feeder layer 中文意思是什麼
feeder layer
解釋
滋養層-
Es - d3 cells differentiated into ebs only when depleted of feeder cell layer by being cultured three generations in the presence of leukemia - inhibitory factor ( lif )
結果表明： eso3細胞在含有lif的培養液中脫離飼養層培養3代以上才能形成ebs 。 -
Part 1 : the culture and identification of es - d3 cells and the study of the efficiency of eb formation from es cells when grown on mef feeder layer in es culture medium or cultured in es culture medium supplemented with lif 1000u / ml, es - d3 cells being used in our experiments formed normal clones, expressed akp and kept their normal karyotype over many passages. the in vitro and in vivo differentiation experiments showed that es - d3 cells could differentiate into variety of cell types derived from three primary germ layers
結果顯示： eso3細胞在小鼠胚胎成纖維細胞上和或含白血病抑制因於億f ）的es細胞培養液中形成典型的胚胎幹細胞克隆，堿性磷酸酶染色結果為強陽性，具有正常二倍體核型以及具有在體內外分化為三個胚層來源的組織細胞的潛能，而且具有形成種系嵌合動物的能力。 -
The quality of feeder layer is affected by a lot of factors, such as animal breed, culture medium, passages in vitro and experiment condition, etc. as to the production of feeder layer, there are a few reports about morphological and histologic change when of embryonic body fibroblast when culturing in vitro and cryopreservation, so kunming mouse were chosen as experimental animals and morphological and histologic changes were studied in course of its embryonic body culturing. we expect to offer theoretical foundation to our laboratory for setting up feeder layer storehouse. at the same time, the feasibility of myocardium tissue culturing with fibroblast layer altogether was studied so that established foundation for studied the biological characteristic of heart outside body
小鼠胚體成纖維細胞的培養是制備飼養層的重要途徑，其制備、傳代及冷凍保存均有不同的研究報道，飼養層的質量受許多因素的影響，如動物的品種、培養液、所傳代數及實驗條件等，關于飼養層制備過程中的胚體細胞培養、傳代、冷凍后的細胞形態、組織學等方面的研究報道很少，故本實驗以昆明小白鼠為實驗動物，研究其胚體培養過程中細胞的形態學、組織學等方面的變化，以期為本實驗室建立飼養層細胞庫提供理論依據，同時探討心肌細胞和成纖維細胞層共培養的可行性，以期為心臟生物學特性的體外研究奠定基礎。 -
The methods of evans and martin were changed slightly and used to isolate the mouse es cell in my experiment. in brief, the intact blastocysts were plated on sto feeder layer treated with mitomycin, and were cultured in the media supplymented with brl condition medium
聯合evans和martin的方法，稍加改良來分離小鼠胚胎幹細胞，把昆明白小鼠完整的囊胚直接種植在經絲裂黴素滅活的sto飼養層細胞上，在含有brl條件培養基的es細胞培養液中培養。 -
The primary primordial germ cells is obtain from the human embryos after 4 - 8 weeks of pregnancy. after mechanical desection and enzymic digestion, the cells were cultured on mouse embryonic flbroblast or human embryonic flbroblast feeder layer inactivated by mitomycin. the medium contains several cytokines : lif ( leukemia inhibitory factor ), bfgf and foskolin
從4 ? 8周的流產胎兒的原始生殖嵴部位分離到原始生殖細胞，經過機械和化學方法的解離后培養於事先用絲裂黴素處理過的小鼠胚胎成纖維細胞或人胚胎成纖維細胞飼養層上，培養基中添加了lif ， bfgef ， foskolin等細胞因子，此後大約每7天左右傳代一次。 -
We propose a combined slf method to extrapolate feeder load growth by using feeder ' s history peak value and the merits of gray theory and genetic programming ( gp ). at first, we adopt load transfer coupling method to correct load history and its error for load transfer. secondly, we get the real power - supply area by using layer overlap analysis, based on practical feeder path and distribution gis map layer
將gis的空間信息分析功能應用於配網空間負荷預測的研究：綜合利用灰色理論及遺傳規劃（ geneticprogramming ， gp ）的優點，提出了一種根據饋線的歷史峰值負荷進行外推的組合slf法：首先採用負荷耦合回歸法來修正負荷歷史，消除由於負荷轉移引起的誤差；然後根據實際饋線路徑和配網gis圖形分層，運用圖層疊加分析得到饋線的實際供電范圍；接著採用灰色關聯度聚類方法對饋線負荷增長曲線進行聚類分析；最後採用gp來對灰色聚類結果進行符號回歸，分別得到每一類曲線的最佳擬合曲線形式。 -
With the increase of passage, the total of colonies decreased, but the number of classical nest - like or island - like eg cell colonies relatively increased. the growth of eg cells was dependent on the feeder layer
傳代培養的eg細胞對飼養層有依賴性， icr品系小鼠pgc細胞和由其傳代后獲得的eg細胞呈明顯的群聚性，以單個存在狀態的較少 -
We used the icr and kunming mice and got the embryos of 3. 5dpc by means of superovulation, then cultured the embryos on the feeder layer derived from icr or kunming mice, dispersed the inner cell masses ( icms ) or es cell colony in the appropriate time. in this period, we analized the effects of feeder layer, trypsin and embryos isolated from uteri of different varietal mice on the es cell lines
以icr小鼠和昆明小鼠為實驗對象，採用超數排卵的方法獲得小鼠胚胎，培養在用絲裂黴素c處理過的icr小鼠和昆明小鼠mef細胞飼養層上，選取適當時間離散增殖的icm和類es細胞集落，分析了在小鼠es細胞建系過程中，飼養層細胞、消化液及不同品系來源的胚胎的影響。 -
It was found that mef by treatment with mitomycin c has no significant difference as feeder layer as with y irradiation
研究發現採用絲裂黴素c或者射線處理mef作為飼養層沒有明顯的差異。 -
Mef were inactivated by treatment with 10ug / ml mitomycin c for 2. 5 - 3 hours or y irradiation 50gy / 75gy dose and grew in dmem as feeder layer to help mouse es cells and human pgcs
採用10 g ml的絲裂黴素c或劑量為50gy和75gy的射線抑制mef有絲分裂，製作用於培養小鼠es細胞和人pgcs的飼養層。 -
After 4 days of growth, the intact inner cell mass ( icm ) were separated with 0. 05 % trypsin - edta and replated on feeder layer in dmem containing 15 % serum, 0. 1mmol / l nonessential amino acids, 0. 1mol / l 2 - mercaptoethanol, 2mmol / l glutamine, 100 units / ml of streptomycin and 100 units / ml of penicillin. after 4 - 6 days 5 es cell colonies were selected and expanded in which alkaline alkaline phosphatase was detected
（ 2 ）小鼠囊胚或內細胞的培養和es細胞的分離培養了156個不同品系的小鼠囊胚，經過3 ? 4天培養后，將增殖出來內細胞團用機械法結合胰酶- edta處理，離散后培養于mef飼養層上， 4 6天後有5例出現了es細胞樣集落。 -
Result : 1 ) dmem : f12 would enhance the proliferation of hpfl, especially present with a concentration of 20ng / ml eof ; pref feeder layer can defer the differentiation of hpfl in vitro, but collagen - vi and gelatin can not do
研究結果： 1 ）含有egf的dmem ： fiz對于hpfl的體外增殖明顯優于其它培養液；在不同的培養基質中， pref飼養層優于明膠和膠原，可促進hpfl的生長，並能延緩hpfl在體外的分化。 -
If alb + ck - 19 - cells would be induced into alb + ck - 19 + cells, we can prove that hpfl contain bipotential stem cells. 3 ) primary hpfl seeded on pref feeder layer were collected and engrafted into the spleen of sced mice to explore the propagation of hpfl in vivo
3 ）收集在原代大鼠成纖維細胞（ pref ）飼養層上培養的原代hpfl ，注人scto鼠脾臟，觀察hpfl植人體內的增殖發育情況）將分離的大鼠ed13 -
The enhancing factors of eb formation were studied. fifty thousand es - d3 cells per milliliter were incubated in dmem - high glucose supplemented with 20 % fbs and 2mm glutamine. the influencing factors which included hyclone fbs and homemade green season fbs, feeder cell layer, culture containers and 3 - mercaptoethanol ( - me ) were investigated
以5x10 』加的eso3細胞種入含20胎牛血清和zinm谷氨酚胺的高糖dmem中，觀察進口胎牛血清和國產胎牛血清、飼養層、培養器皿以及卜硫基乙醇對es士3細胞形成的4debs數量的影響。 -
The combination of rh - cm with mitotically inactive mef feeder layer, was the best culture system
其中以mef作飼養層，添加rh - cm培養基一組效果最好。 -
The clonies of es cells were flatter on the mitomycin c - inhibited sto fibroblasts, not easily differentiated from the feeder layer cells surround them, than that on the mef in dmem supplemented 15 % fbs. 5
以sto細胞作飼養層，添加15 fbs ，在倒置顯微鏡下觀察， es - d3細胞不易與周圍的sto飼養層細胞區別，形成的集落較平坦，生長狀態不如mef飼養層好。 -
We also investigated the factors including feeder layer cells, growth factors and complements, which may affect the cultivation and differentiation of these cells in vitro. the main contents as follows : ( 1 ) isolation and culture of mouse embryonic fibroblast ( pmef ) and preparation the feeder layers
主要內容包括： （ 1 ）小鼠胚胎成纖維細胞（ mef ）的分離培養和飼養層細胞的製作以妊娠13 . 5天或14 16天的小鼠為材料，建立了mef分離培養體系。 -
The blastocyst was obtain from the mouse after 3. 5 days of pregnancy, and was cultivated on the mouse embryonic flbroblast feeder layer. the blastocyst usually attach to the feeder layer after 48 hours, then the inner cell mass began to grow and form a big cell mass of embryonic stem cells. these cells can form cell clones with the conformation of embryonic stem cell
囊胚一般在48小時后貼壁並脫去透明帶，由囊胚中的內細胞團增殖形成一個胚胎幹細胞球，待其生長至一定程度后對其進行酶解傳代，在傳代后的細胞中重新生成es集落形態的細胞集落，在集落生長至一定程度后再進行傳代，此後大約每5 ? 7天傳代一次。 -
Medium which is serum - free or in the low concentration serum inhibit the proliferation of cells. a certain degree of increase serum concentration can promote the growth rate as well as prolong the life. the feeder layer cells were provided by mef, mue ( mouse uterus epithelium ) and rmc ( rat myocardial cells )
Mef和es細胞體外培養血清濃度以15 20為宜，試驗中以mef 、 mue 、 rmc作飼養層，結果表明：以12 . 5dpc的mef為飼養層，無論是在小鼠es細胞的原代培養，還是在克隆傳代過程中，效果都是最佳的。 -
( 7 ) in order to establish a feeeder - free system in porcine eg culture, we put pgcs on sto feeder layer, on sto extracellular matrix or on 0. 1 % gelatin in brl - cm, and the results showed that when pgcs cultured on sto feeder, the brl - cm had a positive effect on keeping the undifferent state of eg colonies
（ 7 ）對非飼養層培養系統培養豬pgc進行了初步探索。實驗結果證明，以sto為飼養層，培養液中加條件培養基對豬eg細胞保持不分化狀態有積極作用，但培養板底鋪有sto基質或明膠，即使用條件培養基也沒有獲得典型的eg集落。
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