gene replacement 中文意思是什麼

gene replacement 解釋
基因替代
  • gene : n. 【生物學】基因。 dominant gene顯性基因。
  1. In order to facilitate the study of biological function of add gene cluster, e. coli - s. avermitilis intragenus conjugation system was established. in addition, phz2114 for the replacement of the entire add gene cluster and phz2130 for disruption of adda were constructed

    建立了除蟲鏈黴菌的接合轉移系統，並構建了用於置換全部基因簇的基因置換質粒phz2114和adda的基因中斷質粒phz2130 ，為研究除蟲鏈黴菌add基因簇的生物學功能奠定了基礎。
  2. Shaking flask experiments and hplc analyses showed that 99 isolates still produced four components of avermectins b, in which the yields of avermectins b in 82 isolates were only about 0. 1 ~ 2 % of those produced by the parental strain olm73 - 12. 2 of 82 isolates were confirmed to be the correct gene replacement mutants by pcr and sequence analysis. the mutant only producing avermectins bl was not detected

    搖瓶發酵和hplc分析結果表明，有99個突變株仍然產阿維菌素b的4個組分，其中82株的發酵單位很低，僅為出發菌株olm73 - 12的0 . 1 2 ，從中挑取2株經pcr擴增和測序驗證，均發生了正確的基因取代；沒有檢測到僅產b1的突變株，這表明阿維菌素b2組分的產生並不是因為阿維菌素pks上dh2的部分活性所造成。
  3. To clone large and random dna fragments, the second generation of yac, pjs97 - pjs98 was modified as gene replacement vector in streptomyces

    4kb和1 . okb片段，而且還插入了勿，七刃j和spc心t廠抗性基因。
  4. Such therapy - - the actual correction or replacement of defective gene sequences in the embryo or the adult - - is the holy grail of genetic medicine

    這樣的治療法一對胚胎中或成人體內的缺陷性基因序列進行切實可行的修正或替換? ?是遺傳醫學的聖杯。
  5. Thirdly, the hyl gene was cloned to puc19 and pbr322 respectively. then the fragment containing ampr derived from puc19 and hasa came from pse380 - has were inserted into hyl either or both in vitro. transformed the recombinated vectors into s. equi by electro - operation, then plating on hag solid medium containing ampicillin, and selected clones hi which the hyl gene was inactivated by gene replacement through homologous recombination

    （ 1 ）直接插入外源基因滅活hyl方法：將hyl片段與載體puc19 pbr322連接后，用來自puc19的amp ~ r抗性基因片段從hyl基因的中部插入將載體上的hyl片段分為兩部分； （ 2 ）用hasa替換hyl部分片段的方法：將hyl片段與載體pucl9 pbr322連接后，用hasa片段替換部分hyl ，再將amp ~ r片段接入到hasa上游。
  6. For desired gene replacement, hyg / cml and str / spc gene cassettes were also incorporated into the new vector system. it is expected that hygwould be integ

    為了驗證新型yac載體系統的功能，我們將phz621 ~ phz622進行了適當地改造，構建了質粒phz625 。
  7. In this study, we attempted to construct an engineering strain producing only avermectin bl through the replacement of dna encoding dh2 - kr2 domains of the avermectin pks ( avedh2 - kr2 ) with dna encoding dh2 - kr2 domains from the pikromycin pks in s. avermitilis olm73 - 12, producing only avermectins b and no oligomycin. gene replacement vector pxl201 ( pkc1139 : : 5 ' flank + pia : dh2 - kr2 + 3 ' flank ) was used to transform 5. avermitilis olm73 - 12 protoplasts

    我們以不產寡黴素而僅產阿維菌素b的工程菌olm73 - 12為出發菌株，用委內瑞拉鏈黴菌（ streptomycesvenezuelae ）中編碼pikromycinpks模塊2上完全活性的dh和酮基還原酶（ kr ）的dna區域對olm73 - 12染色體上編碼阿維菌素pks模塊2中dh和kr的區域進行取代，試圖構建僅產b1組分的基因工程菌。
  8. Streptomyces low copy number plasmid scp2 * is also employed for the cloning and recovery of large dna fragment from streptomyces. as scp2 * can accommodate large dna insertions and is efficiently transmissible among its permissive hosts such as s. lividansbut seemed to be unable to replicates in streptomyces sp. fr - 008, it might be a suitable vector for the cloning of the entire fr - 008 pks gene cluster through gene replacement followed by conjugation with 5

    Scp2 *可以在變鉛青鏈黴菌等許可宿主內復制並在許可宿主間有效轉移，但似乎不能夠在鏈黴菌fr - 008中復制，因此可能適合於在鏈黴菌fr - 008中通過進行基因置換及隨后的鏈黴菌fr - 008和變鉛青鏈黴菌間的接合過程來克隆完整的fr - 008生物合成基因簇。
  9. To facilitate the detection of gene replacement, apr gene was placed in the middle of the two inserts. orit from e. coli plasmid rk2 was also incoporated into the vector, therefore, pij903 derivatives can be mobilized from e. coli into streptomyces sp. fr - 008 by either bi - parental conjugation or by conjugation between streptomyces

    為了在克隆完整基因簇時避免這兩方面的影響並能隨機地獲得dna大片段，我們將第二代yac載體pjs97 - pjs98改造成為適合於在白林泉鏈黴菌基因組中dna大片段的基因置換與克隆鏈黴菌中分析被克隆大片段功能的基因置換載體。
  10. The complicated life cycle of streptomyccs is one of the most important reason that attracts researchers on its genetics. a great deal of valuable information on the morphological, physiological differentiation of streptomyccs coclicolor will emerge after genome sequencing is completed. based on the s. coelicolor dna sequences that have been finished and focusing on three ftsk homologous, which may be closely related with cell division, this study tried to find out the functions of three ftsk homologues genes by gene replacement or disruption

    鏈黴菌復雜的生活史是研究其遺傳學的一個重要原因，天藍色鏈黴菌全基因組測序將會為其形態及生理分化發育的研究提供大量有用的信息，本研究基於天藍色鏈黴菌已測出的98序列，對一組可能與細胞分裂有關的基因? ftdk同源基因? ?進行基因置換或中斷並對其功能進行初步研究。
  11. Secondly another vector phz1117 which for replacement of sc ( j11. 17 / scd78. 01, was constructed. phz1117 was transformed into wild type strain m145 and a gene replacement strain named hxy3 was obtained

    其次，構建了另一個用於置換scq11 . 17 scd78 . 01的置換載體phz1117 ，得到基因置換菌株hxy3並通過southern雜交驗證，與野生型m145相比，沒有觀察到hxy3有明顯的表型變化。
  12. Pfge analysis of these 21 blocked strains revealed a common chromosomal deletion in the 300kb asel fragment which might be responsible for antibiotic 5102 - iii biosynthesis. so the 300kb fragment was recovered and used as probe to hybridize with 10 - 22 genomic library. cosmids in this region were aligned and suitable fragments in this region were selected and used further for the construction of gene replacement plasmids

    以此片段為探針釣出了位於該區域的文庫克隆，並利用指紋印跡和雜交技術將這些文庫克隆排列起來，進而以位於該區域的不同位置的片段做臂構建了用於轉化和接合轉移用的基因置換質粒，並試圖通過基因置換將該區域置換下來，但尚未得到最終結果。
  13. Such deficiencies cause lysosomal storage disorders, such as gaucher ' s disease, and replacement of the gene is an effective treatment

    這樣的缺陷引起溶酶體堆積病（例如，高歇病） ，基因置換是一種有效的療法。
  14. Phz621 - phz622, the new gene replacement vector system, had been constructed through the insertion of the 1. 0kb and 1. 4kb flanking sequences of hau3r gene from wild - type s. lividans in the same natural orientation

    預期在攜帶有大插入片段的基因置換yac分子進入野生型變鉛青鏈黴菌后， yac分子將通過1 . 4kb或1
  15. Lividans mutant strains la1 and la2 generated by gene replacement in dndb and dndc were obtained respectively. both of these two mutants lost dnd phenotype because of disruption of a respective dnd gene

    首先利用基因置換實驗獲得分別在dndb 、 dndc基因內部發生基因中斷的突變菌株la1利la2 ，並通過southern雜交進行了驗證。
  16. 2kb or 7. 2kb regions abolished the productivity of antibiotic fr - 008. the expected strain after gene replacement in the region of 5. 2kb have not been obtained for unknown reason

    盡管由於未知的原因尚未獲得5 . 2kb區域的基因置換菌株，但是以上的試驗證明基因置換過程是可以在鏈黴菌fr - 008中進行的。
  17. Phz1114 was transformed into the m145 and a gene replacement strain named hxy2 was obtained and testified by southern blotting

    在mmasd及sfm上培養時發現： hxy2子代菌落有表型分離現象。
  18. Prior to the vector construction, the availability of gene replacement was extensively tested in streptomyces. sp. fr - 008

    在載體構建之前，我們先在鏈黴菌fr - 008中驗證了基因置換方法的可行性。
  19. All the subjects were genotyped by pcr - rflp ( polymerase chain reaction - restriction fragment length polymorphism ) at polymorphic sac i site inside the exon 7 of the ahsg gene. this polymorphism involves a nucleotide substitution of c to g at the middle nucleotide of the codon at amino acid position 238 resulting in the replacement of threonine ( acc ) with serine ( agc )

    所有的樣本通過聚合酶鏈式反應?限制性片段長度多態性方法（ pcr - rflp ）對ahsg基因的第7個外顯子內的sac多態性位點進行基因分型，該多態性位點為238號氨基酸密碼子中間的堿基c到g的替換，使蘇氨酸（ thr ， acc ）變為絲氨酸（ ser ， agc ） 。
  20. The total dna of exoconjugants acquired dna degradation phenotype during electrophoresis, which suggested that the dnd gene cluster was heterologously expressed. the phz1358 was used sucessfully as a vector for gene replacement to identify the biosynthetic pathway genes for nanchangmycin in s. nanchangensis ns3226. however, the gene replacement in another potential pks cluster has not succeeded till now

    將利用phz1358構建的基因置換結構（孫宇暉，未發表）導入南昌鏈黴菌ns3226中進行了基因置換實驗，協助完成了南昌黴素生物合成基因簇的克隆，但對另一潛在pks基因簇的基因置換實驗目前還未能完成。
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