freund adjuvant 中文意思是什麼
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The western blot analysis show that the antiserum induced by the new antigen plus hemocyanin could bind with the 22kd and 55kd proteins, which were existed in the testis tissue protein of mouse, rat and human. 2 ) the purified peptide emulsified in an equal volume of freund ' s adjuvant and immunized the female balb / c mice with 8 - 10 weeks old. the antiserums and the washings of vaginal membrane were detected by elisa, and shown the highest level of the specific antibody igg was 1 : 6000, while the iga was 1 : 300
二、 p3多肽與弗氏佐劑混懸后免疫近交系balb c小鼠后，在血清和陰道粘膜沖洗液中可檢測出特異性lgg 、 lga ，最高效價分別達到1 ： 6000和1 ： 300 ；免疫后的小鼠脾臟淋巴細胞增殖率升高，淋巴細胞培養上清液中分泌的il 4和inf y也升高，且以il 4更明顯。 -
C57bl / 6 ( h - 2b background ) mice were subcutaneously immunized with selected peptides in ifa ( incomplete freund ' s adjuvant ) respectively. 8 days later after immunization, spleens were removed to prepare splenocytes suspension
將這些肽片段分別與不完全弗氏佐劑制備成乳濁液，皮下注射免疫c57bl / 6小鼠， 8天後，處死小鼠，取出脾臟制備脾細胞懸液。 -
Under the conditions of sds - page, it depolymerized into only one subunit of 28 kd, which reveal that the subunit of purified ferritin of pea is undivided 4. preparation of antiserum of pea ferritin the purified pea ferritin was mixed with freund ' s complete adjuvant or freund ' s incomplete adjuvant, and then administered through palmula, back, abdominal cavity, vena auricularis and other routes for rabbits. seven weeks later, the litre of antiserum reached 1 i 32
4 、免疫家兔制備豌豆鐵蛋白抗血清民二功尋咬徑b一侖一以純化的豌豆鐵蛋白作為抗原，並分別加入弗氏完全佐劑和弗氏不完全佐劑等兔疫家兔，經過足掌、背部皮下、腹腔和耳靜脈等途徑進行免疫， 7周后獲得效價高達1 ： 32以上的豌豆鐵蛋白抗血清。 -
6 hours later, the amount of the expression yield of cp accounting for the total protein of the cells was 28. 9 % ( about 2mg / ml cp ). after sds - page, the recovery of cp added the same volume of incomp lete freund adjuvant emulsified completely, injected rabbits by subcutaneous injection. every rabbit was injected 2ml emulsified antigen ( 300ug cp ) once a week
重組表達載體導入宿主菌e . colibl21 （ de3 ） ，用終濃度為1mmol / liptg誘導， 37培養6小時后， cp基因的表達量達到最高，每毫升菌液含目的蛋白的量約為2mg ； uvi光密度掃描分析表明cp基因的表達量占細菌總蛋白的28 . 9 。 -
The hbaf53 fragment obtained was used as antigens to immunize a rabbit. in the first immunization, equal volume of freund " s complete adjuvant was mixed with antigen solution to enhance the antigenicity
用獲得的抗原免疫實驗動物，首次免疫添加等體積的弗氏完全佐劑，以增強抗原的免疫原性。
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