fluorescent assay 中文意思是什麼

fluorescent assay 解釋
熒光測定
  • fluorescent : adj. 1. 熒光的，發熒光的。2. 外表華麗的；光輝四射的。3. 〈美口〉容光煥發的。n. 〈美口〉熒光燈。
  • assay : n 1 化驗；分析；鑒定，測定，驗定。2 被分析物，被化驗物。3 化驗結果，化驗報告。4 〈古語〉企圖，嘗...
  1. Aliquots of cells were mixed 0. 15 % mg / ml fb - 28, and kept at 4c for 30min, fusion assays were conducted : fluorescence was measured immediately at regular time - points with fluorescence spectrophotometer with an excitation wave length of 560nm and emission wave length of 590nm. the percentages of membrane fusion was calculated. by monitoring fusion using the r18 assay, we found that the fluorescent brightener 28 influenced membrane fusion of virus and midgut epithelia cells

    此外，採用分子探針r18 （熒光標記物）標記病毒囊膜，體外分離中腸上皮細胞，將標記的病毒粒子與離體中腸上皮細胞混合后保溫，病毒吸附zh后，通過檢測熒光的變化來監測病毒粒子與上皮細胞的融合。
  2. The equipment shall be composed of : microplate reader for elisa ( or analyser elisa, analyser enzyme fluorescent assay ), microplate washer, micropipette, water - bath or temperature cabinet, refrigerator, shaker for microplate. etc

    儀器設備組合應為：酶標儀（或酶免分析系統、酶免熒光分析儀等） 、洗板機、加樣器、水浴箱或溫箱、冰箱、酶標板振蕩器等。
  3. The specific band was excised from the gel and injected into mice. the antiserum was collected from the immunized mice and used for indirect fluorescent assay ( ifa ) with chicken embryonic firoblast ( cef ) infected by mdv

    誘導菌體的裂解物經12的sds聚丙烯凝膠電泳（ sds - page ）和western - blot試驗驗證，得到大小為43kd的融合蛋白，與預期大小一致。
  4. Detection of salmonella in foodstuffs with enzyme linked fluorescent immuno - assay

    利用熒光免疫測定法檢測帶酵素食品中的沙門氏菌屬
  5. Firstly, a reverse hemolytic plaque assay ( rhpa ) was used to scale testosterone secretion of leydig ' s cells in single cells level. sequentially, star mrna expression was detected with fluorescent in situ hybridization ( fish ) at the same cells

    本研究首先採用逆向溶血斑法，鑒定單個間質細胞睪酮分泌上的差異，然後，在被鑒定的同一間質細胞上進行原位雜交（ fish ） ，以便在單個細胞水平上確定間質細胞是否存在功能及star基因表達的異質性。
  6. Under the flow cytometry assay, the effect of sfamnpv on mitochondrial membrane potential ( m ) was investigated by rh - 123 staining of cells. the rh - 123 fluorescent intensity in sl - 1 cells was reduced in time - dependent manner. the results of confocal laser scanning microscope confirmed that sfamnpv was able to reduce awm

    Westernblotting檢測線粒體cytoc和bcl - 2含量的的變化，結果顯示抗凋亡蛋白bcl - 2在病毒的感染過程中含量是逐漸降低的，即病毒的感染導致bcl - 2基因表達的下降。
  7. The results of inhibition of t cells activation assay indicated that activation of t lymphocytes which mainly recognize the different compatibility of class ii mhc molecules was inhibited in different degree due to the inhibited inducible expression of class ii mhc molecules on hela by recombinant adenoviruses containi ng cii ta antisense fragment, showing as decreases in the positive rate and mean fluorescent intensity of cd25 and cd69 expression, which are both signs of activated t cells

    T細胞激活抑制實驗表明：由於重組腺病毒攜帶的反義c11ta片段，不同程度地抑制了hela細胞誘導型mhc二類分子的表達，使主要識別mhc 11類分子相容性不同的t淋巴細胞活化受到不同程度的抑制，表現為t細胞激活標記物cd25和cd69表達的陽性率和平均熒光強度均下降，其中一例cd25陽性率降低17 l ，平均熒光強度降低50
  8. Multiple kinase assay was performed to examine pkc ^ mapk. tpk activity in the transfected cells. meantime, pegfp - sh2a vector was also constructed and the cells transfected with it were examined by fluorescent microscopy. the expression of sh2a gene was examined under different concentration and time of bfgf as a stimulating factor

    1 sh ，利用脂質體轉染肝癌bel7402細胞人os7細胞，檢測pkc 、 mapk 、 tpk活性的改變；流式細胞儀檢測細胞增殖；另構建pegfp sh ，轉染細胞，熒光顯微鏡觀察熒光定位； bfgf作為刺激因素處理細胞，根據不同濃度、時間檢測sh基因表達情況。
  9. Indirect immuno - fluorescent assay and enzyme linked immunosorbent assay ( elisa ) showed that the expressed protein was reactive with chicken anti - ibdv serum

    Dna疫苗對bc6 85 、 zj2000 、 zj991等3株強毒株具有良好的免疫保護效果，平均保護率達83
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