fluorescence cell 中文意思是什麼

fluorescence cell 解釋
熒光試池,熒光細胞
  • fluorescence : n. 熒光（性）。
  • cell : n 1 小室，單室；隔間，艙；〈詩〉茅舍；（單個的）蜂窩，蜂房。2 〈詩〉墓穴，墓。3 （大修道院附屬的...
  1. The changes of the expression of gfp in biu - 87 cell that induced by the aconitine and hab toxins, gtx were detected with fluorescence microscope, and quantitatively measured with image - pro plus software

    經誘導，抗性細胞發出較強的綠色熒光，表明重組質粒pegfp - c - fos在biu - 87細胞中成功表達。
  2. [ result and discussion ] 1. combination between 2 - amac labeled oligochitosan and macrophage : 2 - amac - oligochitosan first bound the cytomembrane of macrophage, and then diffused in the whole cytoplasm, at last entered the nucleolus and diffused in the whole cell. fluorescence intensity increased with time

    2 -氨基吖啶酮標記的殼寡糖與巨噬細胞的結合情況： 2 -氨基吖啶浙江大學碩士學位論文酮標記的殼寡糖先與巨噬細胞膜有結合，然後分佈於整個細胞質，最後進入細胞核，隨時間的進展而呈現了一個內在化過程。
  3. The cell microarrys were dyed with trypan blue, wrights, three colors fluorescence and papanicolaou strained. results leukocyte samples from 20 all patients showed predictably and distinctly different dot patterns from samples from 20 normal subjects

    將雜交后的細胞晶元進行胎盤蘭染色、瑞氏染色、 cd3 cys cd4 fitc cds rpe三色熒光染色、巴氏染色等並觀察結果。
  4. Observe gvhd for 60 days. the results showed that mouse lymphoid cells were present in the sd rats detected by fluorescence activated cell sorter ( facs ) for morn than 90 days. the chimeric rates were 18 % - 45 %, 7 % - 23 %, 2 % - 8 % on day 30, 60, 90 respectively

    隨著時間的推移，嵌合率逐漸下降。骨髓移植后60天及90天的嵌合率分別為7 - 23 ， 2 8 。
  5. Recombination gene was cut - down and introduced into the nuclei of oocytes or the cytoplasm of goldfish at one - cell stage via microinjection. the results as follows : ( 1 ) fluorescence was observed from embryo under suitable uv light after microinjection 36 hours. the fluorescent ratio of gastrula embryo period was up to 25 %

    採用顯微注射法將這種重組基因轉化1細胞期的金魚受精卵，實驗結果如下： （ 1 ）顯微注射后，根據胚胎發育分期，胚胎在顯微注射后36小時開始能在紫外燈下觀察到熒光，原腸期發熒光的胚胎比例為25 ，後期發育熒光率逐漸下降，肌肉效應期后又相對穩定。
  6. We used fission yeast schizosaccharomyces pombe ( s. pombe ), an unicellular eukaryotic organism, as research material. electroporation was adopted to load ca2 + fluorescent indicator into yeast cell and under the laser scanning confocal microscopy ( lscm ), we observed cytosolic ca2 + distribution and relative content as well as fluorescence intensity of gfp - cam in different phases of cell cycle of yeast cell. flow cytometry provided a way of determining the relative dna content of populations of fission yeast

    本文以單細胞的真核模式生物裂殖酵母（ schizosaccharomycespombe ）為研究材料，通過激光掃描共聚焦顯微鏡觀察酵母細胞胞質內游離ca ~ （ 2 + ）的分佈及相對濃度，以及不同周期時相細胞中gfp - cam的熒光強度變化，並採用細胞流式法對酵母細胞的相對dna含量進行測定以確定細胞所處周期時相。
  7. The loop sequence of mb1 and mb2 were the anti sense and sense sequence ofing1, respectively the sequence of mb3 was a piece of ssrna sequence in tobacco mosaic virus, which had no analogical to human gene. mbl was the most suitable probe because mbl had the highest fluorescence enhancemen after hybridizing wtth rna extrated froin normal cell

    第三章，根據一種常見的病毒煙草花葉病毒（ tmv ）的核酸序列設計了分子信標熒光探針，由於tmv的遺傳物質是rna ，分子信標又具有很高的特異性和靈敏度，因此感染了病毒粒子的植物葉片在經過簡單處理后，可用分子信標檢測葉片上。
  8. Anti - if - protein antibodies were used as probes for immuno - fluorescence, and the reactions of them with different parts of the cell were detected, which suggested the possibility of the existence of the intermediate - like filaments in the cytoplasm. those proteins homologous to the antibodies distributed regularly in the protoplasm. to characterize the corresponding proteins, sds - page and immunoblots were utilized. the 21, 23, 33 and 68kd proteins were distinguished among the diverse protein constituents of the cell. some of these proteins also showed the cross - reactivities with anti - if - proteins antibodies derived from higher organisms. these two evidences both contributed to the homology of some proteins in

    以抗中間纖維蛋白抗體作為探針進行免疫熒光實驗，得到細胞內不同部位的陽性反應，暗示原生質中可能存在類中間纖維。這些同源蛋白的胞內分佈具有一定的規律性。進一步採用sds - page和免疫印跡技術研究它們的生化性質，發現4種主要蛋白明顯有別于胞內其他蛋白組分。
  9. 3. observe the binding of oligochitosan labeled with 2 - amac with macrophages under confocal laser microscope and analysis the fluorescence intensity by flow cytometer using the cell quest software

    在激光掃描共聚焦顯微鏡下觀察2 -氨基吖啶酮標記殼寡糖與巨噬細胞的結合，用流式細胞儀分析熒光強度。
  10. The self - segregation behavior of amphiphilic copolymer on pdl - la scaffold was investigated via fluorescence - labeling technique. the modified scaffold with hydrophilic surface will not only favor the penetration of cell suspension and culture medium, but also provide the microenvironment for the growth of cells with the peo spacer combining amino acid ( rgd ) structure. according the above result, the cytocompatibility test was also performed on pdl - la 3d scaffold modified by amphiphilic copolymer with alkaline amino acid end

    這種親水表面不僅有利於細胞懸液和培養介質的進入，並可以通過peo橋聯的氨基酸（ rgd ）為細胞在三維多孔支架內的生長提供類細胞外基質環境；根據以上結果，本文對堿性氨基酸為peo鏈端基的兩親共聚物-氨基酸類細胞外基質修飾的聚乳酸三維支架進行了細胞相容性的測試。
  11. The condition of profiles in outer station did n ' t change much in spring cruise, but showed more variable in near - shore stations when observed in different time. fluorescent characteristic per cell can be obtained by flowcytometric analysis. based on fluorescence data of synechococcus of all stations, two distinctly pigment - containing cell types coexisting can be found in some stations of east china sea, which located in all depth of p3, mixlayer of e7, 40 - meter depth of e6 of autumn cruise and in mixlayer of p2 of spring cruise

    通過對流式細胞計測量的細胞熒光結果來看，在秋季的p3 、 e7整個混合層、 e6站40米層，春季的p2站均發現有兩群不同色素含量的聚球藻（ high一pe和low一pe ）共存現象，極有可能分別屬于不同品系，春季共存站位位置比秋季時更靠外，表明在秋季p3 、 e7等站位的共存是季節性現象，可能與此季節黑潮次表層水沿陸架坡涌升入侵到中陸架有關，水團的運動及混合使從外海遷移而來的high一pe與近岸的low一pe得以共存，在春季，由於長江沖淡水的日漸強盛，在中陸架區的共存區域有所外移。
  12. To observe tsarg2 fusion protein expression in mammalian cell, pegfp - nl / tsarg2 fusion plasmid was constructed and transiently introduced into cos7 cell by liposome transfection. under the fluorescence microscope, the green fluorescence produced by pegfp - nl / rsarg2 was detected on the nucleus of cos7 cells after 24 hours post transfection, while the fluorescence produced by pegfp - nl was detected through the cells

    同時將21大、 35天、 49大、 280天小鼠睪丸進行組織石蠟切片，結果表明小鼠翠丸在第21天可見精子細胞，到第35天，精子生成的最後階段開始發生，部分牛精小管中可見少量成熟的精於。
  13. At that time, cytosolic fluorescence intensity decreased to normal level, which shows that most of cells get through the gl / s point and enter the log phase. when cultured in medium that neucl was omitted, most of the cells were synchronized at gl stage of cell cycle. with flow cytometry, we found that cytosolic cam content of gl cells was higher than that of normal cells at log stage

    在激光掃描共聚焦顯微鏡下觀察不同周期時相裂殖酵母細胞中cam的濃度及分佈變化，結果表明，分裂期細胞總體熒光強度強于間期細胞；而對同一細胞內熒光強度的分析說明，間期細胞的熒光主要分佈於胞質中，細胞核內則分佈較少；而正在進行有絲分裂的細胞內熒光主要集中於赤道板處；剛完成有絲分裂的細胞內熒光則相對集中於兩端或其中的一端。
  14. The obtained recombinant - 5 - htr plasmid was tranfected into human liver cancer smmc - 7721 cells. all data suggested the expression of plncx - atr could condense cell nucleus and increase nuclear fluorescence intensity, effectively repress the telomerase activity, cell growth and cell proliferation, and induce cell apoptosis

    反義重組質粒plncx - atr轉染人肝癌smmc - 7721細胞，結果發現plncx - atr的表達有效地封閉或抑制肝癌細胞的端粒酶活性，使細胞的生長和增殖受到抑制，細胞體積變小、核緻密、核熒光強度增強，且促進其凋亡。
  15. Measurement of cell calcium ( review ) methods for the intracellular ca2 + measurement such as aequorin, metallochromic indicators, ion - selective electrodes and fluorescent ca2 + indicators, particularly the fluorescent ca2 + indicators were reviewed in detail. 2. synthesis of new fluorescence ca2 + indicators and study on their properties tow new fluorescent ca2 + indicators stdin and stdbt have been designed and synthesized and their structures, spectral and biological properties have been studied thoroughly

    2 . 5一ht誘導胃底平滑肌細胞鈣火花的研究以合成的胞漿特異性caz +熒光探針stdhi一am標記細胞，採用激光共聚焦線掃描技術，首次觀察到5一ht誘導胃底平滑肌細胞胞內鈣釋放的最基本單位一一j塑必嘗華盆翔望嘗『一盈些沁（ elementaryevents ） ， 「鈣火花（ ealeiumsparks ） 」現象。
  16. According to the gene sequence and secondary structure of hcv ns5b, we design the sirnas targeting ns5b gene following with the requirement for sirnas design from tuschl et. al and synthesize it from dharmacon company ; hepg2 cell stably expressing ns5b - egfp protein was trasfected by synthesized sirnas with electroportion, the non - transfected cell and non - specific sirnas transfected cell are c onsidered as control group ; inhibitory effect of sirnas was investigated by fluorescence microscope with dapi dyeing and by semi - quantitative rt - pcr

    然後根據dsrna設計原則，結合nssb基因的序列特徵，藉助生物信息學軟體設計了針對nssb基因的sirnas ，並交由公司化學合成；電穿孔法轉染上述穩定轉染的細胞克隆，同時分別以非特異的sirnas轉染組和空白轉染組為對照， dapi染色后通過熒光顯微鏡和內標化rtpcr檢測，初步證實了化學合成的sirnas可以特異阻斷nssb基因的表達。
  17. By fluorescence real - time pcr, we found that the expression quantity of jak2 increased along with the development of oocyte we also compared the quantity of jak2 in gv oocytes and 2 - cell embryos nuclear. the quantity of jak2 significantly reduced in 2 - cell embryo nuclear compared with gv oocytes

    結果發現，隨日齡的增加， jak2的表達量也隨之增加。本試驗還比較了jak2在gv期卵母細胞和2 -細胞胚細胞核中量的變化。結果發現2 -細胞胚核中jak2的量較gv期明顯降低。
  18. Green fluorescence can be visualized in leaf epidermal cells and root epidermal cell in few of transgenic toreniafournieri

    只有極少數的轉基因植株在表皮細胞和根皮層細胞中有目的基因的表達。
  19. Research on the ratio method of measuring intracellular ca2 + concentration with ratio fluorescence imagemaster was reported in the last chapters, which include the folio wings : ( 1 ) the ratio experiment system of measuring intracellular ca concentration with ratio fluorescence imagemaster was established ; ( 2 ) the primary culture mode of suckling mouse cardiac muscle cell was established and the ca2 + concentration in suckling mouse cardiac muscle cell was measured. after adding ne ( norepinephrine ) to cardiac muscle cell, the relative fluorescence intensity was dramatic increased

    本文後半部分主要圍繞用比率熒光光譜儀測量細胞內游離ca ~ （ 2 + ）的研究進行了以下工作： （ 1 ）建立了比率熒光光譜儀測量細胞內游離鈣離子濃度的實驗系統； （ 2 ）建立了乳鼠心肌細胞原代培養的模型，並對培養細胞進行了細胞內ca ~ （ 2 + ）濃度的測量。
  20. The cellular localization of kvl. 2 and the co - localization of kvl. 2 and vegf receptors in the rat brain in rat cerebral cortex, triple - fluorescence labeling for kvl. 2, nse, and gfap showed that kvl. 2 immunopositive labeling was predominantly seen on the membrane of the cells co - stained with nse, a specific neuronal marker. only few kvl. 2 positive labeling cells were co - stained with gfap, an astrocyte glial cell marker

    大鼠腦內kv1 . 2蛋白的細胞定位及其與vegf受體間的共存關系大鼠大腦皮層kv1 . 2 、 nse 、 gfap免疫熒光三標結果： kv1 . 2蛋白分佈在細胞膜上，大部分kv1 . 2免疫陽性細胞表達有nse ，只有在少量kv1 . 2陽性細胞上表達有gfap 。
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