gene-cloning vector 中文意思是什麼
gene-cloning vector
解釋
基因無性繁殖系載體-
To test the p7 promoter activity, a series of constructs were obtained by cloning the different dna fragments into the luciferase gene reporter vector pgl3 - b. when the constructs were transiently transfected into thp - 1 cells, luciferase activity assay showed that the core region of p7 promoter located at - - 165 bp
第一部分的工作首先通過對人acat - 1基因p7啟動子序列進行5 '和3 ' -端的缺失的詳細分析，結果顯示最大報告基因轉錄表達活性位於- 612 - 165bp區段。 -
Cloning of monomial promoter of actin1 gene and construction of plant expression vector
1的克隆及植物表達載體的構建 -
Cloning and construction of prokaryotic expression vector of vp1 gene of foot - and - mouth disease virus serotype o
1基因的克隆及原核表達載體構建 -
Part 1 : identification of a novel gene, tsarg2, and its sequence character cloning new apoptosis - related novel gene is a key to further understanding of apoptosis mechanism and the biological process of germ cell, and it is of momentous significance on clarifying physiology and pathology process of spermatogenesis. to rapidly attain human novel gene full - length cdna sequence, the gene - specific primers and the vector - specific primers have been designed for successful performing nested pcr and draft human genome searching to rapidly identify the tsarg2 ( genebank accession number ay040204 ) 5 " end from a human testis cdna library by using a cdna fragment ( genebank accession number be644542 ) as a electronic probe, which was significantly changed in cryptorchidism and represents a novel gene. furthermore, a mouse homologue of this gene was identified ( genebank accession number af395083 ) by lab on - line
本研究分為三個部分，其主要實驗方法及實驗結果如下：第一章tsarg2基因的克隆與序列分析從已獲得的在隱睪和正常睪丸對照中表達量有明顯差異的est片段（ be644542 ）入手，設計了基因特異性引物和載體特異性引物進行巢式pcr擴增，結合人類基因組草圖搜索法，從睪丸cdna文庫中快速分離出人類睪丸凋亡相關基因的5末端而獲得全長cdna ， genbank登錄號為ay040204 ，同時應用生物信息學的方法克隆了該基因在小鼠中的同源基因， genbank登錄號為af395083 。 -
Firstly, the hyaluronate synthase ( has ) gene ( hasa ) was cloned into the cloning vector puc19 by pcr using the total dna sample of s. equi as template
本文研究代謝工程理論，對本實驗室的一株ha生產菌（ streptococcusequi ）進行了ha合成與分解關鍵酶基因及功能的研究。 -
The hwtx - i gene was chemically synthesized according to its known cdna sequence, the gene was inserted into vector ppic9k which contained aoxj promotor and the sequence of a secreting signal peptide - a - factor, the cloning ppic9k / hwtx - i was constructed and confirmed by two - step pcr and dna sequence analysis, then it was transformed into host strain gs115, a his + muts cell line was screened and multicopy transformants were screened by various g418 concentrations, the multicopy transformant was named gh1. gh1 was cultivated in flasks. after 6 days of induction by 0. 5 % methanol, the supernatant was checked by 16. 5 % tricine - sds page, which showed there was a band in the position of 3. 5 - 6. 1kd, then it was isolated and desalted by ultrofiltration followed by ion exchange of cm column, after reverse phase hplc of ci8 and vacuum drying, the purified rhwtx - 1 was obtained which was proved to be correct recombinant hwtx - i by tricine sds - page, maldi - tof mass spectrometry, amino acid composition analysis, the n - terminal amino acid sequence and its biological activity, the final field of the purified rhwtx - i was about 80mg / l, accounting for 23. 6 % of it total secretory proteins
將帶有hwtx -基因的ppic9k經blgii線性化后，轉化酵母宿主菌gs115原生質體后經篩選陽性克隆並經表型鑒定為his ~ + mut ~ s酵母菌，進一步用遺傳毒素g418篩選多拷貝的轉化菌株，命名為gh1 ；將gh1甲醇酵母菌用0 . 5的甲醇誘導表達，發酵上清經90飽和度的（ nh _ 4 ） _ 2so _ 4沉澱， yw - 3 （ mwc03000 ）的超濾膜超濾，再經cm陽離子交換， c _ （ 18 ）反相hplc純化得到分子量為4kd左右的組分，其中4289 . 05的組分經質譜鑒定，氨基酸組成分析和序列測定為正確的表達產物，生物學活性表明其活性為天然毒素活性70 % ，表達量為80mg / l 。 -
Cloning of rejection related gene and construction of antisense gene transferring vector
玉米溫敏核雄性不育相關基因克隆 -
Streptomyces low copy number plasmid scp2 * is also employed for the cloning and recovery of large dna fragment from streptomyces. as scp2 * can accommodate large dna insertions and is efficiently transmissible among its permissive hosts such as s. lividansbut seemed to be unable to replicates in streptomyces sp. fr - 008, it might be a suitable vector for the cloning of the entire fr - 008 pks gene cluster through gene replacement followed by conjugation with 5
Scp2 *可以在變鉛青鏈黴菌等許可宿主內復制並在許可宿主間有效轉移，但似乎不能夠在鏈黴菌fr - 008中復制，因此可能適合於在鏈黴菌fr - 008中通過進行基因置換及隨后的鏈黴菌fr - 008和變鉛青鏈黴菌間的接合過程來克隆完整的fr - 008生物合成基因簇。 -
Cloning of egf gene from mouse kidney and construction of its plant expression vector
基因的克隆及其植物表達載體的構建 -
Cloning of a carrot gene encoding antifreeze protein and construction of its plant expression vector
胡蘿卜抗凍蛋白基因克隆及植物表達載體構建 -
( 3 ) cloning of mdmv - cp gene and construction of expression vector mdmv - cp gene was cloned from infectious maize leaf
對t1代、 t2代的表型鑒定及進一步的分子鑒定正在進行之中。 -
In this experiment, ri gene was amplified by pcr method from recombinant cloning vector pt7 - ri, which had been constructed by dr. yu xiu - ping in this laboratory and sequenced by takara biotechnology ( dalian ) co., ltd.
于秀平博士已經構建了ri的克隆載體pt7 - ri ，測序表明與已知人胎盤ri基因的cdna序列完全相同。本文的工作是在此基礎之上完成的。 -
When compared with kds301 ( lexa + ), xe ( lexa - ) cells showed quite similar levels of survival curve to y - irradiation and mmc, indicating lexa gene had no effect on radiation resistance of deinococcus radiodurans. deinococcus radiodurans " lexa had no revelance with its extremely radioresistance. the recombinant plasmid pza172 was constructed by cloning the lexa gene of deinococcus radiodurans into the vector plasmid puc19 in the downstream of lacz promoter
對lexa基因突變體xe的研究表明，其輻射抗性與lexa基因野生型的抗輻射菌kd8301相近，存活曲線上均顯示有一個寬大的肩區，結果發現lexa基因的突變並沒有改變抗輻射菌的獨特抗性，說明lexa基因與輻射抗性無關。 -
Two types of repeat sequence, a 15 - amino - acid ( eelcaqlcstppppi ) with 2 repeats and a 6 - amino - acid ( ppictp ) with 4 repeats, were firstly reported. 2. the characterization of meq gene product and its expression within the cells a recombinant baculovirus transfer vector pblubac4 - meq was constructed by cloning meq gene of marek ' s disease virus ( mdv ) ga strain into the baculovirus transfer vector pbluebac4 under the polyhedrin promoter
此外，研究還發現了meq基因的兩類有趣的重復結構，其中一類是含15個氨基酸殘基（ eelcaqlcstppppi ）的結構，有2個重復，另一類是含6個氨基酸殘基（ ppictp ）的結構，共有4個重復，它們全部分佈在meq蛋白c -端的轉錄激活域內。 -
Then the amplified mtb8. 4 and ms gene were subcloned into the unique nhe i and mlu i cloning sites of pci - neo expression vector
重組質粒pm 、 pms轉染ps15細胞，進行穩定表達， g418篩選抗性克隆后，用rl 』 - pcr檢測目的基因在mrna水平的表達。 -
First, a pair of pcr primers was designed to isolate fmdv - vp1 gene according to the published fmdv - vp1 sequence. after pcr of dna isolated from the tissues, the fmdv - vp1 gene was cloned into cloning vector. positive clones were analysed with restriction enzyme digestions and further identified with sequence analysis
首先，根據已知的fmdv基因序列，設計特異性引物，擴增出fmdv - vp1基因，將克隆到的vp1基因連接到測序載體上後用dna測序儀對該序列進行測序。 -
In order to further investigate the role of axudl in human tumor carcinogenesis and the potential association between the axudl gene expression status and the stimulation of transforming growth factor beta in human cancers, the present study was performed in three aspects as follows : ( 1 ) cloning full length enconding region cdna of axudl and construction of eukaryotic vector that expression the fusion protein of axud1 and influenza virus hemagglutin ha epitope tag ; ( 2 ) exploring the time and dose effects of tgf - 1 on the expression - of axudl gene in hepg2 hepatoma cells and spc - a1 lung carcinomas cells, and studying the effects of overexpression of axud1 on the expression of cell cycle and apoptosis related protein in hepg2 hepatoma cells ; ( 3 ) construction and expression of human axudl in e. coli m15. the following main results and conclusions can be obtained from the present study : 1. the full length ecnoding region of human axudl cdna from human peripheral blood lymphocytes was successfully cloned using one step rt - pcr method, and constructed into a eukaryotic expression vector which can be expressed a ha - axud1 fusion protein with axud1 and influenza virus hemagglutin ha epitope tag. the recombinant plasmid was identified by polymerase chain reaction, restriction endonuclease maping and sequencing, this expression vector might be instrumental to further study the function of axud1 protein in tumor cells
為了進一步研究axud1在人類腫瘤發生中的作用及axud1基因的表達狀況與tgf -介導的信號通路的關系，本實驗研究分為三個部分： （ 1 ） axud1基因cdna全長編碼區的克隆和ha表位標記的axud1基因表達載體的構建； （ 2 ）探討肝癌細胞hepg2和肺腺癌spc - a1細胞中tgf - 1誘導的axud1基因表達的時間、劑量效應以及誘導表達的可能機理，並研究axud1的過表達對細胞周期和細胞凋亡相關蛋白表達的影響； （ 3 ） axud1原核表達載體的構建及其在大腸桿菌中的表達。本實驗的主要結果和結論如下： 1利用一步法rt - pcr成功地從人類外周血淋巴細胞中克隆出axud1基因編碼區cdna ，並將其構建入真核表達載體中，編碼的ha - axud1融合蛋白帶有流感病毒凝血素ha的表位標記肽段。 -
Pcr methodology was adopted for cloning of chitinase encoding genes. based on the sequences of chitinase gene from genebank, the primers for chitinase gene amplification were designed. pcr fragment was ligated with pmd18 ~ t vector and transformed into e. coli dh5 a
盡管同源性比較低，但酶活性檢測發現該基因片段具有幾丁質酶活性，認為此pcr片段含有幾丁質酶編碼基因的全序列或部分序列，此基因片段是一個新基因或基因片段。 -
On the base of construction of pbi121vp7, we constructed the fusion gene expression vector pbi121ctbvp7 by the same sigle cloning site ( ndei and xbai ) of vector puc19ctb and pbi121vp7
設計具有ndei和saci單限制性酶切位點的vp7基因引物，通過pcr擴增出vp7基因並經測序驗證。 -
In this experiment, gene cloning, sequence analysis and construction of expression vector of goat fsh were fist studied. this work initiated the basic research of recombinant goat fsh which supplied the theoretic basis and experiment references to its quantity production and application
本研究首次進行了山羊fsh的基因克隆、序列分析及表達載體構建方面的探索，填補了國內外重組山羊fsh基礎研究的空白，為今後重組山羊fsh的大量生產應用提供了理論基礎和實驗依據。
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