gene substitution 中文意思是什麼

gene substitution 解釋
基因替代
  • gene : n. 【生物學】基因。 dominant gene顯性基因。
  • substitution : n. 1. 代，代用，代替，更替，置換。2. 【化學】取代；【數學】代換；代入；【法律】預定繼承人；【語法】詞的代用。adj. -al ,-ary adj.
  1. No trace of any newly expressed protein band was noticed in supernant as well as in the cells by sds - page, except the verification of the substitution of beta - galactosidase gene ( the lose of galactosidase protein band ), which is a selective marker of the wild - type virus. elisa test results suggested the expression of egf in cells, but not in culture supernant. the quantitative calculation suggested the expressed egf was about 6 - 7 u g ( as egf standard ) per flask ( 2 > < 106 cells ) in the cellular extract

    將重組病毒rbmbacph - egf以10moi感染bmn細胞， 72小時后收集培養細胞和上清；培養上清和經超聲波處理的細胞樣品elisa檢測發現胞內樣品中存在能與egf抗體免疫反應的產物，粗略估計表達量約6 7 g 2 10 ~ 6個細胞（相當于egf標準品） ；重組病毒rbmbacph - egf穿刺接種5齡家蠶幼蟲，每隔24h收集蠶血淋巴，經elisa檢測發現第4天表達量最高，根據egf標準曲線計算蠶血淋巴的表達量約32 g ml ； elisa定性實驗還發現正常蠶血也存在與egf抗體間交叉反應的物質。
  2. Successfully cloned and constructed infectious full - length cdna of attenuated lapinized csfv chinese - strain ( derived from spleen ) could make us get pure rna virus genome of csfv c - strain, and further study and utilize mutation, deletion, insertion and substitution of csfv gene on dna molecular level

    中國豬瘟兔化弱毒（脾淋毒）全長感染性cdna的克隆和構建，可以使我們得到純粹的csfvc -株rna病毒基因組，在dna水平上研究和利用csfv的基因突變、缺失、插入和替換。
  3. Sequence variation analysis exhibits high transition to transversion substitution ratios, and transitions and transversions are unsaturated in the sequence of cytochorme b gene. the base composition of the cytochrome b sequences was highly biased. using a structural model of the cytochrome b protein as a template, we divided cyt b sequence data for species within anserifomes into three functional domains

    3 ）序列具有較高的轉換顛換比，基因序列中的轉換與顛換都未飽和，序列中的堿基組成高度偏倚： 4 ）按照細胞色素為蛋白結構模型，細胞色素b基因全序列可分為三個功能區，堿基替換飽和狀況與不同功能區相關聯。
  4. Substitution and insertion in all strains si gene, the homogeneity of nucleotide and the deduced amino acids of s1 gene with 17 alien and domestic references strains were equally less than 80 %

    S1基因除與qx的親緣關系較近外，與其它參考株的同源率均低於80 ，可能為一株國內流行性的變異株。
  5. The results suggested that eaggec hpi - positive strains expressed the ybt system, but the presence of hpi core part does n ' t mean ybt expression. 2. research on the function of ybtp, ybtq and ybtx genes in eaggec 17 - 2 ybtp, ybtq and ybtx genes from yen wa were cloned respectively and mutated, then inserted by a selectable substitution with chloramphenicokcaf ) gene in vitro

    攜帶hpi毒力島的eaggec17毛ybtp 、 ybtq 、 ybtx基因功能研究克隆了小腸結腸炎耶爾森菌hpi毒力島一rr7和叩結構基因，在體外進行精確突變后，插入氯黴素抗性基因（ cat ）作為選擇性標記。
  6. All the subjects were genotyped by pcr - rflp ( polymerase chain reaction - restriction fragment length polymorphism ) at polymorphic sac i site inside the exon 7 of the ahsg gene. this polymorphism involves a nucleotide substitution of c to g at the middle nucleotide of the codon at amino acid position 238 resulting in the replacement of threonine ( acc ) with serine ( agc )

    所有的樣本通過聚合酶鏈式反應?限制性片段長度多態性方法（ pcr - rflp ）對ahsg基因的第7個外顯子內的sac多態性位點進行基因分型，該多態性位點為238號氨基酸密碼子中間的堿基c到g的替換，使蘇氨酸（ thr ， acc ）變為絲氨酸（ ser ， agc ） 。
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