es cell 中文意思是什麼

es cell 解釋
胚性幹細胞
  • es : Es =【化學】 einsteinium.
  • cell : n 1 小室,單室;隔間,艙;〈詩〉茅舍;(單個的)蜂窩,蜂房。2 〈詩〉墓穴,墓。3 (大修道院附屬的...
  1. The application of dna fingerprint technique in monitoring the es cell chimeric mice

    Dna指紋圖在小鼠es細胞嵌合體鑒定中的應用
  2. Mouse ; restriction fragment length polymorphism ; dna fingerprint ; probe jl - 02 ; es cell

    小鼠限制性片段長度多態性dna指紋圖jl - 02探針胚胎幹細胞
  3. Spontaneous differentiation of es cell d3 line in vitro es cell d3 line via suspension culture can congregate small mass within 24 h, and large numbers of aggregates called embryoid bodies ( ebs ) gradually formed after 2 to 3 days of culture

    5 . es一d3細胞系的自主分化es一d3細胞懸浮培養, 24h后可聚集成小的細胞團,第2 ~ 3d時形成大量的類胚體( ebs ) 。
  4. Embryonic stem ( es ) cell lines isolated from primitive embryonic cells are highly undifferentiated and totipotent cells. in vitro, es cells can spontaneously differentiate into embryoid bodies ( ebs ) containing derivatives of three germ layers

    胚胎幹細胞( embryonicstemcell , escell )系是從早期胚胎細胞分離培養而建立的高度未分化的具有全能性的細胞系。
  5. 2. isolation and cloning of mouse embryonic germ cells of icr species primordial germ cells ( pgc ) were isolation from 8. 5 - 15. 5 dpc ( days post coitum ) embryos. eg cell lines with the characteristic of murine es cell line were established and continuously cultured to 6th passage

    Icr小鼠eg細胞的分離克隆研究本試驗以icr品系小鼠胚胎8 . 5 15 . 5dpcpgc為材料,經傳代培養,獲得能連續而穩定傳至6代的,具有胚胎幹細胞諸多特性的eg細胞系。
  6. Multi - locus dna fingerprint technique was used to check the chimerism of chimeric mouse generated by injecting es cells into blastocysts and to detect whether the chimeric mouse is a germ - line chimeras. the results indicated that : the multi - locus dna fingerprint with a new synthesized probe - jl - 02, has enough polymerism and good stability, and should be very useful to monitor the chimerism in different tissues of es cell chimeric mouse and to check whether an es cell line has the capacity to enter the germ line, especially when involving strains that can not be discerned with coat color or biochemical markers

    嘗試應用多位點dna指紋技術,檢測經過胚胎幹細胞es細胞途徑所獲得的嵌合體小鼠中es細胞在各種臟器中的嵌合情況檢測es細胞在嵌合體小鼠中是否實現種系傳遞。結果表明:採用新型的人工合成的寡核苷酸多聚體探針jl - 02探針的多位點dna指紋圖譜,具有足夠的多態性和很好的穩定性。
  7. The es cell clones decreased and disappeared gradually after 9 passages

    本實驗中的小鼠胚胎es細胞再傳至第九代后逐漸消失。
  8. Human es cell research in japan

    日本的人類胚胎幹細胞研究
  9. Labeling of three different mouse es cell lines with the green fluorescent protein

    在小鼠脊髓的表達和分佈研究
  10. A new method of deriving es cell lines from c57bl 6j mice was described

    報道一種新的建立c57bl 6j小鼠es細胞系的方法。
  11. Establishment of a c57bl 6j es cell line by conditioned media of rat myocardial cells

    用大鼠心肌條件培養基建立來源於c57bl 6j小鼠的es細胞系
  12. Es cell line ; c57bl 6j strain ; conditioned media of rat myocardial cells ; chimera ; immortalization

    Es細胞系c57bl 6j小鼠品系大鼠心肌條件培養基嵌合鼠永生化
  13. And hematopoietic progenitor assay was used to examine hematopoietic differentiation. the effect of mbmec - cm when combined with cytokines or mouse fetal liver stromal cell - conditional medium ( mflsc - cm ) on inducing es cells differentiation into hematopoietic cells was detected. the hematopoietic reconstitution of es cell - derived hscs was detected in vivo in irradiated recipient female mice

    實驗根據造血集落的形成、造血干祖細胞特異抗原和造血相關基因表達等方面檢測誘導生成的細胞是否含造血干祖細胞以及生成造血干祖細胞的數量,並且檢測誘導生成細胞的造血重建功能。
  14. The methods of evans and martin were changed slightly and used to isolate the mouse es cell in my experiment. in brief, the intact blastocysts were plated on sto feeder layer treated with mitomycin, and were cultured in the media supplymented with brl condition medium

    聯合evans和martin的方法,稍加改良來分離小鼠胚胎幹細胞,把昆明白小鼠完整的囊胚直接種植在經絲裂黴素滅活的sto飼養層細胞上,在含有brl條件培養基的es細胞培養液中培養。
  15. Hundreds of combinations of different types of antigens are possible, meaning that hundreds of thousands of es cell lines might be needed to establish a bank of cells with immune matches for most potential patients

    不同的抗原類型可能有數百種的組合方式,也意味著這可能需要建立一個含有數十萬種胚胎幹細胞株的細胞庫,以比對出與患者免疫相容性最高的幹細胞。
  16. There was no significant difference between the fusion rates of reconstructed embryos. the cleavage rate of embryos reconstructed with c57bl / 6j oocytes was significant high than that of kunming albino, but the former only developed to 4 - cell stage ; the later could develop to blastocyts. it is implied that the oocytes of inbred mouse cannot support the long - term development of kunming es cell

    兩種重構胚的融合率之間沒有顯著差異( p 0 . 05 ) , c57bl 6j重構胚的卵裂率( 60 . 5 )明顯高於昆明白小鼠( 33 . 7 ) ,但是前者只發育到4細胞期,而後者可以發育到桑椹期和囊胚期( 2 . 3 ) 。
  17. We used the icr and kunming mice and got the embryos of 3. 5dpc by means of superovulation, then cultured the embryos on the feeder layer derived from icr or kunming mice, dispersed the inner cell masses ( icms ) or es cell colony in the appropriate time. in this period, we analized the effects of feeder layer, trypsin and embryos isolated from uteri of different varietal mice on the es cell lines

    以icr小鼠和昆明小鼠為實驗對象,採用超數排卵的方法獲得小鼠胚胎,培養在用絲裂黴素c處理過的icr小鼠和昆明小鼠mef細胞飼養層上,選取適當時間離散增殖的icm和類es細胞集落,分析了在小鼠es細胞建系過程中,飼養層細胞、消化液及不同品系來源的胚胎的影響。
  18. After 4 days of growth, the intact inner cell mass ( icm ) were separated with 0. 05 % trypsin - edta and replated on feeder layer in dmem containing 15 % serum, 0. 1mmol / l nonessential amino acids, 0. 1mol / l 2 - mercaptoethanol, 2mmol / l glutamine, 100 units / ml of streptomycin and 100 units / ml of penicillin. after 4 - 6 days 5 es cell colonies were selected and expanded in which alkaline alkaline phosphatase was detected

    ( 2 )小鼠囊胚或內細胞的培養和es細胞的分離培養了156個不同品系的小鼠囊胚,經過3 ? 4天培養后,將增殖出來內細胞團用機械法結合胰酶- edta處理,離散后培養于mef飼養層上, 4 6天後有5例出現了es細胞樣集落。
  19. One chimaeric mouse was produced by injection of es cells into blastocyst of c57bl / 6j. the chimaeric rate of fur colour was about 15 %. fl generations of germ - transmission derived from es cell were not obtained when chimaeric mouse mated with kunming albino mice and c57bl / 6j mice

    將胚胎幹細胞注射到c57bl 6j小鼠的囊胚腔內,移植后產生了一隻wk2 -胚胎幹細胞的雄性嵌合體鼠,毛色嵌合率為15左右,成熟後分別與c56bl 6j和昆明白雌鼠交配,均未得到來自es細胞的後代。
  20. Present research proved that it is reasonable to explore es cell committed induction pathway by transfecting special transcript factor, maker gene or relative cell differentiation factors combined with reporter gene and selecting method with inducer

    1作載體,電轉方法對es細胞進行轉染,以18進行篩選。 2 . rt一pcr方法鑒定ibob基因的插人和表達。
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