gene pairs 中文意思是什麼

gene pairs 解釋
基因對
  • gene : n. 【生物學】基因。 dominant gene顯性基因。
  • pairs : 花樣滑冰雙人滑
  1. Two positive clones were sequenced, and the results showed that its nuclcotidc sequence includes an open reading segment which codes for a 45 - amino acids protein and three endonuclcase sites which arc1 bgii, bamh i and bgi ii, this protein was identified as metallothionein based on its characteristic described above and its similarity ( 85 % ) to the mtn gene of drosophila : the 10 cysteine residues present occur in five pairs of cys - x - cys, x is serine, valine, ilistidine or lysine

    結果顯示：擴增的cdna片段長度為289bp ，其中含有一個編碼45個氨基酸的開放閱讀框，閱讀框所編碼的氨基酸中含有10個半胱氨酸，且在序列中均排列成cys - x - cys ，其中x為ser 、 val 、 his或lys 。這些特徵說明擴增的基因片段為家蠅mt基因序列的一部分。此基因序列片段與果蠅mtn基因序列的同源性達到85 . 0 ，擴增的基因序列中含有三個內切酶位點bg 、 bam和bg ，這一點也和果蠅mtn基因十分相似。
  2. To study on structure and inheritance of rh d gene interaction between gene expression of rh d and rh c / e and influences on rh d gene expression of inserts and rh box - methods : 20 pairs of oligonucleotide specific primers for exon, intron 2 4, insert and rh box of rh d, rhc / e gene were designed and composed the polymerase chain reaction - sequence specific oligonucleotide primer ( pcr - ssp ) was used to amplify the rh c / e gene, rh d gene, exon, intron, insert and rh box in 106 samples of unrelated individuals and 7 han nationality ancestries and 5 wei nationality ancestries whose proband were rh d - negative

    目的：觀察中國漢族非血緣關系隨機個體、漢族及維吾爾族家系rh血型的c e基因、 d基因外顯子、內含子、插入片段以及rhbox ，研究rhd基因結構及遺傳規律， d基因表達與c e基因的關聯，以及插入片段和rhbox對d基因表達的影響。
  3. In this paper, a field strain of infectious bronchitis virus was isolated from proventriculus tissue, morphological observation by electron - microscope and the biological characterizations of the virus were studied, pairs of specific primers are designed and synthesized in correspondence with them, according to the published sequences of infectious bronchitis virus three structural protein ( spike protein s membrane protein m nucleocapsid protein n ) genes, the cdna of si gene, s2 gene, m gene. n gene of ib v isolate lx4 were amplified by rt - pcr and full sequences were first reported

    在此基礎上，根據國內外已發表的ibv基因序列，分別設計特異性引物，應用不同引物進行反轉錄合成cdna ，分片段對ibv的主要結構基因進行pcr擴增，並分別將各個目的片段克隆到puc19載體上，在大腸桿菌dh5中實現目的基因的分子克隆，經藍白斑篩選、限制性內切酶分析、 pcr鑒定，篩選出重組陽性質粒，並對各個目的基因片段進行序列測定，從而獲得ibv主要結構基因全序列。
  4. 7. at the first time, the reporter dye, fam was linked to the 5 " - end of the oligonucleotides of the probes, and the tamra was located at the 3 " - end as quencher dye. we use camv35s and fmv promoter, nos terminater, mark gene nptii, and aim gene pat, epsps and cryla ( b ) genes as target sequences, design pairs of sp

    7 、首次以fam熒光素標記探針5 』端作為發光基團，以tarma標記探針3 』端為淬滅基團，以camv35s 、 fmv啟動子、 nos終止子、標記基因nptll 、抗除草劑基因epsps 、 pat 、抗蟲基因cry1a ）為檢狽目標，設計、篩選出特異性引物和探針，優化實驗參數，建立了轉基因植物通用性熒光pcr定性檢測方法體系。
  5. In this paper, total dna from tannage, tail skin, scales and the skin treated with salt were successfully extracted with an improved method for dna extraction. to verify the results, four pairs of primers, which were universal primers for 12s rrna gene, diagnostic primers of chinese alligator meat, microsatellite primers and rapd primer, were used to do pcr amplifications. some amplified fragments were sequenced, either

    本文研究出一種改進的dna提取方法，成功地從揚子鱷鞣製皮革中提取了總dna ，同時對不同的組織標本如鱗片、鹽腌生皮及尾尖皮等進行了dna提取：並利用12srrna基因擴增的通用引物、揚子鱷鑒定性引物、微衛星引物及rapd引物進行pcr擴增，且對部分pcr擴增結果進行測序，以檢驗提取效果。
  6. We designed one primer pairs ctb - 1, ctb - 2 to amplify about 580bp of cyt b gene sequence as a molecular marker to analyze phylogenetic relationship of 14 species of oedipodidae in china. dna sequences were aligned using clustal x, followed by refinement by eye based on the corresponding deduced amino acid sequences. after cutting off 5 " and 3 " termini unaligned sequences, we get 462 bp segment

    本研究從分子生物學角度入手，採用cytb作為分子標記，採用自行設計的一對cytb基因特異引物ctb - 1 、 ctb - 2 ，通過pcr技術，共獲得斑翅蝗科4個亞科14個種的代表個體以及癩蝗科1個種的代表個體的580bp左右的cytb部分序列。
  7. In order to identifiy the virus further, a set of double nested primers for canine coronavirus was selected. the primers were designed in s gene region from ccv including two pairs of primers : ccvfl - ccvrl, ccvf2 - ccvr2. the first is a pair of outer primer, and can amplify a fragement of 1086bp. the second is a pair of inner primer. and can amplify a fragement of 515bp. using the nested primers, many ccv strains can be identificated including k378, insave - l, ccv 1 - 71 etc. synthesizing this set of primers, we selected the panda ' s liver - tissue materials and some different passages of viral culture to amplify by rt - pcr, and all of them respectively gained two target fragements of 1086bp and 515bp, but the control cell did not

    合成該套式引物，選擇大熊貓原代病料和病毒各代細胞培養物，經套式（ nested ） rt一pcr擴增，可得到一與設計值5巧bp相符的dna片段，經bst一xl （ 590 ， 1110 ）酶切鑒定，證明該擴增片斷為特異性片段;回收大熊貓肝組織原代病料和細胞培養物第2 、 3 、 29代的ccvfz一ccvrz擴增片段，純化，送生物公司測序。
  8. Further sequence analysis show that only 6 base pairs of nucleotide and 2 amino acids are different between them. the homological cry3aa gene was expressed in escherichia coli. and the expressed products which contain a fused peptide of 66 - 97 kilo - dalton was observed by means of sds - page

    生物活性測定結果表明該菌株對榆藍葉甲（ pyrrhaltaaenescens （ fairmaire ） ）和光肩星天牛等鞘翅目昆蟲具有較高的毒力，因此初步確認該菌株屬于cry3類； （ 2 ）發現該菌株中編碼毒蛋白的基因位於質粒上，並且已經成功地克隆到該基因。
  9. This thesis studied on bacillus thuringiensis strain bt886 which was separated and selected by researchers of our laboratory. according to the observation of crystal shape and the bioassay of motschulsky and fairmaire, bacillus thuringiensis strain bt886 was identified as cry3 type, and the conclusion was assured by the further study on molecular level. the 1956 base pairs full lengrh homological cry3aa gene which was toxic to motschulsky was cloned and sequenced

    以該菌株為材料，克隆出了對光肩星天牛（ anoplophralabripennis （ motsch . ） ）具有毒殺作用的cry3aa同源基因，並且對該基因進行了人工改造、人工合成、大腸桿菌表達、生物活性測定、雙元表達載體的構建以及對楊樹的轉化等一系列研究，主要結果如下： （ 1 ）顯微觀察該菌株所形成的伴孢晶體為方形。
  10. Primers of ecori were signed with fluorescent dye and four pairs of primers were selected from twenty - four pairs. 261 dna fragments were generated by the four pairs of primers in forty - three individuals sampled. percentage of polymorphic bands is 51 %, the value of shannon index ( i ) is 0. 086 and nei ' s gene diversity ( h ) is 0. 151

    統計這些aflp表型帶標記，用popgene軟體計算各種多樣性數據（ ppb值為51 ， shannon多樣性指數為0 . 086 ， nei ' s基因多樣性值為0 . 151 ） ：用ntsys軟體計算各個樣品間的相似性系數，並用upgma法基於相似性系數進行聚類分析，構建樹狀圖，它們可反映出元寶山冷杉種群內個體間的遺傳關系。
  11. For these goals, the fo llowing jobs have been done and some results have been obtained. 1 according to the vitreoscilla hemoglobin ( vhb ) amino acid sequence and plajnt preference codon usage, vgbm gene was designed and synthesized by annealing 22 synthetic fragments respectively, the modified vgbm gene of full length of 450 base pairs was synthesized. 2 the transformants were obtained after pbv221svhb was introduced into e. coli

    為此本論文作了以下工作並得出了一些結論： 1根據透明顫菌血紅蛋白（ vhb ）的氨基酸序列，選用植物偏愛密碼子，對透明顫菌血紅蛋白基因（ vgb ）進行優化改造，設計併合成了22條寡核苷酸短片段，人工合成了改造的全長450bp的透明顫菌血紅蛋白（ vhb ）基因（ vgbm
  12. Three pairs of primers were designed according to the sequences pulished by the genebank in order to amplifiy gd, ge and tk gene of the pseudorabies virus min - a strain. the gd, ge and tk gene were obtained by polymerase chain reaction ( pcr ), and then cloned into the pgem - t easy vector

    參考genebank收錄的偽狂犬病病毒gd 、 ge 、 tk基因的序列設計了三對引物，對prvmin - a株進行了pcr擴增，擴增產物克隆于pgem - teasy載體。
  13. The mechanism is that the introduced complementary oligonucleotides can bind to the corresponding mrna or double - stranded dna in genome and form partial double - stranded molecules or triple - stranded nucleic acid molecules by sequence - specific and nonsequence - specific antisense action, thus the target gene will be orientationally blocked and expression of the target inhibited so that therapeutic effect could be attained. in this study, we designed a fragment of human c ii ta cdna in antisense orientation using mrna of c ii ta as template. the primers were designed based on 94 - 500 nucleotides segment in 5 " end of ciita gene so that the interested gene contained 407 base pairs which included two aug codons in 1 16 and 188 nucleotides as well as the splicing site between the first and the second exons

    本研究設計以c tamrna為模板的反義cdna片段，從c ta基因5 』端第94位到500位核苷酸段設計引物，目的片段407bp ，覆蓋第116和188位兩個aug密碼子，也包含了第一外顯子和第二外顯子間的剪接位點：用常規分子生物學方法構建了反義片段的腺病毒表達載體（ padeasy - 1系統） ；腺病毒載體經hek293細胞包裝產生含反義片段的重組腺病毒，用氯化銫密度梯度離心法獲得純化的高滴度腺病毒；進行體外基因轉移，分別用反義片段真核表達載體轉染p388d1細胞和用重組腺病毒感染hela細胞，觀察導入的c ta基因反義rna抑制細胞內組成型或誘導型c ta基因表達的作用，從而達到調控mhc -類分子表達的目的。
  14. In abroad, the study of integration site used for transgenic detection had just begun. in this study, according to the collection of the global commercialized transgenic crops, select seven exogenous genes which basically cover the total commercialized crops, namely camv35s and fmv promoter, nos terminater, mark gene nptii, and aim genes pat, epsps and cryia ( b ). use endogenous 18srrna gene as collate, design a large pairs of specific primers, screen the optimum primers groups, optimized the test condition and parameters, establishing the qualitative pcr detection system

    本研究根據收集的國內外已商品化的轉基因作物品種，選擇了能基本覆蓋商品化轉基因品種的7個外源基因，即： camv35s 、 fmv啟動子、 nos終止子、 npt標記基因和目的基因pat 、 epsps 、 cryia （ b ）作為篩選目標，以植物18srrna基因作為內源參照基因，設計了多對特異性引物，並篩選出最佳組合，優化了檢測條件和參數，建立了pcr定性檢測方法體系。
  15. Some gene mutations are simply alterations of one base pair among the total three billion base pairs of dna in the human gnome

    許多基因變異是由於人類總基因為數三十億的鹼基里其中一對鹼基出了問題。
  16. Sequencing of the whole alpha - amylase gene of bombyx mandarina ( chongqing ) based on the published nucleotide sequence of alpha - amylase gene of bombyx mori, seven pairs of primers were designed for the sectional amplification and cloning of the alpha - amylase gene of bombyx mandarina. the combined sequence, i. e. the whole alpha - amylase gene of bombyx mandarina, is 8903bp in length

    重慶野桑蠶-澱粉酶基因全序列的測定根據家蠶-澱粉酶全基因序列設計了的7對引物，對野桑蠶-澱粉酶基因進行了分段擴增，並逐一克隆測序和序列拼接，得到8093bp的野桑蠶-澱粉酶基因全序列。
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