmodulin con 1d regulate plant growth and development process, a chimeric secretion form of calmodulin binding peptide, which contains a signal peptide, a calmodulin binding domain and a c - myc epitope was constructed. the chimeric gene was introduced into arabidopsis. it was expected that the overexpression of this chimeric protein could be secreted into cell wall and bound to apoplast calmodulin, which could reduce the apoplast calmoduin concentration to make an apoplast camodulin " antisense " plant. by observing the potential phenotype change of apoplast calmodulin " antisense " plant, the in vivo function of apoplast calmodulin on plant growth and developmental process could be speculated

但這些多是採用生理學手段和藥理學方法而得出的體外（ invitro ）實驗結果，為了取得質外體cam在植物生長發育過程中發揮重要作用的invivo實驗證據，根據動物中的一些研究方法，本實驗設計並構建了帶有信號肽、 cam結合肽（ can小肽） 、 epitope （ c - myc ）融合基因的載體，並將融合基因通過真空滲入法轉入擬南芥，預期過表達的融合蛋白將會被分泌到細胞外並與質外體cam相結合，這樣就會抑制質外體cam的功能，從而可以構建質外體cam的「反義」植株，通過觀察質外體cam 「反義植株」的表型改變，就可以推斷質外體cam在植物生長發育過程中的功能。

Studies of the crystal structure of endostatin have shown a compact globular fold, with one face particularly rich in arginine residues acting as a heparin - binding epitope, this site was recently shown to be involved in the inhibition of induced angiogenesis. experimental studies show that recombinant endostatin specifically inhibits the proliferation of endothelial cells in a dosedependent fashion. recombinant endostatin from bacteria is largely insoluble, but still efficient in arresting tu mor growth after injection into mice. intermittent therapy with recombinant bacterially produced endostatin reduces several experimental tumors, including lewis lung carcinoma, to a dormant state. no sign of drug induced resistance has been reported and, in the original study, the treatment dormancy appeared to persist even when therapy was discontinued. sowe regard endostatin as a promising anti - tumor drug

許多研究表明重組內皮抑素特異性抑制內皮細胞增殖，而且這種抑制作用呈劑量依賴性。細菌表達產物內皮抑素大部分以不溶形式存在，將這種混懸液注射治療老鼠仍可以抑制腫瘤生長。于小鼠皮下重復注射內皮抑素重組蛋白，幾乎完全抑制鼠lewis肺癌等多種腫瘤生長，並無耐藥性產生，即使中斷治療腫瘤也不再復發。

Presl ( 20 - 47 ) is one of the important epitope of hbsag, which has been pointed out to have relationship with infection of hbv. humanized antibody against presl with high affinity is a new way to block hbv

乙肝病毒表面抗原pres1 （ 20 - 47 ）表位是重要的乙肝病毒（ hbv ）表位，其識別肝細胞受體，並與hbv侵染人肝細胞有關。

These results demonstrate that nps in m3m4 loop is the b cell epitope recognized by mab363, which may be important for developing a practical immunization strategy against excitotoxic brain injury

、 ifn一y 、 il一4 、幾一6明顯增加，與對照組之間有顯著性差異，說明小鼠機體內產生了細胞免疫和體液免疫反應。

The 7mer peptide and 12 mer peptide selected from the peptide library elicited strong anti - vegf antibody immuno - reaction in mice. dna sequenseing showed that gwyydal and vasavfysalve mimic the discontinuous epitope of vegf by the 14 - 17, 21 and 21 25 amino acid residues respectively

兩者可能分別模擬了vegf分子的21 、 25位氨基酸和14 、 17 、 21位氨基酸通過折疊后在三級結構水平上形成的結合位點和vegf競爭結合受體。

Research for recombinant epitope antigens of hepatitis cvirus

重組丙型肝炎病毒表位抗原的研究

Identification and characterization of neutralizing antigen epitope of hepatitis e virus clustered in genotype

基因型毒株中和抗原表位的鑒定

In this experiment, to screen the epitope of e2 protein of classical swine fever virus ( csfv ) defined by the monoclonal antibody ( mcab ) all, which had been prepared in previous experiment, the mcab all was raised in mice and followed by purification, the concentration of protein was assayed by using the bca protein assay kit

本室利用e2基因疫苗制備了多株單抗，為e2的抗原表位研究提供了條件，我們以噬菌體隨機12肽庫分析鑒定了豬瘟病毒e2蛋白的抗原表位，為深入研究豬瘟病毒的抗原結構、制備更有效的診斷試劑和疫苗提供更多理論依據。

[ methods ] two phage - epitope libraries were screened with two anti - vegf neutralizing monoclonal antibodies - jh121 and vg189, the clones were tested by dot blotting and elisa

並通過硝酸纖維膜斑點印跡法進行陽性克隆鑒定。 elisa分析陽性噬菌體克隆與抗體的親和力。

Till now, a protein with 22kda in tartary buckwheat ( tb22kda ) has been identified as the major allergen, so further study of the corresponding epitope in virtue of immunological and molecular genetic methods will be helpful in understanding the molecular mechanism of allergenic reaction and finding proper ways of resisting irritability

Tb22kda蛋白是苦蕎中的主要過敏蛋白，對此蛋白中抗原決定簇免疫學和分子遺傳學的深入研究，將有助於了解過敏反應機制並設計抑制蕎麥過敏蛋白的表達或使患者脫敏的方法。

We paid our main attention to cloning tb22kda gene from tartary buckwheat, expressing the structure gene and getting the purified recombinant protein, and these provide foundation for father study on the relationship between structure and function of the allergenic protein tb22kda, the orientation of the corresponding epitope and the exploration of allergenic reaction mechanism

本研究的目的在於分離克隆苦蕎中的主要過敏蛋白（ tb22kda ）基因，並表達純化出其結構基因編碼的蛋白。從而為進一步研究過敏蛋白tb22kda的結構與功能，尋找其中的抗原決定簇，探討過敏原與其相應抗體相互作用機理提供依據。

By using western - blot, the fusion protein could not be react with antiserum to prrsv, whereas it presented a reactivity with swine antisera against prrsv and mab ge3 by elisa the results implied that the epitope might be one conformational epitope that was mainly composed of aa50 ~ aa55 domain on n protein

Western - blot分析表明表達產物與prrsv陽性血清沒有反應性，而elisa分析表明表達產物可與prrsv陽性血清和單克隆抗體發生反應。由此說明單克隆抗體ge3所識別的抗原表位可能是存在於n蛋白上以kphf為核心的構象型表位。

The purpose of this study was to elucidate the biological function of hnadcs and its relationship to renal disorders. methods : the biochemophysical characteristics and secondary structure of amino acid sequence of hnadcs were analysed via software on - line ( http / / expasy. pku. edu. cn / cgi - bin / ? protparam ), and the antigen epitope was predicated via software dnastar

鄭州大學2002年碩士畢業論文人鈉h被匹協同轉運目白zk合蛋白抗體的制擊皿其在矚臟的這達研究方法：首先利用網際網路上軟體mp ： llexpasypku ? edu ? cn7cgl七inlprotparam ，分析蛋白理化性質和二級結構，應用生物技術軟體dnastar預測hnadc3抗原表位。

The constructed vector was identified by sequencing. after induction by iptg, four polypeptides coding epitope expressed, respectively. research in this dissertation laid foundation for the development of nd - epitope - vaccine

經測序鑒定與原序列沒有突變及移碼后，經iptg誘導，有四段含表位的多肽獲得了表達。

Thirteen putative epitopes showing characteristics of antigenic epitope were found from the analysis information. using pcr, the nucleotide acid fragments encoding these putative epitopes were amplified, then cloned into the expression vector miske. the positive recombinant phage displying the epitopes were found out by using pcr, sequencing and the determination of phage plaque titer

運用goldenkey分子生物學軟體對prrsvbj - 4結構蛋白的抗原表位及其二級結構進行了分析和比較，從中篩選13段顯示表位特徵的氨基酸殘基序列，用pcr技術擴增相應的核苷酸片段，將其插入到噬菌體表達載體m13ke ，結果預測的13個表位可在噬菌體表面得以展示。

The humoral response induced by hybrid phage displaying candida albicans hspqo epitope in mice

展示白色念珠菌熱休克蛋白表位的雜合噬菌體誘導小鼠產生的體液免疫應答反應研究

In order to further investigate the role of axudl in human tumor carcinogenesis and the potential association between the axudl gene expression status and the stimulation of transforming growth factor beta in human cancers, the present study was performed in three aspects as follows : ( 1 ) cloning full length enconding region cdna of axudl and construction of eukaryotic vector that expression the fusion protein of axud1 and influenza virus hemagglutin ha epitope tag ; ( 2 ) exploring the time and dose effects of tgf - 1 on the expression - of axudl gene in hepg2 hepatoma cells and spc - a1 lung carcinomas cells, and studying the effects of overexpression of axud1 on the expression of cell cycle and apoptosis related protein in hepg2 hepatoma cells ; ( 3 ) construction and expression of human axudl in e. coli m15. the following main results and conclusions can be obtained from the present study : 1. the full length ecnoding region of human axudl cdna from human peripheral blood lymphocytes was successfully cloned using one step rt - pcr method, and constructed into a eukaryotic expression vector which can be expressed a ha - axud1 fusion protein with axud1 and influenza virus hemagglutin ha epitope tag. the recombinant plasmid was identified by polymerase chain reaction, restriction endonuclease maping and sequencing, this expression vector might be instrumental to further study the function of axud1 protein in tumor cells

為了進一步研究axud1在人類腫瘤發生中的作用及axud1基因的表達狀況與tgf -介導的信號通路的關系，本實驗研究分為三個部分： （ 1 ） axud1基因cdna全長編碼區的克隆和ha表位標記的axud1基因表達載體的構建； （ 2 ）探討肝癌細胞hepg2和肺腺癌spc - a1細胞中tgf - 1誘導的axud1基因表達的時間、劑量效應以及誘導表達的可能機理，並研究axud1的過表達對細胞周期和細胞凋亡相關蛋白表達的影響； （ 3 ） axud1原核表達載體的構建及其在大腸桿菌中的表達。本實驗的主要結果和結論如下： 1利用一步法rt - pcr成功地從人類外周血淋巴細胞中克隆出axud1基因編碼區cdna ，並將其構建入真核表達載體中，編碼的ha - axud1融合蛋白帶有流感病毒凝血素ha的表位標記肽段。