gene amplification 中文意思是什麼

gene amplification 解釋
基因擴增
  • gene : n. 【生物學】基因。 dominant gene顯性基因。
  • amplification : n. 1. 擴大；擴充。2. 【電學】增幅，放大（率）。3. （聲明等的）補充材料。
  1. The detection of ribosomal rna gene amplification during mouse oogenesis

    基因擴增的測定
  2. Degenerate oligonucleotides to highly conserved regions of cucumis melo 1 - aminocyclopropane - 1 - carboxylic acid ( acc ) oxidase gene were used to prime the amplification of fragment of 128bp by ploymerase chain reaction ( pcr ) in samples of genomic dna from fruit of cucumis melo l. cv hetao flesh, which was cloned into plasmid vector pmd - 18 - t. the clon of antisense orientation were selected, and it was inserted downstream of camv35s promoter and enhancer " " of tmv into the plant expression vector pbinyxw, antisence expression vector pbinya was constructed. at the base that pollination and fertilization of cucumis melo l. cv hetao was studied, using pollen tube pathway transformate cucumis melo l. cv hetao, 76 fruit had been obtained, moreover, hardness and content of sugar were analysed

    本實驗以河套蜜瓜果肉基因組dna為模板，用甜瓜acc氧化酶基因特異寡核苷酸鏈為引物進行pcr擴增，得到128bp的擴增產物。將得到的擴增產物克隆到質粒載體pmd - 18 - t上，篩選反向克隆，然後將其反向構建到植物表達載體pbinyxw的camv35s啟動子和tmv增強子「 」的下游，構建成反義表達載體pbinya 。並在對河套蜜瓜授粉受精生物學研究的基礎上，通過花粉管通道法轉化河套蜜瓜，共獲76顆瓜，並進行了硬度和含糖量的分析。
  3. A double - strand cdna fragment of dh ( diapause hormone ) gene was obtained from kt - pcr amplification. the fragment was digested by two restriction enzyme nde 1 / ecor 1 and then was joined with pet - 30a ( + ) plasmid which was digested by nde 1 / ecor 1 too. then the outcome was transformed into escherichia coli bl21

    Pcr產物經nde / ecor雙酶切后，與同樣雙酶切的pet - 30a （ + ）質粒連接並轉化至大腸桿菌（ escherichiacoli ） bl21中，經檢測篩選，成功得到陽性克隆。
  4. Esterase gene amplification and mutation associated with insecticide resistance

    酯酶基因擴增及突變與昆蟲抗藥性
  5. Cluster analysis based on rapd showed that at 76 % similarity level, all tested isolated could be clusted into nine groups, i. e. 1 p. ostreatus and p. florida, ii p. ostreatm p. sapidus, p. spodoleucus and p. eryngii, iii p. colnmbinus, p. corlicalus, p. cornucopiae, p. nebrodensis and p. ferulae ; iv p. pulmonarius and p. sajor - caju, v three isolates with indefinite species, vi p. luber - regium, vii p. cilrinopileatus, viii p. djamor and p. salmoneoslramincus, ix p. abalonus and p. cysliodism. 4. a single uniform product 1. 46kb in size resulted from pcr amplification of the 5 " half of the 28s rrna gene for all isolates of pleurolus and the other three genera

    隨機擴增多態性dna的聚類分析表明，在76相似水平下，可將供試的側耳菌株聚成九大類，第一大類包括糙皮側耳、佛羅里達側耳；第二大類包括美味側耳、灰白側耳、剌芹側耳；第三大類包括哥倫比亞側耳、裂皮側耳、黃白側耳、阿魏蘑、白阿魏蘑；第四大類包括肺形側耳和鳳尾菇；第五大類為3株未定名的側耳；第六大類僅有具核側耳；第七大類為金頂側耳；第八大類包括紅平菇和桃紅側耳；第九大類包括鮑魚菇和囊蓋側耳。
  6. The materials as explant in transformation come from birch leaf, stem segment and leaf stalk, and the spider toxin gene was used as foreign gene for this transformation experiment. it showed that the best explant was the big leaf, on which the transformation frequency was 22 %. by gus detection, there were 43 percent of the plants with kanamycin resistance, and 100 percent of positive result, by pcr amplification, was gotten from random sampling

    利用雙元載體的根癌農桿菌lba4404菌株（ agrobacteriumtumefaciens ） ，含質粒pyhy （目的基因及npt 、 gus基因） ，對白樺試管苗莖段，葉柄，葉片三種外植體進行侵染，結果表明：大葉片生長勢強，為轉基因的最優外植體，轉化率能夠達到22 。
  7. Amplification of 18s rrna gene of babesia orientalis and study on its phylogenesis

    基因的擴增及系統發育研究
  8. Pcr amplification using 2 degenerate primers for nitrogenase fe protein gene was performed with chromosomal dna isolated from the 29 isolates. the result suggested that a nifh amplicon of 323 nucleotides was detected in 7 isolates and the 7 isolates are c4 c5 g1 g2, w5 t1 and t7. these pcr amplified fragments were cloned, and sequenced

    首先利用芽孢桿菌中芽孢的抗熱性將土壤溶液在100沸水中煮10 - 15分鐘，然後用選擇性無氮培養基進行初篩得到29株菌落形態不同的菌株；接著用固氮酶結構基因nifh的特異性引物對這29株菌進行pcr擴增，結果表明其中7個菌株具有nifh基因，這7個菌株的編號依次為： c4 、 c5 、 g1 、 g2 、 w5 、 t1和t7 。
  9. The association of larval and adult stages of ecologically important caddisfly ( insecta : trichoptera ) using mitochondrial dna sequences larval and adult amplification fragments were cloned and sequenced. dna sequences were aligned using clustal x software ( gene codes corporation )

    線粒休dna一coi 、 u基因序列在毛翅目成幼蟲聯系中的應用研究擴增並測定了4種鱗石蛾的成、幼蟲的mtdnacoi 、 coh及trna一leu基因序歹『 j 。
  10. Nineteen isolates among 89 isolates showed a lower resisance to nalidixic acid, ciprofloxacin, sarafloxacin, enrofloxacin, orbifloxacin and levofloxacin, and the remainder were susceptible. pcr was used for the amplification of intregron gene of all the salmonella isolates

    89株分離菌株中，發現有19株對萘啶酸、環丙沙星、恩諾沙星、沙拉沙星、奧比沙星、左氟沙星表現出輕度的耐藥性，其餘菌株為敏感菌株。
  11. The results showed about 490bp dna fragments were amplified. because the amplified products is specific to the p - subclass of the proteobacteria, the amplification of the amoa gene may be a powerful molecular tools for detecting and analyzing ammonia - oxidizing communities in environment

    由於基於此引物的擴增對proteobacteria -亞科氨氧化細菌具有特異性，所以amoa基因片段的特異擴增為我們檢測和鑒定環境樣品中氨氧化細菌的種群提供了一個有效的工具。
  12. Benzyl chloride were used for extracting genomic dna of aspergillus. niger 14, about 1. 5kb specific fragment was obtained from genomic dna of aspergillus. niger 14 by pcr amplification with primers ( forward primer5 " ataggcatcatgggcgtctct3 " reverse - primer5 " cagctaagcaaaacactccgc 3 designed according to the known sequences of the phytase gene in the gene bank and pyrobest ? dna polymerase, after ligated with pmd18 - tvector, transformated into e. colidh5a competent cell successfully. 3. nucleotide sequence analysis of the cloned fragment revealed the presence of the whole phya gene in pcr product

    用氯化芐法提取了aspergillus . niger14 ~ #基因組dna ，根據genebank中已知的黑麴黴植酸酶基因序列設計出一對特異性引物（上游引物： 5 ataggcatcatgggcgtctc3下游引物： 5 cagctaagcaaaacactccgc3 ） ，採用pyrobest ~ （ tm ） dnapolymerase （高保真dna聚合酶） ，通過pcr方法從aspergillus . niger14 ~ #基因組dna中擴增出了預期的1 . 5kb左右的特異性產物，將其與pmd18 - tvector連接后，轉化e . colidh5菌株的感受態細胞，經質粒抽提、酶切鑒定，確認該目的產物已得到成功克隆。
  13. 3. its shown from the results of dna digestion, hydantoinase gene amplification, rapd, eric - pcr etc, that the electrophoretic patterns of the products are obviously different form the original one

    分別提取的基因組后進行酶切分析、海因酶基因的擴增、 rapd 、 eric - pcr等檢測，兩者均呈現不同的電泳圖譜。
  14. The gpa1 gene was obtained via pcr amplification and was cloned in the two - hybrid dna binding domain vector pgbkt7 the combinant plasmid was designated as pgbkt7 - ga. - galactosidase assay indicated that got did not have the property of self - activation. after pgbkt7 - ga was transformed to yeast pj69 - 4a, we transformed arabidopsis vegetative tissue two - hybrid cdna library plasmids to yeast pj69 - 4a containing pgbkt7 - ga

    通過pcr擴增得到gpa1基因並將其克隆到雙雜交dna結合域載體pgbkt7中，得到的重組質粒命名為pgbkt7 - g ， ?半乳糖苷酶活性鑒定表明g不具有自激活特性，將其轉化到酵母菌pj69 - 4a中，再以此為受體菌轉化擬南芥綠色營養組織cdna文庫質粒。
  15. Liobagrus kingi and liobagrus marginatoides were designated as outgroups in the present study. the length of 16s rrna gene fragment obtained by pcr amplification ranges from 534 to 542 bp in the 11 sisorid species ( 31 samples ) investigated, and is 532 bp in l

    通過pcr技術，擴增了長度為532bp的金氏（魚央）和擬緣（魚央）以及長度為534 - 542bp的11種?科魚類（ 31個樣品）的16srrna基因片段。
  16. Experiment shows that tpsl gene can endow organism the ability of synthesis trehalose, the dephosphorylation of the trehalose - 6 - phosphate is not special, and it can be replaced by other phosphatases. the tpsl gene from saccharomyces cerevisiae was cloned by pcr amplification

    實驗證實tps1基因就可以使生物體獲得產生海藻糖的能力，酵母的海藻糖合成酶復合體中6 -磷酸-海藻糖的脫磷酸化作用是非特異性的，它可由生物體內的其它酯酶所代替。
  17. ( 2 ) deletions of four different lengths were conducted using pcr amplification with the information of sequence of pdf 1. 2 gene promoter. these mutated promoters were fused to the gus reporter gene and introduced into tobacco plants. the results of gus activity driven by these different constructs showed that the promoter sequence between positions - 300 and - 243 ( containing a gcc box and a g box - like sequence ) was an essential ja - responsive element region to activate the expression of gus reporter gene, and the - 300 bp position was defined as the boundary of the minimal functional promoter in response to ja signaling

    （ 2 ）對該啟動子的5 』端進行不同長度的缺失突變，突變的啟動子與gus構建的融合基因在煙草中受meja誘導的瞬時表達結果表明，該啟動子中- 300 - 243bp區域（有一個gccbox和一個gbox - likesequence ）是其應答meja處理所必須的區域，並將- 300bp作為該啟動子應答ja信號的最小區域的邊界位置。
  18. Part two : studies ; l. the sox gene of dinodon refozonatum was amplified by using a pair of primers which can amplify the conservative motif ( hmg - box ) of human sry gene. the amplification band was observed in both male and female dinodon refozonatum, whose length was consistent with that of human sry gene, which about 220bp. the result of sscp analysis showed that there were many differences in the sox gene sequence between dinodon refozonatum and human, and there was a few differences between male and female dinodon refozonatum. 2. using a pair of degenerate primers based on the conservative region, hmg - box, of human sry gene, six different fragments were amplified from either female or male dinodon refozonatum, then cloned by using pmd18 - t vector and sequenced

    結果顯示： （ 1 ）赤鏈蛇基因的擴增片段與人sry基因擴增片斷大小相同，為220bp左右； （ 2 ）雌、雄赤鏈蛇sox基因的擴增片段大小雖然與人的相同，但其單鏈遷移率與人的有較大差異，而且雌雄個體間有明顯差異，預示該基因的dna序列在雌雄個體中可能有差異； 2 、參照人sry基因hmg - box保守區的序列，又設計一對兼并引物，擴增了赤鏈蛇的sox基因，並對擴增產物進行了克隆和測序。
  19. Efficient amplification and sequence analysis of chalcone synthase gene from ginkgo biloba by thermal asymmetric interlaced pcr

    方法快速克隆銀杏查爾酮合成酶基因及序列分析
  20. Pcr methodology was adopted for cloning of chitinase encoding genes. based on the sequences of chitinase gene from genebank, the primers for chitinase gene amplification were designed. pcr fragment was ligated with pmd18 ~ t vector and transformed into e. coli dh5 a

    盡管同源性比較低，但酶活性檢測發現該基因片段具有幾丁質酶活性，認為此pcr片段含有幾丁質酶編碼基因的全序列或部分序列，此基因片段是一個新基因或基因片段。
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