fragement 中文意思是什麼
fragement
解釋
碎片塞-
In order to identifiy the virus further, a set of double nested primers for canine coronavirus was selected. the primers were designed in s gene region from ccv including two pairs of primers : ccvfl - ccvrl, ccvf2 - ccvr2. the first is a pair of outer primer, and can amplify a fragement of 1086bp. the second is a pair of inner primer. and can amplify a fragement of 515bp. using the nested primers, many ccv strains can be identificated including k378, insave - l, ccv 1 - 71 etc. synthesizing this set of primers, we selected the panda ' s liver - tissue materials and some different passages of viral culture to amplify by rt - pcr, and all of them respectively gained two target fragements of 1086bp and 515bp, but the control cell did not
合成該套式引物，選擇大熊貓原代病料和病毒各代細胞培養物，經套式（ nested ） rt一pcr擴增，可得到一與設計值5巧bp相符的dna片段，經bst一xl （ 590 ， 1110 ）酶切鑒定，證明該擴增片斷為特異性片段;回收大熊貓肝組織原代病料和細胞培養物第2 、 3 、 29代的ccvfz一ccvrz擴增片段，純化，送生物公司測序。 -
4 ) fragement recovery of pcr products pcr products were separated on a 1. 5 % agarose gel. the expected fragment was recovered by using pcr fragment recovery kit according to the manufacturers instructions
4 、凝膠回收應用pcr目的基因片段回收試劑盒對擴增陽性pcr產物進行凝膠回收，純化目的dna片段。 -
By rt - pcr method. we first selected a pair of primers named 2bp and 4bm which was designed in highly conserved 922 nucleotide region in open reading fram ( orf ) ib from coronavirus. this primer could amplify 11 kinds of coronaviruses. after synthesizing this pair of primers, we amplified the target fragement of 251bp from the panda ' s liver - tissue materials and other different passages of culture of this virus. however, the control cell showed negative result
第一對引物為外層引物，可擴增出1086bp的核酸片段；第二對引物為內層引物，可擴增出515bp的核酸片段。該引物以k378為參考株，含有多個兼并堿基，可擴增出包括k378 、 insavc - 1 、 ccv1 - 71 、 nvsl吉林農業大學碩士學位論文大熊貓犬冠狀病毒的分離鑒定及s墓因部分序列的測定和分析等多種犬冠狀病毒。 -
To construct eukaryotic expression vector of mbl gene with codon 54 point mutation, the target sequence in pgem - mbld plasmid, which conains mbl cdna with codon 54 mutant allele, was amplified by pcr. after the cdna fragement and plasmids pci - neo were prepared by digestion with sma i and sal i, the fragment was inserted into sma i and sal i site in pci - neo eukaryotic expression vector, and the recombinant vector, named pci - mbl54, was obtained. the pci - mbl54, digested with restriction enzymes, was found to contain the point mutation mbl cdna by agarose gel electrophoresis analysis
本實驗以ggc54gacmbl突變為研究對象，選用真核表達質粒pci - neo ，根據已構建好的含54位密碼子突變型mbl基因t載體的結構，設計合成新的引物， pcr擴增54位密碼突變型mbl基因，凝膠回收，雙酶切pcr產物和pci - neo質粒， t4連接酶連接，將前者克隆至後者的sma和sal位點，轉化大腸桿菌xl - 1blue ，氨芐選擇培養。
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