fluorescent cell 中文意思是什麼

fluorescent cell 解釋
熒光試池,熒光細胞
  • fluorescent : adj. 1. 熒光的，發熒光的。2. 外表華麗的；光輝四射的。3. 〈美口〉容光煥發的。n. 〈美口〉熒光燈。
  • cell : n 1 小室，單室；隔間，艙；〈詩〉茅舍；（單個的）蜂窩，蜂房。2 〈詩〉墓穴，墓。3 （大修道院附屬的...
  1. The progress of research on labeling of aberrant cell tissue by fluorescent probe or by connecting with biologically active carrier is reviewed

    簡述了熒光染料探針分子用於變異細胞組織的標記與識別的研究新進展。
  2. Materials and methods the mouse, golden hamster and human sperm were incubated with endotoxin in different concentration for different time to get capacitation, respectively, and ar was induced by progesterone after capacitation, then the rates of capacitation and ar were detected by chlortetracycline ( ctc ) and hoechst 33258 fluorescent staining method. the medium was with endotoxin in different concentration in sperm - oocyte fusion step during ivf, then the fertilization rate was observed. the 1 - cell, 2 - cell and zona - free 2 - cell mouse embryos were incubated in the medium with endotoxin, then the rate of blastocysts was recorded

    方法取小鼠精子10份、金黃地鼠精子6份、人新鮮精液標本10份及人冷凍精液標本9份，分別與不同濃度內毒素共孵育進行體外獲能和孕酮誘導的頂體反應，應用金黴素和dna結合的熒光染料hoechest33258雙重熒光染色法檢測精子的獲能率和頂體反應率；小鼠體外受精實驗的精卵結合環節培養液中加入不同濃度的內毒素，觀察受精情況並記錄受精率；取小鼠1 -細胞胚胎、 2 -細胞胚胎和去卵透明帶2 -細胞胚胎，與不同濃度內毒素共孵育進行體外培養，觀察體外發育情況並記錄囊胚率。
  3. Thus, in order to investigate the developmental pathways not only involved in the regulation of growth and patterning, but also in the determination of cell lineages and differentiation, we utilized the fluorescent immunohistochemical methods, flow cytometry analysis sorting ( facs ) and molecular methods to investigate the developmental law of mammary gland at the different developmental stages, distribution of the stem cells in mammary gland, the methods of isolation, culture and evaluation for the stem cells, the multipotent abilities in vivo and in vitro, and the efficient cultural system for stem cells enriched in vitro. the results showed below : 1

    我們以小鼠為模型，運用組織化學、免疫熒光組織（細胞）化學、流式細胞儀分選方法（ facs ）以及分子生物學手段，研究了小鼠乳腺的發育規律：小鼠乳腺組織中類乳腺幹細胞：小鼠乳腺細胞的分離、培養以及類乳腺幹細胞的鑒定；小鼠類乳腺幹細胞分化的潛能；小鼠乳腺類腺體體外短期培養富集類乳腺幹細胞體系的優化等。研究結果表明： 1
  4. Recombination gene was cut - down and introduced into the nuclei of oocytes or the cytoplasm of goldfish at one - cell stage via microinjection. the results as follows : ( 1 ) fluorescence was observed from embryo under suitable uv light after microinjection 36 hours. the fluorescent ratio of gastrula embryo period was up to 25 %

    採用顯微注射法將這種重組基因轉化1細胞期的金魚受精卵，實驗結果如下： （ 1 ）顯微注射后，根據胚胎發育分期，胚胎在顯微注射后36小時開始能在紫外燈下觀察到熒光，原腸期發熒光的胚胎比例為25 ，後期發育熒光率逐漸下降，肌肉效應期后又相對穩定。
  5. We used fission yeast schizosaccharomyces pombe ( s. pombe ), an unicellular eukaryotic organism, as research material. electroporation was adopted to load ca2 + fluorescent indicator into yeast cell and under the laser scanning confocal microscopy ( lscm ), we observed cytosolic ca2 + distribution and relative content as well as fluorescence intensity of gfp - cam in different phases of cell cycle of yeast cell. flow cytometry provided a way of determining the relative dna content of populations of fission yeast

    本文以單細胞的真核模式生物裂殖酵母（ schizosaccharomycespombe ）為研究材料，通過激光掃描共聚焦顯微鏡觀察酵母細胞胞質內游離ca ~ （ 2 + ）的分佈及相對濃度，以及不同周期時相細胞中gfp - cam的熒光強度變化，並採用細胞流式法對酵母細胞的相對dna含量進行測定以確定細胞所處周期時相。
  6. Labeling of three different mouse es cell lines with the green fluorescent protein

    在小鼠脊髓的表達和分佈研究
  7. Construction of recombinant adeno - associated virus vector expressing glial cell line - derived neurotrophic factor labeled by green fluorescent protein

    綠色熒光蛋白標記的大鼠膠質細胞源性神經營養因子重組腺病毒載體的構建及其表達
  8. The condition of profiles in outer station did n ' t change much in spring cruise, but showed more variable in near - shore stations when observed in different time. fluorescent characteristic per cell can be obtained by flowcytometric analysis. based on fluorescence data of synechococcus of all stations, two distinctly pigment - containing cell types coexisting can be found in some stations of east china sea, which located in all depth of p3, mixlayer of e7, 40 - meter depth of e6 of autumn cruise and in mixlayer of p2 of spring cruise

    通過對流式細胞計測量的細胞熒光結果來看，在秋季的p3 、 e7整個混合層、 e6站40米層，春季的p2站均發現有兩群不同色素含量的聚球藻（ high一pe和low一pe ）共存現象，極有可能分別屬于不同品系，春季共存站位位置比秋季時更靠外，表明在秋季p3 、 e7等站位的共存是季節性現象，可能與此季節黑潮次表層水沿陸架坡涌升入侵到中陸架有關，水團的運動及混合使從外海遷移而來的high一pe與近岸的low一pe得以共存，在春季，由於長江沖淡水的日漸強盛，在中陸架區的共存區域有所外移。
  9. Thereafter, the rfq - pcr method for the detection and enumeration of s. costatum cells is established with primer6 ( f / r ) and taqman6. the regression curve for enumeration is delineated according to the development of the fluorescent densities in the rfq - pcr with the increasing number of s. costatum cells. the regression equation is y = - 3. 3427x + 43. 443, in which x indicates the log10 of cell number, and y indicates the ct values, with r2 of 0. 9788

    以rfq一pcr中實際細胞數的常用對數值為橫座標，以測得的ct值為縱座標，繪制出了定量檢測的標準曲線，該曲線的回歸方程為: y =一3 . 3427x + 43 . 443 ，其相關系數是: rz二0 . 9788 ， ct值的標準估計誤差為sy 』 x二0 . 6741 。
  10. Measurement of cell calcium ( review ) methods for the intracellular ca2 + measurement such as aequorin, metallochromic indicators, ion - selective electrodes and fluorescent ca2 + indicators, particularly the fluorescent ca2 + indicators were reviewed in detail. 2. synthesis of new fluorescence ca2 + indicators and study on their properties tow new fluorescent ca2 + indicators stdin and stdbt have been designed and synthesized and their structures, spectral and biological properties have been studied thoroughly

    2 . 5一ht誘導胃底平滑肌細胞鈣火花的研究以合成的胞漿特異性caz +熒光探針stdhi一am標記細胞，採用激光共聚焦線掃描技術，首次觀察到5一ht誘導胃底平滑肌細胞胞內鈣釋放的最基本單位一一j塑必嘗華盆翔望嘗『一盈些沁（ elementaryevents ） ， 「鈣火花（ ealeiumsparks ） 」現象。
  11. Chapter two is the research results and discussion, which consist of distributions of cell density, fluorescent characteristic per cell of ultraphytoplankton. synechococcus and picoeukaryotes are abundant in all stations of east china sea and yellow sea, and prochlorococcus ca n ' t be found in near - shore stations

    第二章為在東、黃海所做工作的主要成果闡述，主要分析了由流式細胞計獲得的超微型浮游植物細胞密度、單細胞熒光在各站位的分佈特徵，結果如下：聚球藻（ synechococcussp
  12. In our research, marked autologous fluorescent blood red cell is immitted into sd - rat body and the whole process is shown and recorded by microscope image system. after these processes, we can replay the recorded tape and sample images with video image card. then, we use sequence image processing to analysis the image of dark ground microscope

    利用做過熒光標記的自身紅細胞注入sd大鼠體內，通過顯微圖象系統將整個過程以視頻信號的形式存貯，然後利用基於視頻圖象的採集卡，將流速變化過程回放采樣，得到暗視場下的熒光圖象，利用圖象分析和處理的方法，測定血流速度。
  13. After multiple, plasmid ploxifn and pbs185 dna were co - transfected into the green fluorescent cell clones, then filtrated the non - fluorescent cell clones. two non - fluorescent cell clones, the green fluorescent cell clones and the cells which were co - transfected by the plasmid ploxifn and pbsiss dna were checked up by pcr

    首先將質粒ploxgfpdna電轉染牛胎兒成纖維細胞，並用g418篩選，篩選出綠色熒光細胞克隆，增殖培養之後，將質粒ploxifn和pbs185dna共轉染熒光細胞克隆，篩選出不發光的克隆。
  14. Wang sp, grayston jt. serotyping of chlamydial trachomatis by indirect fluorescent - antibody staining of inclusions in cell culture with monoclonal antibodies [ j ]. j clin microbiology, 1991, 29 : 1295

    余加林，吳仕孝.連接酶鏈反應在沙眼衣原體感染診斷中的應用[ j ] .重慶醫科大學學報， 1996 ， 21 : 409
  15. The protein addresses the research need for a red - shifted fluorescent protein and will be an extremely useful tool for tracking and quantifying biological entities in the fields of biochemistry, biotechnology, molecular biology, cell biology and medical diagnosis complementing fluorescent proteins from other sources currently employed. professor wan said " it is gratifying to know that our discovery will be made widely available through stratagene s range of fluorescent protein products

    這種螢光蛋白不但有助於科學界深入對光譜紅移螢光蛋白的研究，而且在少數已知有輔助用途的螢光蛋白外，提供了一種能追蹤及量度生化物學的極為有用工具，能廣泛應用於生物化學、生物科技、分子生物學、細胞生物學和醫療診斷等范疇。
  16. This new method will bring significant developments in studying the principles of stomatal movement, and other quick movement in plants, c ) guard cells are incubated with ph dependent fluorescent chemical probe " bcecf am " and excited at 488nm, the fluorescent emission ratio method ( 520nm / 640nm ) is employed with laser scanning confocal microscopy, about 0. 4 ph unit increase in guard cell vacuoles is observed during stomatal closure that is induced by aba

    本發展為保衛細胞與其它小細胞液泡的進一步研究提供了新思路。 c ）本工作通過激光共聚焦顯微術配合ph熒光探針bcecfam的單激發（ 488nm ）雙發射的熒光比值法（ 525nm 640nm ）觀察到，用aba處理的表皮條上的開放態氣孔在關閉過程中其保衛細胞液泡內ph有一約0 . 4單位的上升。
  17. The structure characteristics of aberrant cell, identification marking, fluorescence labeling technologies and methods of cell labeling by fluorescent probe are recommended

    介紹了變異細胞的結構特徵、細胞識別與熒光標記技術以及熒光染料探針分子標記細胞的方式。
  18. The plasmid ploxgfp dna was transfected into bovine fetal fibroblast cells, then on the selected g418 medium, the green fluorescent cell clones were gained

    將雞-干擾素cdna克隆到載體plox上的loxp和lox511序列之間，構建成另一打靶載體? ploxifn 。
  19. After the examination of pcr, one non - fluorescent cell clone had the same result as the plasmid ploxifn - a 1000bp product ; another non - fluorescent cell clone showed a 500bp product, a deletion reaction was thought to happen between the two lox sites in the genome under the reaction of cre recombinase so that the gfp gene and neo gene etc were cut

    經pcr檢測后，有一個不發光細胞克隆擴增出了與對照質粒ploxifn相同的約1000bp的條帶，表明發生了替換反應；另一個不發光細胞克隆認為是在cre重組酶的作用下其內部自行發生了剪切作用，剪切了gfp基因，從而只擴增出了載體序列和一個lox位點的約500bp的條帶。
  20. Using the green fluorescent cell clones as the template, a product of 4kb which was the same as the result of pcr of the plasmid ploxgfp was got. on the other hand, not only a 4kb product but also a 1000bp product and a 500bp product were showed when the co - transfected cells were used to be the template

    以綠色熒光細胞克隆為模板擴增出了包括gfp基因、 neo基因等在內的約4kb的條帶；以共轉染后的未經篩選的細胞為模板既擴增出了約1000bp的條帶，又擴增出了約4kb和500bp的條帶。
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