fragment chromosome 中文意思是什麼
fragment chromosome
解釋
斷片染色體- fragment : n. 1. 碎屑，碎片，破片，斷片。2. 未完稿，斷簡殘篇。vi. ,vt. （使）成碎片，（使）分裂。
- chromosome : n. 【生物學】染色體。
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Translocation occurs when a fragment of one chromosome becomes attached to a non-homologous chromosome.
一段染色體跟非同源染色體連在一起時就發生易位。 -
Other chromosome elements, telomere and ars, have also been cloned for constructing artificial chromosome. arabidopsis telomere was cloned from pcr products using telomere repeat primers without other template. a 2000bp fragment of ars was released from arabidopsis genomic bac clone t14a4 by claidigestion and subcloned into clai digested pbluescript
擬南芥的端粒是利用端粒的重復序列進行無模板的pcr擴增得到的；約2000bp的ars片段是從擬南芥的bac克隆t14a4中用限制性內切酶c1a1切下，然後亞克隆到通用載體pbluescript上。 -
The two end - points were localized on a 4. 3kb bamhl - ecor1 fragment of a cosmid 16c3 and a 1. 2kb sail fragment of a cosmid 17g7 respectively. the deletion junction was localized on a 2. 8kb bamhi fragment of the zx1 chromosome
兩個端點分別定位在野生型菌株基因組文庫粘粒16c3的4 . 3kb的bamh1 - ecor1片段上和17g7的1 . 2kb的sal片段上，缺失界點定位在zx1染色體上的一個2 . 8kbbamh片段上。 -
And the two pah ' s of pcr primers that bind to the adapter and the sequence of f fragment close by tn5 respectively were also designed. the genomic dna of b8 was isolated, digested with bamh i, and ligated to the adapter. using the two pairs of the primers, two rounds of pcr were performed hi turn and a fragment of 239bp was amplified successfully. lt was proved by cloning and sequencing that 18bp of the fragment is the sequence opposite to f fragment on the left of tn5 insertion site in b8f, the other is part of the 728 bp of f fragment. this result makes it possible to continue to carry out chromosome walking, to clone and sequence the whole genes of b fragment and f fragment, and to reveal the antagonistic molecular mechanism of b8
試驗研究設計併合成了由40和44個堿基的寡聚脫氧核苷酸組成的染色體爬行接頭，在接頭序列和測定的f片段近tn5的序列上，設計了2對染色體爬行用的pcr引物，從b8菌株中提取基因組dna ， bamhi酶切，與染色體爬行接頭連接，依次用2對引物進行pcr ，擴增出239bp產物，經克隆、測序，發現其中18bp為擴增的相應于f片段在b8f菌株tn5插入位點對面的序列，其餘則為f片段728bp序列的一部分，為進一步進行染色體爬行，克隆和測定整個b和f基因，揭示陽菌株的拮抗分子機制提供了技術資料貯備。
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